中介素1-53对急性肺损伤大鼠SP-A及IL-18的影响(1)
 |
| 第1页 |
参见附件(2855KB，3页)。
[摘要] 目的 探讨脂多糖致急性肺损伤大鼠肺泡灌洗液(BALF)中表面蛋A(pulmonary surfactant associated protein A，SP-A)浓度及肺组织单核巨噬细胞中白介素18(interleukin 18，IL-18)的表达水平及中介素1-53(intermedin1-53，IMD1-53)的影响。 方法 将36只雄性Vistar大鼠随机分为正常组(A组)、急性肺损伤组(B组)、中介素1-53干预组(C组)，于2 h，4 h分别测定血气分析，后处死大鼠，测定肺组织湿/干比(W/D)、ELISA法检测BALF中SP-A的浓度、免疫组化法检测肺单核巨噬细胞内IL-18表达及各时间点肺组织病理变化。 结果 与A组比较，B组动脉血PO2和BALF中SP-A浓度降低显著(P < 0.05)，湿干比及单核巨噬细胞中IL-18表达增加(P < 0.05)，肺组织充血水肿明显。与C组相比，上述改变得以逆转，肺损伤程度明显减轻(P < 0.05)。 结论 中介素1-53可通过抑制SP-A的减少和IL-18的生成在急性肺损伤早期起到一定的保护作用。
[关键词] 急性肺损伤；中介素1-53；SP-A；IL-18
[中图分类号] R563 [文献标识码] A [文章编号] 1673-9701(2012)03-0001-03
Effects of intermedin 1-53 on SP-A and IL-18 in LPS-induced acute lung injury septic rats
LIU Ling1 LI Jianqiang2 SHI Jing1 ZHAO Hui2
1.Shanxi Medical University, Taiyuan 030001, China; 2.Department of Respiration, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
[Abstract] Objective To explore the influence of intermedin1-53 on the leve of SP-A in BALF and IL-18 expressions of alveolar macrophages in lipopolysaccharide of LPS-induced acute lung injury (ALI) in rats. Methods All of 36 male Wistar rats were randomly divided into 3 groups: normal control (A), LPS-treated group (B), intermediary 1-53 intervention group (C). Blood samp leswere taken for blood gas analysis at 2, 4 h.The animals were killed after 4 h, the ratio of wet to dry lung weight was measured. The concentration of SP-A in BALF with ELISA method. The exression of IL-18 was examined by immunohistochemical method and the changes of lung tissue were pathol ogically observed. Results Compared with group A, PO2 and SP-A had a decreased significantly in 2 and 4 hours in group B (P < 0.05). The ratio of wet to dry lung weight and the expressi of IL-18 alveolar macrophages in lipopolysaccharide had a increased significantly in group B (P < 0.05), The degree of lung injury was hardly. These changes were inverted in group C, and the degree of lung injury was markedly at tenuated (P < 0 ......
您现在查看是摘要介绍页，详见PDF附件(2855KB，3页)。