人端粒酶TERT蛋白和P16蛋白在宫颈病变中的表达研究(1)
[摘要] 目的 探討人端粒酶TERT蛋白与P16蛋白免疫组化检测在宫颈上皮内瘤变(CIN)诊断中的应用价值。 方法 方便选取2016年2月—2018年2月于该院就诊并经病理确诊的91例CIN患者及29例宫颈癌(Ca)作为研究对象，另选取同期健康女性20名作为对照组。91例CIN患者经病理证实为CIN I级20例，CIN II级38例，CIN III级33例。所有患者TCT、HPV检查阴性，采集标本组织检测hTERT及P16表达情况，总结hTERT、P16在宫颈病变中的表达特点及临床检测价值。结果 对照组、CIN I、II、III级、Ca患者hTERT蛋白阳性率分别为5.00%、15.00%、60.53%、72.73%、96.55%，P16表达阳性率分别为10.00%、15.00%、73.68%、78.79%、100.00%。Ca、CIN II、III级患者hTERT蛋白、P16阳性率明显高于CIN I级患者与对照组，差异有统计学意义(P<0.05)，并且Ca患者hTERT蛋白、P16阳性率明显高于其他组，差异有统计学意义(P<0.05)。 结论 宫颈癌、CINIII、CINII组织中P16、端粒酶TERT蛋白表达阳性率均异常升高，表明其与宫颈癌的发生发展具有密切联系，可作为CIN分级与宫颈癌诊断、治疗评估的重要指标。
[关键词] 人TERT蛋白;P16蛋白;免疫组化检测;宫颈上皮内瘤变;宫颈癌
[中图分类号] R737 [文献标识码] A [文章编号] 1674-0742(2019)05(a)-0037-03
[Abstract] Objective To investigate the value of human telomerase TERT protein and P16 protein immunohistochemical detection in the diagnosis of cervical intraepithelial neoplasia (CIN). Methods A total of 91 CIN patients and 29 cases of cervical carcinoma diagnosed by pathology in our hospital from February 2016 to February 2018 were selected as the research objects， and 20 healthy women in the same period were selected as the control group. There were 91 CIN patients who were pathologically confirmed as CIN I in 20 cases， CIN II in 38 cases and CIN III in 33 cases. All patients examined the TCT and HPV. Specimens were collected to detect the expression of hTERT and P16. And the expression characteristics and clinical detection value of hTERT and P16 in cervical lesions were summarized. Results The positive rates of hTERT protein in control group of CIN I， II， III and Ca patients were 5.00%， 15.00%， 60.53%， 72.73%， 96.55%， and the positive rates of P16 expression were 10.00%， 15.00%， 73.68%， 78.79% and 100.00%， respectively. The positive rates of hTERT protein and P16 in Ca， CIN II and III patients were significantly higher than those in CIN I and control group (P<0.05)， and the positive rates of hTERT protein and P16 in Ca patients were significantly higher than those in other groups (P<0.05)， with statistically significant differences. Conclusion The positive expression rates of P16 and telomerase TERT protein in cervical cancer， CINIII and CINII tissues were all significantly increased， indicating that they are closely related to the occurrence and development of cervical cancer and can be used as an important indicator for CIN classification， diagnosis and treatment evaluation of cervical cancer., http://www.100md.com(刘东涌)
[关键词] 人TERT蛋白;P16蛋白;免疫组化检测;宫颈上皮内瘤变;宫颈癌
[中图分类号] R737 [文献标识码] A [文章编号] 1674-0742(2019)05(a)-0037-03
[Abstract] Objective To investigate the value of human telomerase TERT protein and P16 protein immunohistochemical detection in the diagnosis of cervical intraepithelial neoplasia (CIN). Methods A total of 91 CIN patients and 29 cases of cervical carcinoma diagnosed by pathology in our hospital from February 2016 to February 2018 were selected as the research objects， and 20 healthy women in the same period were selected as the control group. There were 91 CIN patients who were pathologically confirmed as CIN I in 20 cases， CIN II in 38 cases and CIN III in 33 cases. All patients examined the TCT and HPV. Specimens were collected to detect the expression of hTERT and P16. And the expression characteristics and clinical detection value of hTERT and P16 in cervical lesions were summarized. Results The positive rates of hTERT protein in control group of CIN I， II， III and Ca patients were 5.00%， 15.00%， 60.53%， 72.73%， 96.55%， and the positive rates of P16 expression were 10.00%， 15.00%， 73.68%， 78.79% and 100.00%， respectively. The positive rates of hTERT protein and P16 in Ca， CIN II and III patients were significantly higher than those in CIN I and control group (P<0.05)， and the positive rates of hTERT protein and P16 in Ca patients were significantly higher than those in other groups (P<0.05)， with statistically significant differences. Conclusion The positive expression rates of P16 and telomerase TERT protein in cervical cancer， CINIII and CINII tissues were all significantly increased， indicating that they are closely related to the occurrence and development of cervical cancer and can be used as an important indicator for CIN classification， diagnosis and treatment evaluation of cervical cancer., http://www.100md.com(刘东涌)