高同型半胱氨酸血症对大鼠股骨骨折骨愈合的影响及干预(1)
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[摘要] 目的:探讨高同型半胱氨酸血症(Hhcy)对大鼠股骨骨折愈合的影响及维生素B12干预后的结果。方法:36只SD大鼠随机分为对照组、Hhcy组、干预组,并制作股骨骨折模型,评价大鼠股骨骨折愈合水平。结果:①Hhcy组大鼠总同型半胱氨酸tHcy(21.56±5.32) μmol/L高于对照组(12.72±3.02) μmol/L(P<0.05),干预组tHcy水平(13.76±6.34) μmol/L与对照组(12.72±3.02) μmol/L比较,差异无统计学意义(P>0.05);②大鼠股骨密度(BMD)Hhcy组(0.175±0.010)g/cm2低于对照组(0.285±0.007) g/cm2 (P<0.05),干预组(0.279±0.015) g/cm2与对照组(0.285±0.007) g/cm2比较,差异无统计学意义(P>0.05)。结论:高同型半胱氨酸血症影响骨折愈合,应用维生素B12可以减轻Hhcy对大鼠骨折愈合的影响。
[关键词] 高同型半胱氨酸血症;B族维生素;骨折;骨密度
[中图分类号] R683.42 [文献标识码]A [文章编号]1674-4721(2010)06(a)-007-02
The influence and intervention of high hyperhomocysteinemia on rat femur bone healing
LIU Xiquan1,WANG Dong2,WANG Pei1
(1.Shanxi Medical University,Shanxi Province,Taiyuan 030001,China;2. Department of Orthopedics,the Second Hopital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China)
[Abstract] Objective: To explore the high hyperhomocysteinemia(Hhcy) on rat femur fractures healing and vitamin B12 intervention results. Methods: Thirty-six SD rats were divided into control group, Hhcy group, the intervention group and made femur fracture model. Evaluated the results of rat femur healing. Results: ①The level of tHcy in Hhcy group(21.56±5.32) μmol/L was higher than that in control group(12.72±3.02) μmol/L (P<0.05),the level of tHcy in intervention group(13.76±6.34) μmol/L compared with the control group (12.72±3.02) μmol/L had no significant difference(P>0.05);②The BMD of femur in Hhcy group (0.175±0.010) g/cm2 was lower than that in control group(0.285±0.007)g/cm2(P<0.05),the BMD of femur in intervention group(0.279±0.015) g/cm2 compared with the control group (0.285±0.007) g/cm2 had no significant difference(P>0.05). Conclusion: High hyperhomocysteinemia effect the fracture healing, and vitamin B12 can reduce adverse effects of Hhcy.
[Key words] High hyperhomocysteinemia;Vitamin B;Fracture;Bone density
股骨干骨折是临床常见骨折,骨折术后不愈合是临床治疗中一种常见的并发症。近年来一些体外细胞培养证实同型半胱氨酸可以影响成骨细胞、破骨细胞的代谢[1-2],这引起了一些学者的重视。本研究利用大鼠饮食所致高同型半胱氨酸血症模型[3],探讨Hhcy对骨折愈合的影响,并研究B族维生素干预对大鼠血浆tHcy的水平以及骨密度的影响。
1 资料与方法
1.1大鼠高同型半胱氨酸血症模型的制作
6月龄、体重200～250 g、健康的SD大鼠(雌雄不限)36只,适应性喂养1周 ......
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