湿热-痰结-瘀毒型小鼠肠癌模型的建立(1)
〔摘要〕 目的 建立一种湿热-痰结-瘀毒的大肠癌动物模型，为中医药预防大肠癌实验的多方位多角度开展提供模型保障。方法 将60只雄性小鼠随机分为实验A组、B组及C组，每组20只。A组腹腔注射氧化偶氮甲烷(Azoxymethane， AOM) 12.5 mg/kg;B組腹腔注射AOM 10 mg/kg ;C组一直为空白组，腹腔注射等量生理盐水，均1次。随后A、B两组行3个循环周期，自由饮用2.5%葡聚糖硫酸钠5 d+自由饮用无菌用水16 d为1个循环周期;C组自由饮用无菌用水，3组均采用高脂颗粒饲料喂养。造模结束后，断颈处死小鼠观察其瘤体组织变化。结果 与C组比较，A组和B组的造模前后的体质量差异有统计学意义(P<0.05)，B组体质量变化大于A组(P<0.05);A组腺癌成癌率较高，B组腺瘤成瘤率较高，差异均有统计学意义(P<0.05);造模过程中A组死亡率大于C组，差异有统计学意义(P<0.05)。组织病理学显示A组腺癌细胞排列紊乱，乳头内间质少;B组腺瘤细胞排列呈大小不一的管状结构，腺上皮细胞数目增多，核细长，呈不同程度的异型性。结论 AOM 10 mg/kg剂量组适合腺瘤(痰结)模型，AOM 12.5 mg/kg更适合恶性肿瘤(瘀毒)模型。
〔关键词〕 结直肠癌;中医证候模型;氧化偶氮甲烷;高脂颗粒饲料
〔中图分类号〕R273 〔文献标志码〕A 〔文章编号〕doi：10.3969/j.issn.1674-070X.2020.01.009
〔Abstract〕 Objective To establish an animal model of colorectal cancer with damp-heat， phlegm-stagnation， stasis-toxin， and to provide model guarantee for the experiment of preventing colorectal cancer by traditional Chinese medicine (TCM). Methods A total of 60 male mice were divided into an experimental group A (n = 20)， a group B (n = 20)， and a group C (n = 20). Group A was given intraperitoneal injection for AOM 12.5 mg/kg. Group B was given intraperitoneal injection for AOM 10 mg/kg. Group C was the blank group and was given intraperitoneal injection for the same amount of normal saline， all for once. Subsequently， Group A and B performed 3 cycles， and free drinking of 2.5% dextran sodium sulfate for 5 d + free drinking of sterile water for 16 days was a cycle; Group C had free drinking of sterile water for 3 cycles， and all of the 3 groups had high-fat pellet feed. After the modeling was completed， the mice were sacrificed by breaking neck to observe their tumor tissue changes. Results Compared with the group C， the differences in body weight before and after modeling were statistically significant in group A and group B (P<0.05). The change in body weight in group B was greater than that in group A (P<0.05); The adenocarcinoma formation rate of group A was higher， and the adenocarcinoma formation rate of group B was higher. The differences were statistically significant (P<0.05). The mortality of group A was greater than that of group C during the modeling process， and the difference was statistically significant (P<0.05). Histopathology showed disordered arrangement of adenocarcinoma cells in group A with less interstitial nipples; adenoma cells in group B had tubular structures of different sizes， increased number of glandular epithelial cells， slender nuclei， and different degrees of atypical. Conclusion AOM 10 mg/kg dose group is suitable for adenoma (phlegm-stagnation) model， and AOM 12.5 mg/kg is more suitable for malignant tumor (stasis-toxin) model., 百拇医药(曹文 周小青)
〔关键词〕 结直肠癌;中医证候模型;氧化偶氮甲烷;高脂颗粒饲料
〔中图分类号〕R273 〔文献标志码〕A 〔文章编号〕doi：10.3969/j.issn.1674-070X.2020.01.009
〔Abstract〕 Objective To establish an animal model of colorectal cancer with damp-heat， phlegm-stagnation， stasis-toxin， and to provide model guarantee for the experiment of preventing colorectal cancer by traditional Chinese medicine (TCM). Methods A total of 60 male mice were divided into an experimental group A (n = 20)， a group B (n = 20)， and a group C (n = 20). Group A was given intraperitoneal injection for AOM 12.5 mg/kg. Group B was given intraperitoneal injection for AOM 10 mg/kg. Group C was the blank group and was given intraperitoneal injection for the same amount of normal saline， all for once. Subsequently， Group A and B performed 3 cycles， and free drinking of 2.5% dextran sodium sulfate for 5 d + free drinking of sterile water for 16 days was a cycle; Group C had free drinking of sterile water for 3 cycles， and all of the 3 groups had high-fat pellet feed. After the modeling was completed， the mice were sacrificed by breaking neck to observe their tumor tissue changes. Results Compared with the group C， the differences in body weight before and after modeling were statistically significant in group A and group B (P<0.05). The change in body weight in group B was greater than that in group A (P<0.05); The adenocarcinoma formation rate of group A was higher， and the adenocarcinoma formation rate of group B was higher. The differences were statistically significant (P<0.05). The mortality of group A was greater than that of group C during the modeling process， and the difference was statistically significant (P<0.05). Histopathology showed disordered arrangement of adenocarcinoma cells in group A with less interstitial nipples; adenoma cells in group B had tubular structures of different sizes， increased number of glandular epithelial cells， slender nuclei， and different degrees of atypical. Conclusion AOM 10 mg/kg dose group is suitable for adenoma (phlegm-stagnation) model， and AOM 12.5 mg/kg is more suitable for malignant tumor (stasis-toxin) model., 百拇医药(曹文 周小青)
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