牛磺酸对严重腹腔感染大鼠肠黏膜屏障功能的影响(1)
[摘要] 目的 通过建立大鼠严重腹腔感染模型，检测血浆二胺氧化酶(diamine oxidase，DAO)的活性及D-乳酸含量，探讨牛磺酸对严重腹腔感染大鼠肠黏膜屏障的保护作用。 方法 实验于2015年5～7月将54只雄性SD大鼠随机分为三组：对照组(C组)、腹腔感染组(I组)和治疗组(T组)，每组18只。C组仅轻微翻动盲肠后关腹，I、T两组采用盲肠结扎穿孔法(cecal ligation plus puncture，CLP)。T组于术后即刻腹腔注射牛磺酸溶液(200 mg/kg)，而C组、I组术后即刻腹腔注射等体积生理盐水。各组分别于造模后6、12、24 h随机取6只大鼠，检测血浆DAO活性和D-乳酸含量。 结果 C组各检测指标在各时间点无明显差异，差异无统计学意义(P>0.05)，I组、T组各检测指标均随时间延长呈增高趋势。I组、T组各检测指标在各时间点均高于C组，差异均有统计学意义(P<0.05)，T组各检测指标在各时间点均低于I组，差异均有统计学意义(P<0.05)。 结论 牛磺酸对大鼠严重腹腔感染时肠黏膜屏障功能有一定的保护作用。
[关键词] 牛磺酸；腹腔感染；肠黏膜屏障；二胺氧化酶；D-乳酸
[中图分类号] R605 [文献标识码] A [文章编号] 1673-9701(2016)07-0022-03
[Abstract] Objective To explore the possible protective effect of taurine on intestinal mucosal barrier function in rats with severe abdominal infection through established the model of rats with severe abdominal infection， measuring the activity of diamine oxidase(DAO) and the concentration of D-lactic acid in plasma. Methods From May to July 2015 fifty-four SD males rats were randomly divided into three groups， 18 rats in each group)： control group (group C)， abdominal infection group(group I) and treatment group(group T). The rats of group C were only slightly changed the cecal several times before definitive abdominal closure. The rats in group I and group T were established by cecal ligation and puncture (CLP). The rats in group T received intraperitoneal injection of taurine(200 mg/kg) immediately after the operation， however， the rats in group C and group I received intraperitoneal injection of equivoluminal normal saline. Six rats in each group were randomly picked up at 6， 12， 24 h after operation to have the activity of diamine oxidase (DAO) and the concentration of D-lactic acid in plasma measured. Results There was no significant difference at each time point of each detection index in group C(P>0.05). In group I and group T， each detection index was rising with increased postoperative time， and they were higher than those of group C at each time point， there was significant difference(P<0.05). Compared with group I， each detection index of group T was lower at each time point， there was significant difference(P<0.05). Conclusion Taurine has a certain protective effect on intestinal mucosal barrier function in rats with severe abdominal infection.
