不同免疫检验方法检测乙肝病毒感染血清标志物的对比评价(1)
[摘要] 目的 探讨不同免疫检验方法检测乙肝病毒感染血清标志物的效果差异性。方法 方便选择2016年8月—2017年8月间该院收治的80例乙肝病毒感染患者实施观察研究，对所有患者均实施电化学发光法、酶联免疫吸附法检测，分析总结两种免疫检测方式对患者血清标志物(表面抗原HBsAg、表面抗体HBsAb、e抗原HBeAg、e抗体HBeAb、核心抗体HBcAb)检测结果差异性。结果 电化学发光法检测HBsAg、HBeAg、HBeAb阳性率70.0%、31.3%、28.8%较酶联免疫吸附法52.5%、17.5%、15.0%相比明显升高(P=0.023、0.043、0.035)。电化学发光法检测HBsAb、HBcAb阳性率17.5%、72.5%较酶联免疫吸附法15.0%、70.0%相比差异无统计学意义(P=0.668、0.727)。实施电化学发光法对乙肝病毒感染诊断准确率97.5%较酶联免疫吸附法检测准确率83.8%明显较高(P=0.003)。结论 免疫检验方法中电化学发光法检测乙肝病毒感染血清标志物效果优于酶联免疫吸附法。
[关键词] 免疫检验；电化学发光法；酶联免疫吸附法；乙肝病毒感染；血清标志物
[中图分类号] R5 [文献标识码] A [文章编号] 1674-0742(2017)12(c)-0035-03
[Abstract] Objective This paper tries to investigate the difference clinic effect of different immunoassay methods in detecting serum markers of hepatitis B virus infection. Methods 80 patients with hepatitis B virus infection were convenient selected from August 2016 to August 2017 in this department. All patients were examined by electrochemiluminescence and enzyme-linked immunosorbent assay (ELISA)， and the results of two immunoassay methods Patient serum markers (surface antigen HBsAg， surface antibody HBsAb， e-antigen HBeAg， e-antibody HBeAb， core antibody HBcAb) test results differences. Results The positive rates of HBsAg， HBeAg and HBeAb by electrochemiluminescence were 70.0%， 31.3% and 28.8%， respectively， which were significantly higher than those by enzyme-linked immunosorbent assay of 52.5%， 17.5% and 15.0% (P=0.023， 0.043， 0.035). The positive rate of HBsAb and HBcAb detected by electrochemiluminescence was 17.5%， 72.5% compared with 15.0% and 70.0% of enzyme-linked immunosorbent assay (P=0.668， 0.727). The diagnostic accuracy of electrochemiluminescence assay for hepatitis B virus infection was 97.5%， which was significantly higher than that of enzyme-linked immunosorbent assay (83.8%) (P=0.003). Conclusion Immunoassay method in the detection of hepatitis B virus infection by electrochemiluminescence method serum markers better than enzyme-linked immunosorbent assay.
[Key words] Immunoassay； Electrochemiluminescence； Enzyme-linked immunosorbent assay； Hepatitis B virus infection； Serum markers
乙肝病毒感染是引起引發乙型病毒性肝炎、肝硬化、肝癌的重要因素，严重威胁患者生命安全。加强对乙肝病毒感染的早期诊断，及时确定患者感染状况是防治乙型病毒性肝炎的关键。乙肝病毒感染血清标志物检测是了解乙肝病毒感染后机体免疫过程的重要指标，也是当前临床了解乙肝病毒感染的重要检测方式[1]。目前临床检测乙肝病毒感染血清标志物的方式主要分为电化学发光法和酶联免疫吸附法。但临床对于上述两种检查方式的选择仍存在一定争议。基于以上原因，对2016年8月—2017年8月间该院收治的80例乙肝病毒感染患者均实施了电化学发光法和酶联免疫吸附法检测患者血清标志物状况，现报道如下。
1 资料与方法
1.1 一般资料
方便选择该院收治的80例乙肝病毒感染患者实施观察研究。患者中含有男48例，女32例；患者年龄跨度20～76岁，平均年龄(45.3±9.5)。纳入标准：实施临床检测、病毒检测等确诊为乙肝病毒感染患者；自愿签署知情同意书患者；研究过程经医院伦理委员会批准。排除标准：其他病毒性感染患者；恶性肿瘤性疾病、严重心肺肾功能损伤患者；妊娠、哺乳状态患者；其他类型肝炎及肝病患者；精神障碍患者；不配合检查患者。, http://www.100md.com(张轶)
[关键词] 免疫检验；电化学发光法；酶联免疫吸附法；乙肝病毒感染；血清标志物
[中图分类号] R5 [文献标识码] A [文章编号] 1674-0742(2017)12(c)-0035-03
[Abstract] Objective This paper tries to investigate the difference clinic effect of different immunoassay methods in detecting serum markers of hepatitis B virus infection. Methods 80 patients with hepatitis B virus infection were convenient selected from August 2016 to August 2017 in this department. All patients were examined by electrochemiluminescence and enzyme-linked immunosorbent assay (ELISA)， and the results of two immunoassay methods Patient serum markers (surface antigen HBsAg， surface antibody HBsAb， e-antigen HBeAg， e-antibody HBeAb， core antibody HBcAb) test results differences. Results The positive rates of HBsAg， HBeAg and HBeAb by electrochemiluminescence were 70.0%， 31.3% and 28.8%， respectively， which were significantly higher than those by enzyme-linked immunosorbent assay of 52.5%， 17.5% and 15.0% (P=0.023， 0.043， 0.035). The positive rate of HBsAb and HBcAb detected by electrochemiluminescence was 17.5%， 72.5% compared with 15.0% and 70.0% of enzyme-linked immunosorbent assay (P=0.668， 0.727). The diagnostic accuracy of electrochemiluminescence assay for hepatitis B virus infection was 97.5%， which was significantly higher than that of enzyme-linked immunosorbent assay (83.8%) (P=0.003). Conclusion Immunoassay method in the detection of hepatitis B virus infection by electrochemiluminescence method serum markers better than enzyme-linked immunosorbent assay.
[Key words] Immunoassay； Electrochemiluminescence； Enzyme-linked immunosorbent assay； Hepatitis B virus infection； Serum markers
乙肝病毒感染是引起引發乙型病毒性肝炎、肝硬化、肝癌的重要因素，严重威胁患者生命安全。加强对乙肝病毒感染的早期诊断，及时确定患者感染状况是防治乙型病毒性肝炎的关键。乙肝病毒感染血清标志物检测是了解乙肝病毒感染后机体免疫过程的重要指标，也是当前临床了解乙肝病毒感染的重要检测方式[1]。目前临床检测乙肝病毒感染血清标志物的方式主要分为电化学发光法和酶联免疫吸附法。但临床对于上述两种检查方式的选择仍存在一定争议。基于以上原因，对2016年8月—2017年8月间该院收治的80例乙肝病毒感染患者均实施了电化学发光法和酶联免疫吸附法检测患者血清标志物状况，现报道如下。
1 资料与方法
1.1 一般资料
方便选择该院收治的80例乙肝病毒感染患者实施观察研究。患者中含有男48例，女32例；患者年龄跨度20～76岁，平均年龄(45.3±9.5)。纳入标准：实施临床检测、病毒检测等确诊为乙肝病毒感染患者；自愿签署知情同意书患者；研究过程经医院伦理委员会批准。排除标准：其他病毒性感染患者；恶性肿瘤性疾病、严重心肺肾功能损伤患者；妊娠、哺乳状态患者；其他类型肝炎及肝病患者；精神障碍患者；不配合检查患者。, http://www.100md.com(张轶)