炎症因子在新生儿缺氧缺血性脑病大鼠脑组织中的表达及意义(1)
[摘要] 目的 探讨炎症因子在新生儿缺氧缺血性脑病(hypoxic-ischemic encephalopathy，HIE)大鼠脑组织中的表达及意义。 方法 36只新生SD大鼠，随机分为对照组、缺血缺氧0 h、6 h、1 d、3 d、7 d组，每组6只。观察各组大鼠脑组织中 TNF-α、CXCL10、CXCL12、 IL-1α、IL-1β、IL-1R mRNA表达差异。 结果 TNF-α在HIE发生后开始增高，6～24 h达到高峰后逐渐下降，72 h仍高于正常，而后下降，但仍高于对照组，至7 d后呈现低表达。CXCL10/12在0 h时表达与对照组对比未见差异，在6 h及12 h达到高峰，至72 h逐渐下降但仍高于对照组，至7 d时基本降至正常。相对对照组，IL-1R 在整个HIE过程中未见明显高表达，而在疾病的初期及恢复期其发病早期却出乎意料的呈现低表达的特点(0 h、24 h、72 h)，至7 d后恢复正常。IL-1α、IL-1β在0 h时期呈现低表达，6 h呈现高表达，而后在24 h后恢复正常，至7 d后IL-1α复又呈现低表达趋势。 结论 炎症因子与HIE发病进展密切相关，并且在疾病的不同进程中各种炎症因子起着不同的作用，可以作为判断疾病进展的客观指标。
[关键词] 新生儿缺血缺氧性脑病；TNF-α；CXCL；白细胞介素
[中图分类号] R722.1 [文献标识码] A [文章编号] 1673-9701(2018)28-0038-04
[Abstract] Objective To investigate the expression and significance of inflammatory factors in the brain tissues of neonatal rats with hypoxic-ischemic encephalopathy(HIE). Methods 36 newborn SD rats were randomly divided into control group， and hypoxic-ischemic 0 h， 6 h， 1 d， 3 d， 7 d groups， with 6 rats in each group. The differences in the expressions of TNF-α， CXCL10， CXCL12， IL-1α， IL-1β and IL-1R mRNA were observed in the brain tissues of rats in each group. Results TNF-α was increased after HIE， peaked at 6-24 h and was then decreased， and was still higher than normal at 72 h， and was then decreased. However， it was still higher than the control group and showed low expression after 7 d. There was no difference in CXCL10/12 expression at 0 h compared with the control group， peaked at 6 h and 12 h， and was gradually decreased at 72 h but still higher than the control group. It was basically decreased to normal at 7 d. Compared with the control group， IL-1R did not show significant high expression during the whole HIE process. In the early stage of the disease and in the stage of recovery， it unexpectedly showed low expression (0 h， 24 h， 72 h) at the early stage of onset， and returned to normal after 7 d. IL-1α and IL-1β showed low expression at 0 h but turned to high expression at 6 h， and then returned to normal after 24 h， but IL-1α showed a low expression trend after 7 d. Conclusion Inflammatory factors are closely related to the progression of HIE， and various inflammatory factors play different roles in different processes of the disease， which can be used as an objective indicator to judge disease progression.
[Key words] Neonatal hypoxic-ischemic encephalopathy (HIE)； TNF-α； CXCL； Interleukin
新生兒缺氧缺血性脑病(hypoxic-ischemic encephalopathy，HIE)指围产期新生儿因缺氧引起的脑部病变，主要由宫内窘迫、新生儿窒息缺氧引起，是造成新生儿死亡和不同程度的智力低下、脑瘫等神经系统发育障碍的主要原因[1，2]。HIE发病机制及脑损害后神经重塑机制一直未明[3，4]。有研究证明，炎症因子参与HIE的免疫损伤及神经重塑，炎症因子表达与疾病发展程度相关亦与神经系统损伤程度密切相关[5，6]。本研究采取更为准确的实验方法检测炎症因子的表达及动态观察不同时间段的表达，为以后的临床检测、治疗及下一步在分子学及细胞学研究提供研究基础及可行性。, 百拇医药(曾志涌 卢顺玉 黎云鹏)
[关键词] 新生儿缺血缺氧性脑病；TNF-α；CXCL；白细胞介素
[中图分类号] R722.1 [文献标识码] A [文章编号] 1673-9701(2018)28-0038-04
[Abstract] Objective To investigate the expression and significance of inflammatory factors in the brain tissues of neonatal rats with hypoxic-ischemic encephalopathy(HIE). Methods 36 newborn SD rats were randomly divided into control group， and hypoxic-ischemic 0 h， 6 h， 1 d， 3 d， 7 d groups， with 6 rats in each group. The differences in the expressions of TNF-α， CXCL10， CXCL12， IL-1α， IL-1β and IL-1R mRNA were observed in the brain tissues of rats in each group. Results TNF-α was increased after HIE， peaked at 6-24 h and was then decreased， and was still higher than normal at 72 h， and was then decreased. However， it was still higher than the control group and showed low expression after 7 d. There was no difference in CXCL10/12 expression at 0 h compared with the control group， peaked at 6 h and 12 h， and was gradually decreased at 72 h but still higher than the control group. It was basically decreased to normal at 7 d. Compared with the control group， IL-1R did not show significant high expression during the whole HIE process. In the early stage of the disease and in the stage of recovery， it unexpectedly showed low expression (0 h， 24 h， 72 h) at the early stage of onset， and returned to normal after 7 d. IL-1α and IL-1β showed low expression at 0 h but turned to high expression at 6 h， and then returned to normal after 24 h， but IL-1α showed a low expression trend after 7 d. Conclusion Inflammatory factors are closely related to the progression of HIE， and various inflammatory factors play different roles in different processes of the disease， which can be used as an objective indicator to judge disease progression.
[Key words] Neonatal hypoxic-ischemic encephalopathy (HIE)； TNF-α； CXCL； Interleukin
新生兒缺氧缺血性脑病(hypoxic-ischemic encephalopathy，HIE)指围产期新生儿因缺氧引起的脑部病变，主要由宫内窘迫、新生儿窒息缺氧引起，是造成新生儿死亡和不同程度的智力低下、脑瘫等神经系统发育障碍的主要原因[1，2]。HIE发病机制及脑损害后神经重塑机制一直未明[3，4]。有研究证明，炎症因子参与HIE的免疫损伤及神经重塑，炎症因子表达与疾病发展程度相关亦与神经系统损伤程度密切相关[5，6]。本研究采取更为准确的实验方法检测炎症因子的表达及动态观察不同时间段的表达，为以后的临床检测、治疗及下一步在分子学及细胞学研究提供研究基础及可行性。, 百拇医药(曾志涌 卢顺玉 黎云鹏)