[Key words] Taurine； Abdominal infection； Intestinal mucosal barrier； Diamine oxidase； D-lactic acid
腹腔感染在普通外科较为常见，多由手术、创伤、消化道穿孔、急性胰腺炎、肠外瘘等原因导致。严重腹腔感染时，肠黏膜在各种致病因素作用下出现不同程度的损伤，通透性有所增加，肠道内的细菌(多为革兰阴性杆菌)和内毒素从肠腔经门静脉、淋巴循环移位可进一步引发全身炎症反应综合征(systemic inflammatory response syndrome，SIRS)、脓毒症、甚至多器官功能障碍综合征(multiple organ dysfunction syndrome，MODS)[1]，病情严重，治疗费用昂贵。牛磺酸是一种由含硫氨基酸转化而来的β-氨基酸，由于其分子结构中的羧酸基被磺酸基取代，所以牛磺酸并不参与蛋白质的合成。急性炎症反应时，由于中性粒细胞嗜天青颗粒内髓过氧化物酶(myeloperoxidase，MPO)的催化作用，细胞内氧化应激产生的H2O2与Cl-、Br-反应生成次氯酸、次溴酸，而牛磺酸与两者相互作用，减轻了次氯酸、次溴酸对正常组织的氧化损伤，并生成具有杀菌、抗炎、抑制氧化应激的作用的牛磺酸氯胺、牛磺酸溴胺[2]。本实验通过多时间点比较各组大鼠血浆DAO活性、D-乳酸含量变化，探讨牛磺酸对大鼠腹腔感染早期肠黏膜屏障功能的保护作用，为将来临床应用提供一定依据。, http://www.100md.com(蔡景融 武华 程巩)
[关键词] 牛磺酸；腹腔感染；肠黏膜屏障；二胺氧化酶；D-乳酸
[中图分类号] R605 [文献标识码] A [文章编号] 1673-9701(2016)07-0022-03
[Abstract] Objective To explore the possible protective effect of taurine on intestinal mucosal barrier function in rats with severe abdominal infection through established the model of rats with severe abdominal infection， measuring the activity of diamine oxidase(DAO) and the concentration of D-lactic acid in plasma. Methods From May to July 2015 fifty-four SD males rats were randomly divided into three groups， 18 rats in each group)： control group (group C)， abdominal infection group(group I) and treatment group(group T). The rats of group C were only slightly changed the cecal several times before definitive abdominal closure. The rats in group I and group T were established by cecal ligation and puncture (CLP). The rats in group T received intraperitoneal injection of taurine(200 mg/kg) immediately after the operation， however， the rats in group C and group I received intraperitoneal injection of equivoluminal normal saline. Six rats in each group were randomly picked up at 6， 12， 24 h after operation to have the activity of diamine oxidase (DAO) and the concentration of D-lactic acid in plasma measured. Results There was no significant difference at each time point of each detection index in group C(P>0.05). In group I and group T， each detection index was rising with increased postoperative time， and they were higher than those of group C at each time point， there was significant difference(P<0.05). Compared with group I， each detection index of group T was lower at each time point， there was significant difference(P<0.05). Conclusion Taurine has a certain protective effect on intestinal mucosal barrier function in rats with severe abdominal infection.
[Key words] Taurine； Abdominal infection； Intestinal mucosal barrier； Diamine oxidase； D-lactic acid
腹腔感染在普通外科较为常见，多由手术、创伤、消化道穿孔、急性胰腺炎、肠外瘘等原因导致。严重腹腔感染时，肠黏膜在各种致病因素作用下出现不同程度的损伤，通透性有所增加，肠道内的细菌(多为革兰阴性杆菌)和内毒素从肠腔经门静脉、淋巴循环移位可进一步引发全身炎症反应综合征(systemic inflammatory response syndrome，SIRS)、脓毒症、甚至多器官功能障碍综合征(multiple organ dysfunction syndrome，MODS)[1]，病情严重，治疗费用昂贵。牛磺酸是一种由含硫氨基酸转化而来的β-氨基酸，由于其分子结构中的羧酸基被磺酸基取代，所以牛磺酸并不参与蛋白质的合成。急性炎症反应时，由于中性粒细胞嗜天青颗粒内髓过氧化物酶(myeloperoxidase，MPO)的催化作用，细胞内氧化应激产生的H2O2与Cl-、Br-反应生成次氯酸、次溴酸，而牛磺酸与两者相互作用，减轻了次氯酸、次溴酸对正常组织的氧化损伤，并生成具有杀菌、抗炎、抑制氧化应激的作用的牛磺酸氯胺、牛磺酸溴胺[2]。本实验通过多时间点比较各组大鼠血浆DAO活性、D-乳酸含量变化，探讨牛磺酸对大鼠腹腔感染早期肠黏膜屏障功能的保护作用，为将来临床应用提供一定依据。, http://www.100md.com(蔡景融 武华 程巩)