赵学正 高伟 唐保永 郑思化 李利军

    [關键词] 5-羟基-4'-硝基-7-丙酰氧基金雀异黄素;增殖;迁移;侵袭;凋亡

    [中图分类号] R738.1? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-9701(2021)16-0037-06

    Effects and mechanisms of novel genistein derivatives in inhibiting the migration and invasion of osteosarcoma MG-63 cells

    ZHAO Xuezheng? ?GAO Wei? ?TANG Baoyong? ?ZHENG Sihua? ?LI Lijun

    Department of Orthopedics， Hangzhou Xixi Hospital， Hangzhou? ?310023， China

    [Abstract] Objective To detect the inhibitory effect of novel genistein derivatives HNPG on the migration and invasion of osteosarcoma MG-63 cells and its possible molecular biological mechanism. Methods Osteosarcoma MG-63 cells were cultured in vitro，and MTT was used to detect the proliferation inhibitory effect of HNPG on MG-63 cells. The scratch test was used to detect the migration inhibitory effect of HNPG on MG-63 cells. The Transwell was used to detect the inhibitory effect of the invasion of HNPG on MG-63 cells. PI staining FCM was used to detect the blocking effect of HNPG on the G1 phase of MG-63 cells. AV-PI staining FCM was used to detect the apoptosis induction effect of HNPG on MG-63 cells. Western blotting was used to detect the regulatory effect of HNPG on the expression of p53 ......