趋化因子受体CXCR3对神经病理性疼痛的行为学影响(1)
[摘要] 目的 探討分析趋化因子受体CXCR3在神经病理性疼痛中的表达变化与调控过程中发挥的作用。 方法 实验组将野生型C57BL/6J雄性成年小鼠构建坐骨神经慢性压迫(chronic constriction injury， CCI)模型，对照组坐骨神经组织仅暴露不结扎，通过Western Blot方法，检测CCI处理后CXCR3的mRNA表达的变化;通过检测机械缩足反射阈值(MWT)和热刺激缩足阈值(TWL)，观察实施CXCR3基因敲除小鼠(N组)，以及同源野生型C57BL/6J小鼠(C组)在进行CCI后的疼痛行为。归纳分析趋化因子受体CXCR3对神经病理性疼痛调控干预过程所造成的影响作用，并且对比分析相关实验指标。 结果 在CCI处理后1 d、3 d、7 d，及14 d，C组的机械缩足反射阈值(6.50±0.24)、(5.24±0.22)、(4.85±0.19)、(3.43±0.23)低于N组(14.22±0.52)、(13.75±0.41)、(12.34±0.25)、(11.41±0.25)，组间差异有统计学意义(t=42.627、t=57.836、t=75.430、t=74.285，P<0.05)。 结论 在神经病理性疼痛形成过程中，CXCR3基因表达增加，能加快神经损伤后机械痛感现象的形成过程。
[关键词] 趋化因子受体CXCR3;神经病理性疼痛;机械缩足反射阈值;热刺激缩足阈值
[中图分类号] R745 [文献标识码] A [文章编号] 1674-0742(2020)01(b)-0032-03
Behavioral Effects of Chemokine Receptor CXCR3 on Neuropathic Pain
ZHAO Ge1，WU Jun2
1.Jiamusi University， Jiamusi， Heilongjiang Province， 154007 China; 2.Department of Anesthesiology， Heilongjiang Provincial Hospital， Harbin，Heilongjiang Province， 150036 China
[Abstract] Objective To investigate the role of chemokine receptor CXCR3 in the expression and regulation of neuropathic pain. Methods Experimental group will be wild type C57BL / 6J adult male mice build sciatic nerve chronic constriction injury (chronic constriction injury， CCI) model， the control group sciatic nerve tissue exposed ligation， not only by Western Blot method， detection of CCI processing after the change of CXCR3 mRNA expression; the pain behavior of CXCR3 knockout mice (N group) and homologous wild-type C57BL/6J mice (C group) after CCI was observed by detecting mechanical retraction threshold (MWT) and thermal stimulation threshold (TWL). The influence of chemokine receptor CXCR3 on neuropathic pain regulation and intervention process was analyzed， and relevant experimental indexes were compared and analyzed. Results The mechanical indentation reflection threshold(6.50±0.24)，(5.24±0.22)，(4.85±0.19)，(3.43±0.23)，(14.22±0.52)，(13.75±0.41)，(12.34±0.25)，(11.41±0.25) in group c after cci treatment were lower than in group n，the difference between groups was statistically significant(t=42.627，t=57.836，t=75.43，t=74.285，P<0.05). Conclusion During the formation of neuropathic pain， the expression of CXCR3 gene is increased， which can accelerate the formation of mechanical pain after nerve injury.
[Key words] Chemokine receptor CXCR3; Neuropathic pain; Mechanical reflex foot reflex threshold; Thermal stimulation retraction threshold, 百拇医药(赵葛 吴军)
[关键词] 趋化因子受体CXCR3;神经病理性疼痛;机械缩足反射阈值;热刺激缩足阈值
[中图分类号] R745 [文献标识码] A [文章编号] 1674-0742(2020)01(b)-0032-03
Behavioral Effects of Chemokine Receptor CXCR3 on Neuropathic Pain
ZHAO Ge1，WU Jun2
1.Jiamusi University， Jiamusi， Heilongjiang Province， 154007 China; 2.Department of Anesthesiology， Heilongjiang Provincial Hospital， Harbin，Heilongjiang Province， 150036 China
[Abstract] Objective To investigate the role of chemokine receptor CXCR3 in the expression and regulation of neuropathic pain. Methods Experimental group will be wild type C57BL / 6J adult male mice build sciatic nerve chronic constriction injury (chronic constriction injury， CCI) model， the control group sciatic nerve tissue exposed ligation， not only by Western Blot method， detection of CCI processing after the change of CXCR3 mRNA expression; the pain behavior of CXCR3 knockout mice (N group) and homologous wild-type C57BL/6J mice (C group) after CCI was observed by detecting mechanical retraction threshold (MWT) and thermal stimulation threshold (TWL). The influence of chemokine receptor CXCR3 on neuropathic pain regulation and intervention process was analyzed， and relevant experimental indexes were compared and analyzed. Results The mechanical indentation reflection threshold(6.50±0.24)，(5.24±0.22)，(4.85±0.19)，(3.43±0.23)，(14.22±0.52)，(13.75±0.41)，(12.34±0.25)，(11.41±0.25) in group c after cci treatment were lower than in group n，the difference between groups was statistically significant(t=42.627，t=57.836，t=75.43，t=74.285，P<0.05). Conclusion During the formation of neuropathic pain， the expression of CXCR3 gene is increased， which can accelerate the formation of mechanical pain after nerve injury.
[Key words] Chemokine receptor CXCR3; Neuropathic pain; Mechanical reflex foot reflex threshold; Thermal stimulation retraction threshold, 百拇医药(赵葛 吴军)
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