魏 丽 刘萌萌 屈彩霞

    郑州市第三人民院病理科，河南郑州450000

    TGF-β1与α-SMA在小鼠肺纤维化模型中的表达及其相互关系

    魏丽刘萌萌屈彩霞

    郑州市第三人民院病理科，河南郑州450000

    目的探讨转化生长因子β1(TGF-β1)与α-平滑肌肌动蛋白(α-SMA)在小鼠肺纤维化模型中的表达，及其之间的相互关系。方法气管内灌注博莱霉素(7 mg/kg)制备肺纤维化模型，进行HE、Masson染色并采用免疫组织化学检测小鼠肺纤维化组织中TGF-β1和α-SMA的表达。结果对照组中α-SMA表达于支气管和大血管周围的平滑肌细胞，模型组中α-SMA表达于支气管、大血管周围的平滑肌细胞及成纤维细胞灶，TGF-β1表达于支气管、大血管周围的成纤维细胞、炎细胞以及成纤维细胞灶，与α-SMA表达相似。TGF-β1和α-SMA均与小鼠肺纤维化程度均呈正相关(r=0.628、0.766，P<0.01)。TGF-β1与α-SMA呈正相关(rs=0.728，P<0.01)。结论TGF-β1和α-SMA在肺间质纤维形成过程起到重要作用，并可能协同参与。

    博莱霉素；肺纤维化；TGF-β1；α-SMA；小鼠

    [Abstract]Objective To discuss the expression of and relation between TGF-β1 and α-SMA in mice pulmonary fibrosis model.Methods The pulmonary fibrosis model was prepared by intratracheal instillation of bleomycin(7 mg/kg). The expression of TGF-β1and α-SMA in mice pulmonary fibrosis tissues was detected by HE and Masson staining and immunohistochemistry.Results In the control group，α-SMA was expressed in smooth muscle cells of bronchi and around great vessels，while in the model group，α-SMA was expressed in smooth muscle cells，fibroblasts，and fibroblast foci of bronchi and around great vessels ......