甲强龙对大鼠急性脊髓损伤后血中丙二醛的影响(1)
[摘要] 目的 探討大剂量甲基强的松龙(methylprednisolone，MP)对急性脊髓损伤(acute spinal cord injury，ASCI)后大鼠血清中丙二醛(Malonic Dialdehyde，MDA)的影响。方法 该试验于2015年7月—2016年8月进行。90只雄性SD大鼠，随机分成A，B，C 3组，每组30只。A 组单纯全椎板切除，不损伤脊髓；B，C组采用Allen's法制作T10脊髓损伤模型，C组首计量以30 mg/kg甲基强的松龙尾静脉注射，余计量以5.4 mg/(kg·h)，每4 h静脉给药1次，24 h给完，A、B组以生理盐水尾静脉注射，计量同C组。术后24 h，48 h对3组大鼠后肢运动功能行BBB评分；损伤脊髓病理观察。应用ELISA方法检测各组中血清MDA含量。结果 BBB评分A组中24 h与48 h亚组分别为(19.60±0.97)，(19.90±0.74)；B组中24 h与48 h亚组分别为(0.80±0.63)，(1.00±0.94)；C组中24 h与48 h亚组分别为(2.40±1.07)，(2.60±0.97)。经比较C组与B组，在运动功能恢复上差异有统计学意义(P <0.01)，C组明显优于B组。血清MD含量变化不同时间点血清中丙二醛含量浓度(ng/mL)，A组中24 h与48 h亚组分别为(2.45±0.23)，(2.46±0.26)；B组中24 h与48 h亚组分别为(6.25±0.59)，(6.74±0.31)；C组中24 h与48 h亚组分别为(4.53±0.43)，(4.44±0.50)。B组血清中MDA含量较A和C组明显增高(P<0.01)，C组血清中MDA含量较A组明显增高(P<0.05)。C组大鼠在损伤后24 h，48 h后BBB评分高于B组，差异有统计学意义(P<0.01)。B，C组血清中的MDA浓度较A组均明显升高，而C组血清的MDA浓度显著低于B组，差异有统计学意义(P<0.01)。 结论 大鼠急性脊髓损伤后血清中MDA显著升高，早期应用大剂量MP后血清MDA明细降低，提示早期大剂量的MP治疗对脊髓急性损伤具有明显的保护作用。
[关键词] 脊髓损伤；甲基强的松龙；丙二醛；大鼠
[中图分类号] R683.2 [文献标识码] A [文章编号] 1674-0742(2017)01(c)-0059-04
Effect of Urbason on the Blood Malonaldehyde of Rats after the Acute Spinal Cord Injury
HU Xue-feng， GUO Peng-nian， JIANG Ji-zhe， LIU Rui
Department of Orthopaedics， First Affiliated Hospital of Baotou Medical College， Baotou， Inner Mongolia， 014010 China
[Abstract] Objective To study the effect of large-dose urbason on the blood malonaldehyde？of rats after the acute spinal cord injury. Methods 90 male SD rats from July， 2015 to August， 2016 were randomly divided into three groups with 30 cases in each， the group A were given the laminectomy without spinal marrow injury， the group B and group C made the T10 spinal marrow injury model by the Allen's method， the group C were given the intravenous injection of 30 mg/kg methylprednisolone at first and then were given the intravenous administration of 5.4 mg/(kg·h) once per 4 h， and the administration was complete within 24 h， and the group A and group B were given the intravenous injection of normal saline and the amount was the same as that in the group C， and the posterior limb motion function of the three groups at 24 h and 48 h after operation were given the BBB score， and the serum MDA content in each group was tested by the ELISA method. Results The BBB scores in the 24 h and 48 h subgroups A， B and C were respectively(19.60±0.97)，(19.90±0.74)，(0.80±0.63)，(1.00±0.94) and (2.40±1.07)，(2.60±0.97)， and the difference in the motion function recovery between the group C and group B had statistical significance(P<0.01)， and the malonaldehyde content concentrations in serum in the 24 h and 48 h subgroups A， B and C were respectively(2.45±0.23)，(2.46±0.26)，(6.25±0.59)，(6.74±0.31) and (4.53±0.43)，(4.44±0.50)， the MDA content in the group B obviously increased compared with that in the group A and group C(P<0.01)， and the MDA content in the group C obviously increased compared with that in the group A(P<0.05)， and the BBB scores at 24 h and 48 h after injury in the group C were higher than those in the group B， and the difference had statistical significance(P<0.01)， and the MDA concentration in serum in the group C obviously increased compared with that in the group A， and the MDA concentration in serum in the group C was obviously lower than that in the group B， and the difference had statistical significance(P<0.01). Conclusion The MDA in serum of rats after the acute spinal cord injury obviously increases， and the early application of large-dose MP can obviously reduce the serum MDA， which reminds that the early large-dose MP treatment has an obvious protective effect on the injury of the acute spinal cord injury., 百拇医药(胡雪峰 郭鹏年 姜霁哲 刘瑞)
[关键词] 脊髓损伤；甲基强的松龙；丙二醛；大鼠
[中图分类号] R683.2 [文献标识码] A [文章编号] 1674-0742(2017)01(c)-0059-04
Effect of Urbason on the Blood Malonaldehyde of Rats after the Acute Spinal Cord Injury
HU Xue-feng， GUO Peng-nian， JIANG Ji-zhe， LIU Rui
Department of Orthopaedics， First Affiliated Hospital of Baotou Medical College， Baotou， Inner Mongolia， 014010 China
[Abstract] Objective To study the effect of large-dose urbason on the blood malonaldehyde？of rats after the acute spinal cord injury. Methods 90 male SD rats from July， 2015 to August， 2016 were randomly divided into three groups with 30 cases in each， the group A were given the laminectomy without spinal marrow injury， the group B and group C made the T10 spinal marrow injury model by the Allen's method， the group C were given the intravenous injection of 30 mg/kg methylprednisolone at first and then were given the intravenous administration of 5.4 mg/(kg·h) once per 4 h， and the administration was complete within 24 h， and the group A and group B were given the intravenous injection of normal saline and the amount was the same as that in the group C， and the posterior limb motion function of the three groups at 24 h and 48 h after operation were given the BBB score， and the serum MDA content in each group was tested by the ELISA method. Results The BBB scores in the 24 h and 48 h subgroups A， B and C were respectively(19.60±0.97)，(19.90±0.74)，(0.80±0.63)，(1.00±0.94) and (2.40±1.07)，(2.60±0.97)， and the difference in the motion function recovery between the group C and group B had statistical significance(P<0.01)， and the malonaldehyde content concentrations in serum in the 24 h and 48 h subgroups A， B and C were respectively(2.45±0.23)，(2.46±0.26)，(6.25±0.59)，(6.74±0.31) and (4.53±0.43)，(4.44±0.50)， the MDA content in the group B obviously increased compared with that in the group A and group C(P<0.01)， and the MDA content in the group C obviously increased compared with that in the group A(P<0.05)， and the BBB scores at 24 h and 48 h after injury in the group C were higher than those in the group B， and the difference had statistical significance(P<0.01)， and the MDA concentration in serum in the group C obviously increased compared with that in the group A， and the MDA concentration in serum in the group C was obviously lower than that in the group B， and the difference had statistical significance(P<0.01). Conclusion The MDA in serum of rats after the acute spinal cord injury obviously increases， and the early application of large-dose MP can obviously reduce the serum MDA， which reminds that the early large-dose MP treatment has an obvious protective effect on the injury of the acute spinal cord injury., 百拇医药(胡雪峰 郭鹏年 姜霁哲 刘瑞)