来氟米特对糖尿病肾病大鼠肾脏VEGF表达的影响(1)
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[摘要] 目的:研究氟米特对糖尿病肾病大鼠肾脏血管内皮生长因子(VEGF)表达的影响。方法:将48只清洁级雄性Wistar 大鼠随机分为正常对照组(A组)、模型对照组(B组)、来氟米特组(C组)和氯沙坦组(D组)。分别于糖尿病成模后8、12周处死,观察24 h尿蛋白排泄量、尿素氮(BUN)、肌酐(Scr)、肾脏病理组织学改变、免疫荧光检测肾组织VEGF蛋白的表达,RT-PCR方法检测肾组织VEGF mRNA表达。结果:8、12周末糖尿病模型大鼠(B、C、D组)的Scr、BUN及 24 h尿蛋白较正常对照组(A组)均有显著增高(P<0.01);12周末时B、C、D组大鼠的Scr、BUN及 24 h尿蛋白均较8周时有所增加;来氟米特(LEF)干预组及氯沙坦干预组上述指标均低于B组,差异有统计学意义(P<0.01),但LEF干预组及氯沙坦干预组之间差异无统计学意义。结论:来氟米特和氯沙坦均对糖尿病大鼠肾脏损害有保护作用,其机制可能是通过增加VEGF表达而实现。
[关键词] 来氟米特;糖尿病肾病;血管内皮生长因子;氯沙坦
[中图分类号] R587.2 [文献标识码]A [文章编号]1674-4721(2010)05(a)-018-04
Leflunomide on diabetic nephropathy VEGF expression in rat kidney
ZHAO Dongjia,YU Weimin,LI Rongshan
(Department of Nephrology,the Second Clinical Hospital of Shanxi Medical University, Shanxi Kidney Institute, Shanxi Province ,Taiyuan 030001,China)
[Abstract] Objective: To study the fluoride mitter of diabetic nephropathy rat kidney VEGF expression. Methods: Forty-eight male Wistar rats of clean grade were randomly divided into normal control group (A group), model control group (B group), leflunomide group (C group) and losartan group (D group). Diabetes into the mold, respectively, after 8 weeks, 12 weeks were observed at 24 h urinary protein excretion, blood urea nitrogen (BUN), creatinine (Scr), renal histopathological changes in renal tissue immunofluorescence detection of VEGF protein expression, RT-PCR used to detect VEGF mRNA expression in kidney tissue. Results: 8 weeks, 12 week diabetic rats (B, C, D groups) Scr, BUN, and 24 h urinary protein compared with normal control group (A group) were significantly higher (P<0.01); 12 weekend B, C, D rats in Scr, BUN, and 24 h urinary protein than those of 8 weeks, increased; leflunomide (LEF) in the intervention group and the losartan intervention group were lower than the above-mentioned indicators B group, the difference was significant (P<0.01), but the LEF group and losartan intervention in the intervention group was no significant difference. Conclusion: Losartan both leflunomide and renal damage in diabetic rats has a protective effect, the mechanism may be achieved by increasing the expression of VEGF ......
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