11周龄硝酸甘油大鼠模型表面效度研究(1)
[摘要] 目的 本研究旨在11周龄硝酸甘油偏头痛模型大鼠头痛行为学的基础上结合临床偏头痛的头痛症状学对偏头痛大鼠模型的表面效度进行研究。 方法 根据体重及基础痛阈将36只大鼠随机分成四组，即正常组、模型组、西药组(苯甲酸利扎曲普坦)、中药组(川芎-天麻)，每组9只。造模后，用高清摄像头记录大鼠行为学2 h。通过空白对照和药物反证治疗进行系统评价。 结果 造模后2 h内，模型组爬笼及理毛次数少于正常组，挠头次数多于正常组。西药组及中药组挠头次数少于模型组。 结论 挠头次数可以作为评价偏头痛大鼠模型表面效度的核心内容及判断药物疗效的指标。同时发现，11周龄偏头痛大鼠行为可能与人类偏头痛症状更相近。
[关键词] 偏头痛；硝酸甘油；表面效度；周龄
[中图分类号] R747.2 [文献标识码] A [文章编号] 1673-9701(2017)25-0033-04
Study on the surface validity of 11-week-old nitroglycerin rat model
ZHOU Huixia1，2 ZHANG Zhengxiang3 CHEN Qiang3 KONG Minlu4 DENG Yu1 CHENG Jing1 HOU Qun3 SHAO Minfeng1
1.No.1 School of Clinical Medicine， Zhejiang Chinese Medical University， Hangzhou 310053， China； 2.Department of Neurology， the First Affiliated Hospital of Zhejiang Chinese Medical University， No.1 School of Clinical Medicine， Zhejiang Chinese Medical University， Zhejiang Provincial Hospital of TCM， Hangzhou 310006， China； 3.Zhejiang Xiaoshan Hospital， Hangzhou 311202， China； 4.Hangzhou Geriatric Disease Hospital， Hangzhou 310022， China
[Abstract] Objective To study the surface validity of migraine rats on the basis of the headache behavioral science of 11-week-old nitroglycerin migraine rate model combined with the symptomatology of clinical migraine. Methods 36 rats were randomly divided into 4 groups according to body weight and basic pain threshold， namely normal group， model group， Western medicine group(Rizatriptan)， and traditional Chinese medicine group(ligusticum wallichii-gastrodia elata)， with 9 rats in each group. After modeling， the behavior of rats was recorded by high-definition camera for 2 hours. A systematic review was conducted by blank control and drug counterevidence therapy. Results Within 2 hours after modeling， the frequency of climbing cage and grooming in the model group was less than that in the normal group， and the frequency of scratching head was more than that in the normal group. The frequency of scratching head in the Western medicine group and Chinese medicine group was less than that in the model group. Conclusion Scratching head can be used as the core content of evaluating surface validity of migraine rats and the index of judging drug efficacy. At the same time， the behavior of 11-week-old migraine rats are found to be more likely to be associated with human migraine symptoms.
[Key words] Migraine； Nitroglycerin； Surface validity； Week age
偏頭痛是一种常见的慢性神经血管性疾病，我国患病率为9.3%[1]，好发于中青年，40岁[2]达最高峰，40岁后发病率开始下降。2013年全球疾病负担调查的研究结果表明偏头痛为人类第三位常见疾病，按失能所致生命年损失(years of life lost to disability，YLDs)计算，偏头痛为第六位致残性疾病[3]。硝酸甘油模型具有操作简单、机制单一、短时间内可大量复制等优点，且其行为学表现及病理生理变化与人类发病时的表现及变化相似，因此被广泛应用于偏头痛相关课题的研究。本研究选用11周龄[4，5]大鼠通过视频录像方法对硝酸甘油大鼠模型行为学进行系统评价。, 百拇医药(周会霞 章正祥 陈强 孔敏露 邓玉 程静 侯群 邵敏峰)
[关键词] 偏头痛；硝酸甘油；表面效度；周龄
[中图分类号] R747.2 [文献标识码] A [文章编号] 1673-9701(2017)25-0033-04
Study on the surface validity of 11-week-old nitroglycerin rat model
ZHOU Huixia1，2 ZHANG Zhengxiang3 CHEN Qiang3 KONG Minlu4 DENG Yu1 CHENG Jing1 HOU Qun3 SHAO Minfeng1
1.No.1 School of Clinical Medicine， Zhejiang Chinese Medical University， Hangzhou 310053， China； 2.Department of Neurology， the First Affiliated Hospital of Zhejiang Chinese Medical University， No.1 School of Clinical Medicine， Zhejiang Chinese Medical University， Zhejiang Provincial Hospital of TCM， Hangzhou 310006， China； 3.Zhejiang Xiaoshan Hospital， Hangzhou 311202， China； 4.Hangzhou Geriatric Disease Hospital， Hangzhou 310022， China
[Abstract] Objective To study the surface validity of migraine rats on the basis of the headache behavioral science of 11-week-old nitroglycerin migraine rate model combined with the symptomatology of clinical migraine. Methods 36 rats were randomly divided into 4 groups according to body weight and basic pain threshold， namely normal group， model group， Western medicine group(Rizatriptan)， and traditional Chinese medicine group(ligusticum wallichii-gastrodia elata)， with 9 rats in each group. After modeling， the behavior of rats was recorded by high-definition camera for 2 hours. A systematic review was conducted by blank control and drug counterevidence therapy. Results Within 2 hours after modeling， the frequency of climbing cage and grooming in the model group was less than that in the normal group， and the frequency of scratching head was more than that in the normal group. The frequency of scratching head in the Western medicine group and Chinese medicine group was less than that in the model group. Conclusion Scratching head can be used as the core content of evaluating surface validity of migraine rats and the index of judging drug efficacy. At the same time， the behavior of 11-week-old migraine rats are found to be more likely to be associated with human migraine symptoms.
[Key words] Migraine； Nitroglycerin； Surface validity； Week age
偏頭痛是一种常见的慢性神经血管性疾病，我国患病率为9.3%[1]，好发于中青年，40岁[2]达最高峰，40岁后发病率开始下降。2013年全球疾病负担调查的研究结果表明偏头痛为人类第三位常见疾病，按失能所致生命年损失(years of life lost to disability，YLDs)计算，偏头痛为第六位致残性疾病[3]。硝酸甘油模型具有操作简单、机制单一、短时间内可大量复制等优点，且其行为学表现及病理生理变化与人类发病时的表现及变化相似，因此被广泛应用于偏头痛相关课题的研究。本研究选用11周龄[4，5]大鼠通过视频录像方法对硝酸甘油大鼠模型行为学进行系统评价。, 百拇医药(周会霞 章正祥 陈强 孔敏露 邓玉 程静 侯群 邵敏峰)