申永超 林佳 冯琪 朱大荣

    [摘要] 目的 探讨miR-92b与信号通路PI3K/Akt在糖尿病肾病中的相关性。 方法 选取8周龄的C57BL/6 雄性小鼠12只，随机分为正常对照组和糖尿病肾病组，利用枸橼酸钠建立糖尿病肾病小鼠模型;选取人肾小管上皮HK-2细胞12瓶，随机分为高糖组、低糖组和左旋葡萄糖组，用高糖刺激建立糖尿病肾病细胞模型。利用实时荧光定量PCR检测小鼠肾脏组织和细胞中miR-92b表达水平，Western blot检测小鼠肾脏组织和细胞中Akt和P-Akt的表达水平。 结果 糖尿病肾病组和高糖组miR-92b表达水平明显低于对照组(P<0.05)，而P-Akt/Akt表达水平明显高于对照组(P<0.05)。将信号通路PI3K/Akt抑制剂LY294002加入到高糖诱导的糖尿病肾病细胞模型中，信号通路PI3K/Akt被抑制的同时，miR-92b表达显著升高(P<0.05)。结论 糖尿病肾病时，miR-92b与PI3K/Akt的表达呈负相关。

    [关键词] 糖尿病肾病;miR-92b;PI3K;Akt;LY294002

    [中图分类号] R587.2;R364.3? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-9701(2021)30-0044-05

    [Abstract] Objective To explore the relationship between miR-92b and PI3K/Akt signaling pathway in diabetic nephropathy. Methods Twelve 8-week-old male C57BL/6 mice were randomly divided into a normal control group and a diabetic nephropathy group. The diabetic nephropathy mouse model was established using sodium citrate. 12 bottles of human renal tubular epithelial HK-2 cells were selected and randomly divided into high glucose group ......