阿托伐他汀联合吡格列酮对大鼠缺血再灌注心肌的保护作用(1)
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[摘要] 目的 探讨阿托伐他汀、吡格列酮及二者联用预处理大鼠后，对缺血再灌注(I/R)心肌的保护作用，并探讨其可能的机制。方法 选雄性健康Sprague-Dawley(SD)大鼠40只，将上述大鼠随机分为5组：假手术组(生理盐水，2mL/d，8例)、I/R组(生理盐水，2mL/d，8例)、阿托伐他汀组[阿托伐他汀，20mg/(kg·d)，8例]、吡格列酮组[吡格列酮，3mg/(kg·d)，8例]、阿托伐他汀+吡格列酮组[阿托伐他汀，20mg/(kg·d)+吡格列酮3mg/(kg·d)，8例]。灌胃1周后，第8天制作大鼠在体心肌I/R模型。缺血30min，再灌注1h后测定血清丙二醛(MDA)及超氧化物歧化酶(SOD)；Evans blue、TTC双重染色后，用图像分析软件计算梗死面积。结果 阿托伐他汀组、吡格列酮组、阿托伐他汀+吡格列酮组与I/R组比较，心肌梗死面积减少，SOD升高、MDA降低(P<0.01)。联合用药组较单独用药组心肌梗死面积减少，SOD升高，MDA降低(P<0.01)；较假手术组SOD降低，MDA升高(P<0.01)。阿托伐他汀组与吡格列酮组各指标水平接近(P>0.05)。 结论 阿托伐他汀、吡格列酮能减轻心肌缺血再灌注损伤，两药联合应用作用更加明显，其作用机制与提高血清SOD活性，降低MDA含量有关。
[关键词] 冠心病；阿托伐他汀；吡格列酮；心肌缺血再灌注；保护作用
[中图分类号] R965 [文献标识码] A [文章编号] 1673-9701(2011)36-04-02
Protective Effect of Atorvastatin and Pioglitazone on Ischemia and Reperfusion Myocardium in Rats
ZU Yugang CHEN Huanzhen
Department of Cardiology，the First Hospital of Shanxi Medical University， Taiyuan 030001，China
[Abstract] Objective To investigate atorvastatin，pioglitazone and two drugs in combination on ischemia-reperfusion myocardial protective effects and the possible mechanism. Methods All 40 healthy SD rats were randomly divided into 5 groups：sham operation group(n=8，0.9%sodium chloride injection liquid，2mL/d)，I/R group(n=8，0.9% sodium chloride injection liquid，2mL/d)，atorvastatin group[(n=8，atorvastatin，20mg/kg·d)]，pioglitazone group[n=8，pioglitazone 3mg/(kg·d)]，atorvastatin + pioglitazone group[n=8，atorvastat in，20mg/(kg·d)+pioglitazone 3mg/(kg·d)].After one week gavage make the rat model of myocardial ischemia-reperfusion in eight days，ischemia 30minute，one hour after reperfusion serum malondialdehyde(MDA) and superoxide dismutase(SOD).Evans blue，TTC double staining，using image analysis software to calculate infarct size. Results The atorvastatin group，pioglitazone group，atorvastatin + pioglitazone group and I/R group，infarct size reduction，SOD increased，MDA decreased(P<0.01).Atorvastatin + pioglitazone group than in the atorvastatin group，pioglitazone group myocardial infarct size reduction，SOD increased ......
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