肝素在大鼠外伤性鼓膜穿孔愈合中对转化生长因子-α 的影响(1)
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2010年6月5日
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[摘要] 目的:探讨局部应用肝素在外伤性鼓膜穿孔愈合中对转化生长因子-α(TGF-α)的影响。方法:建立大鼠鼓膜穿孔模型50只,将其随机分成两组,分别用肝素明胶海绵、表皮生长因子(epidermial grouth factor,EGF)明胶海绵黏补,空白对照,观察不同时间点不同组鼓膜愈合情况并取其标本进行免疫组织化学染色,测其转化生长因子-α(TGF-α)的表达水平。结果:肝素组与空白组TGF-α表达有差异(P<0.05),肝素组与EGF组表达基本无差异(P>0.05)。结论:局部应用肝素能促进转化生长因子-α(TGF-α)在鼓膜各层的表达,并延长其表达时间。
[关键词] 肝素;鼓膜穿孔;表皮生长因子;转化生长因子-α
[中图分类号] R764.8 [文献标识码]A [文章编号]1674-4721(2010)06(a)-016-02
The effect of heparin on the TGF-α during the traumatic tympanic membrane perforation in animal models
MIAO Xiaoming,JI Yongjin,LI Qingfeng,GAO Zhujuan,MA Min*
(Department of Otorhinolaryngolog, the Second Affiliated Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China)
[Abstract] Objective: To approach the efficacy of local application heparin on the TGF-α during the treatment of acute traumatic tympanic membrane perforation (TMP) in the animal models. Methods: Developed an animal model of traumatic TMP in the rat and 50 wistar rats were used.They were randomly divided into two groups and treated by heparin combined with gelfoam pledget, EGF combined with gelfoam pledget which was placed over the TMP and blank control separately,then observe the state of the TMP and examined by immunohistochemical method using polyclonal expression level of TGF-α. Results: Statistical difference between treated and control ears in the expression of TGF-α was evident(P<0.05).Statistical difference between heparin and EGF ears in the expression of TGF-α was not evident(P>0.05). Conclusion: Local application heparin can accelerate the expression of healing of TGF-α in acute traumatic TMP in the animal models.
[Key words] Heparin; Hympanic membrane perforation; Epidermal growth factor; Transforming growth factor-α
鼓膜穿孔愈合过程需要多种细胞因子的参与,如表皮生长因子(EGF)、转化生长因子-α(TGF-α)、碱性成纤维细胞生长因子(bFGF)、血小板衍生生长因子(PDGF)、角质形成细胞生长因子(KGF)等。研究发现在创伤愈合的过程中上述生长因子都以与肝素结合的形式存在,不仅可以提高生长因子的生物学效应,而且可以保护其免于被各种理化因素灭活[1-2]。肝素有利于创伤修复已被国内外学者所证实[3-4],表皮生长因子在鼓膜穿孔愈合中的作用也已经被证实[5],肝素在鼓膜穿孔中对转化生长因子-α(TGF-α)的影响至今未见报道。探讨在外伤性鼓膜穿孔愈合过程中肝素对转化生长因子-α(TGF-α)的影响是本研究的重点。
1 资料与方法
1.1 一般资料
Wistar大鼠50只(山西医科大学生理教研室动物中心提供),肝素钠(江苏常州千红生化制药有限公司(12 500 U,2 ml),重组人表皮生长因子(深圳市华生元基因工程发展有限公司,2 000 IU/ml,5ml),兔抗鼠转化生长因子-α(TGF-α),二步法免疫组化检测试剂盒PV6001(武汉博士德) ......
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