2016年贺州市HIV抗体筛查阳性的复检与确证结果分析(1)
[摘要] 目的 分析該市2016年HIV抗体筛查阳性的复检与确证结果，为艾滋病的防治工作提供参考信息。方法 选取2016年1—12月该市各艾滋病筛查实验室送检的初筛为阳性的581份标本，运用第四代酶联免疫吸附试验和胶体硒试验对HIV抗体初筛阳性标本进行复检，运用免疫印迹法对阳性结果进行确证试验，并分析确证实验结果、确证阳性标本的带型分布以及不确定标本的带型分布与随访情况。结果 581份HIV抗体筛查阳性标本经复检，需要进行确证的标本共478份，去掉32份既往阳性，另外446份标本的确证结果显示，HIV抗体呈阳性391份，阳性率为87.7%；HIV抗体呈阴性18份，阴性率为4.0%；不确定37份，占比8.3%。疾控中心的HIV抗体阳性率最高(95.6%)，其后依次是医疗机构(88.4%)、采供血机构(54.2%)。391份确证阳性标本中，条带gp160、gp120、gp41、p24的出现率依次为100.0%、100.0%、99.7%、98.5%，出现率最低的是p39(26.1%)。不确定标本共6种带型，以gp160+gp120+gp41的出现率最高(为37.9%)，gp160+gp24+p17的出现率最低(为2.7%)；成功随访28例，其中转阳9例，转阳率为32.2%。结论 HIV抗体筛查阳性难以避免，应及时进行复检，并对复检阳性结果进行确证，确证结果不确定的标本应进行随访检测。
[关键词] HIV抗体；筛查；阳性；复检；确证
[中图分类号] R4 [文献标识码] A [文章编号] 1674-0742(2018)03(c)-0165-03
Analysis of Reexamination and Confirmation Results of HIV Antibody Screening Specimens in Hezhou in 2016
HUANG Dan-mei
Hezhou Disease Prevention and Control Center， Hezhou， Guangxi， 542899 China
[Abstract] Objective To analyze the reexamination and confirmation results of HIV positive antibody screening in Hezhou in 2016 thus providing the reference for the prevention and treatment work. Methods 581 pieces of positive specimens by the initial screening submitted to the laboratory from January 2016 to December 2016 were selected， and the HIV antibody screening positive specimens were reexamined by the four-generation enzyme linked immunosorbent assay and colloid selenium test， and the positive results were confirmed and tested by the immunoblotting， and the confirmation experimental results， confirmation positive specimens typing distribution and uncertain specimens typing distribution and follow-up were analyzed. Results Of 581 pieces of HIV antibody screening positive specimens， there were 478 pieces of specimens needing confirmation， except 32 cases of past positive specimens， of the other 446 pieces of specimens， there were 391 pieces of positive HIV antibody， the positive rate was 87.7%， and 18 pieces of negative HIV antibody， and the negative rate was 4.0%， and 37 pieces of uncertain specimens， accounting for 8.3%， and in the disease control center， the HIV antibody positive rate was the highest， accounting for 95.6%， the medical institution was the second， accounting for 88.4%， and then the blood supply and collection institutions， accounting for 54.2%， of 391 pieces of positive specimens， the occurrence rate of band gp160，gp120，gp41，p24 was respectively 100.0%，100.0%，99.7%，98.5%， and the occurrence rate of p39 was the lowest， accounting for 26.1%， and there were 6 types of uncertain specimens， the occurrence rate of gp160+gp120+gp41 was the highest， accounting for 37.9%， and the occurrence rate of gp160+gp24+p17 was the lowest， accounting for 2.7%， and 28 cases were successfully followed up， including 9 cases converted to positive， and the rate of conversion to positive was 32.2%. Conclusion The HIV antibody screening positive specimens can’t be avoided， and we should conduct the reexamination in time， and confirm the positive results， and the follow up the specimens whose confirmation results are uncertain., 百拇医药(黄丹梅)
[关键词] HIV抗体；筛查；阳性；复检；确证
[中图分类号] R4 [文献标识码] A [文章编号] 1674-0742(2018)03(c)-0165-03
Analysis of Reexamination and Confirmation Results of HIV Antibody Screening Specimens in Hezhou in 2016
HUANG Dan-mei
Hezhou Disease Prevention and Control Center， Hezhou， Guangxi， 542899 China
[Abstract] Objective To analyze the reexamination and confirmation results of HIV positive antibody screening in Hezhou in 2016 thus providing the reference for the prevention and treatment work. Methods 581 pieces of positive specimens by the initial screening submitted to the laboratory from January 2016 to December 2016 were selected， and the HIV antibody screening positive specimens were reexamined by the four-generation enzyme linked immunosorbent assay and colloid selenium test， and the positive results were confirmed and tested by the immunoblotting， and the confirmation experimental results， confirmation positive specimens typing distribution and uncertain specimens typing distribution and follow-up were analyzed. Results Of 581 pieces of HIV antibody screening positive specimens， there were 478 pieces of specimens needing confirmation， except 32 cases of past positive specimens， of the other 446 pieces of specimens， there were 391 pieces of positive HIV antibody， the positive rate was 87.7%， and 18 pieces of negative HIV antibody， and the negative rate was 4.0%， and 37 pieces of uncertain specimens， accounting for 8.3%， and in the disease control center， the HIV antibody positive rate was the highest， accounting for 95.6%， the medical institution was the second， accounting for 88.4%， and then the blood supply and collection institutions， accounting for 54.2%， of 391 pieces of positive specimens， the occurrence rate of band gp160，gp120，gp41，p24 was respectively 100.0%，100.0%，99.7%，98.5%， and the occurrence rate of p39 was the lowest， accounting for 26.1%， and there were 6 types of uncertain specimens， the occurrence rate of gp160+gp120+gp41 was the highest， accounting for 37.9%， and the occurrence rate of gp160+gp24+p17 was the lowest， accounting for 2.7%， and 28 cases were successfully followed up， including 9 cases converted to positive， and the rate of conversion to positive was 32.2%. Conclusion The HIV antibody screening positive specimens can’t be avoided， and we should conduct the reexamination in time， and confirm the positive results， and the follow up the specimens whose confirmation results are uncertain., 百拇医药(黄丹梅)