复方芪苓配方颗粒对大鼠高尿酸血症的调控作用(1)
[摘要] 目的 探讨复方芪苓配方颗粒治疗高尿酸血症大鼠的作用机制。 方法 通过腺嘌呤灌胃及氧嗪酸钾皮下注射建立高尿酸血症模型，连续给药14 d。分别测定血清尿酸(UA)、肌酐(Scr)、尿素(Urea)及胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-c)和低密度脂蛋白(LDL-c)水平;采用RT-PCR方法分别测定大鼠肾脏中相关转运体的表达水平。 结果 与模型对照组比较，复方芪苓配方颗粒能显著降低高尿酸血症大鼠血清UA、Scr、Urea、TC、LDL-c水平，同时可以显著下调肾脏尿酸盐转运体(URAT1)mRNA的表达，上调有机阴离子转运体1(OAT1)、有机阳离子转运体2(OCT2)mRNA的表达。 结论 复方芪苓配方颗粒能改善高尿酸血症大鼠的肾功能、血脂代谢，其作用机制可能是下调大鼠肾脏中URAT1 mRNA的水平，上调OAT1和OCT2 mRNA的水平，从而降低肾脏尿酸盐重吸收能力及增加尿酸盐的分泌。
[关键词] 复方芪苓配方颗粒;高尿酸血症;肾脏转运体;大鼠
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701(2019)33-0008-04
[Abstract] Objective To explore the mechanism of action of compound Qiling formula granules in treating rats with hyperuricemia. Methods The hyperuricemia model was established in rats by adenine gavage and hypodermic injection of oxonic acid potassium salt. The drug was administered continuously for 14 days. The levels of serum uric acid(UA)， creatinine(Scr)， Urea， total cholesterol(TC)， triglyceride(TG)， high-density lipoprotein-cholesterol(HDL-c)， and low-density lipoprotein-cholesterol(LDL-c) were tested respectively. RT-PCR was used to measure the expression levels of related renal transporters of the subjects. Results Compared with the model control group， the levels of serum UA， Scr， Urea， TC， LDL-c of rats with hyperuricemia were significantly lowered by applying compound Qiling formula granules. The expression level of the mRNA of the urate reabsorption transporter-1(URAT1) was down-regulated significantly， and the expression levels of the mRNA of organic anion transporter-1(OAT1) and organic cation transporter-2(OCT2) were up-regulated significantly. Conclusion Compound Qiling formula granule can improve the renal function and lipid metabolism of rats with hyperuricemia. Its mechanism may be related to the down-regulation of the level of URAT1 mRNA， and the up-regulation of the levels of OAT1 and OCT2 mRNA in the kidney of the rats， both of which can reduce the reabsorption capacity of urate in the kidney and increase the secretion of urate.
[Key words] Compound Qiling formula granules; Hyperuricemia; Renal transporter; Rats
隨着社会经济发展及人们饮食结构的改变，高尿酸血症的发病率呈明显上升趋势，且发病年龄逐渐年轻化[1-3]。高尿酸血症可诱导痛风、肾结石及加速肾脏和心血管疾病的进程[4-5]，因此对于高尿酸血症早发现、早治疗就尤为重要。西药在治疗高尿酸血症上虽有确切的降尿酸作用，但停药后复发率较高，且不良反应较多，部分药物在一定程度上有加重肾损害的风险[6]。随着中医药对高尿酸血症研究的不断深入，多个研究证明许多中药具有降低血尿酸的功效[7-8]，且中药复方制剂具有增效减毒的优势，因此，开发研究降低尿酸的中药复方制剂具有重大意义和广阔前景。
复方芪苓配方颗粒(原名：尿酸利仙配方颗粒)为徐红教授临床验方，已有多年临床应用经验，由山慈菇、土茯苓、桑寄生、丹参、黄芪、秦艽、薏苡仁等组方而成，具有健脾益气、化瘀泄浊、清热利湿的功能。本课题组前期临床研究证实本方能有效降低患者血尿酸水平，且无明显不良反应[9]。本研究拟通过复方芪苓配方颗粒对高尿酸血症大鼠的实验研究，观察其对调节肾功能、血脂代谢、肾脏转运体方面的影响，进而为临床治疗提供实验依据。, http://www.100md.com(张媛 徐红)
[关键词] 复方芪苓配方颗粒;高尿酸血症;肾脏转运体;大鼠
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701(2019)33-0008-04
[Abstract] Objective To explore the mechanism of action of compound Qiling formula granules in treating rats with hyperuricemia. Methods The hyperuricemia model was established in rats by adenine gavage and hypodermic injection of oxonic acid potassium salt. The drug was administered continuously for 14 days. The levels of serum uric acid(UA)， creatinine(Scr)， Urea， total cholesterol(TC)， triglyceride(TG)， high-density lipoprotein-cholesterol(HDL-c)， and low-density lipoprotein-cholesterol(LDL-c) were tested respectively. RT-PCR was used to measure the expression levels of related renal transporters of the subjects. Results Compared with the model control group， the levels of serum UA， Scr， Urea， TC， LDL-c of rats with hyperuricemia were significantly lowered by applying compound Qiling formula granules. The expression level of the mRNA of the urate reabsorption transporter-1(URAT1) was down-regulated significantly， and the expression levels of the mRNA of organic anion transporter-1(OAT1) and organic cation transporter-2(OCT2) were up-regulated significantly. Conclusion Compound Qiling formula granule can improve the renal function and lipid metabolism of rats with hyperuricemia. Its mechanism may be related to the down-regulation of the level of URAT1 mRNA， and the up-regulation of the levels of OAT1 and OCT2 mRNA in the kidney of the rats， both of which can reduce the reabsorption capacity of urate in the kidney and increase the secretion of urate.
[Key words] Compound Qiling formula granules; Hyperuricemia; Renal transporter; Rats
隨着社会经济发展及人们饮食结构的改变，高尿酸血症的发病率呈明显上升趋势，且发病年龄逐渐年轻化[1-3]。高尿酸血症可诱导痛风、肾结石及加速肾脏和心血管疾病的进程[4-5]，因此对于高尿酸血症早发现、早治疗就尤为重要。西药在治疗高尿酸血症上虽有确切的降尿酸作用，但停药后复发率较高，且不良反应较多，部分药物在一定程度上有加重肾损害的风险[6]。随着中医药对高尿酸血症研究的不断深入，多个研究证明许多中药具有降低血尿酸的功效[7-8]，且中药复方制剂具有增效减毒的优势，因此，开发研究降低尿酸的中药复方制剂具有重大意义和广阔前景。
复方芪苓配方颗粒(原名：尿酸利仙配方颗粒)为徐红教授临床验方，已有多年临床应用经验，由山慈菇、土茯苓、桑寄生、丹参、黄芪、秦艽、薏苡仁等组方而成，具有健脾益气、化瘀泄浊、清热利湿的功能。本课题组前期临床研究证实本方能有效降低患者血尿酸水平，且无明显不良反应[9]。本研究拟通过复方芪苓配方颗粒对高尿酸血症大鼠的实验研究，观察其对调节肾功能、血脂代谢、肾脏转运体方面的影响，进而为临床治疗提供实验依据。, http://www.100md.com(张媛 徐红)