TGF-β1、CTGF在胆管缺血性损伤修复过程中的表达及意义(1)
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[摘要] 目的 探讨转化生长因子β1(TGF-β1)及结缔组织生长因子(CTGF)在胆管缺血性损伤修复过程中的表达及意义。 方法 建立大鼠胆管缺血损伤修复模型，随机分为对照组和实验组，并分别于术后1、3、5和8周后取材，运用免疫组化技术检测胆管组织中TGF-β1和CTGF的阳性表达部位，并采用病理图像分析系统计算胆管缺血性损伤修复过程中各组阳性细胞数。 结果 对照组表现为胶原纤维的平行密集排列，未见明显的无炎性细胞浸润；实验组胶原纤维增生明显、层次紊乱，疏松结缔组织中可见大量毛细血管及炎性细胞浸润；TGF-β1及CTGF在术后各期均有显著表达，但术后各期表达对比差异无统计学意义(P > 0.05)；TGF-β1及CTGF在正常对照组无表达或者仅轻微表达，与术后各期TGF-β1及CTGF表达比较差异有统计学意义(P < 0.01)；Spearman相关分析：实验组术后各期TGF-β1和CTGF的表达呈正相关性。 结论 TGF-β1和CTGF在胆管缺血损伤修复过程中相伴表达且与良性胆管狭窄的形成密切相关，为防治瘢痕增生和良性胆管狭窄提供了一条新的思路和途径。
[关键词] 损伤修复；胆管狭窄；转化生长因子β1；结缔组织生长因子
[中图分类号] R657.4+6 [文献标识码] A [文章编号] 1673-9701(2012)04-0006-03
Expression and significance of TGF-β1 and CTGF in the repairing process of bile duct ischemic injury
WANG Yong1 WANG Xiangyu2 LI Lijun2 HU Chunlei2 GAO Feng1
1.Department of General Surgery, the Third People’s Hospital of Ouhai District of Wenzhou, Wenzhou 325014, China; 2.Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China
[Abstract] Objective To explore the expression and significance of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF) in the repairing process of biliary ischemic injury. Methods Rat bile duct ischemia injury and repair modes were established and randomly divided into control group and experimental group, and after operation respectively 1, 3, 5 and 8 weeks after sampling, using immunohistochemical techniques for the detection of bile duct tissue TGF-β1 and the positive expression of CTGF site, and the use of image analysis system to calculate the number of positive cells of the bile duct ischemia injury repair process in each group. Results The control group showed collagen fibers parallel arranged densely, no obvious without inflammatory cell infiltration; experimental groups of collagen fibers proliferated markedly, levels of disturbance, loose connective tissue revealed numerous capillaries and infiltration of inflammatory cells; TGF β1 and CTGF in postoperative periods were significantly expressed, but after the voicing contrast there was no significant difference (P > 0 ......
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