阿司匹林对胃肠黏膜损伤ICAM-1表达的临床研究(1)
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[摘要] 目的:探讨阿司匹林对胃肠黏膜损伤ICAM-1表达的临床意义。方法:应用免疫组化检测ICAM-1在大鼠胃黏膜细胞的表达。结果:实验组应用5.021 mg/kg阿司匹林灌胃3 d后,开始出现胃黏膜损伤的表现,损伤指数为(9.07±0.64) mm,14 d损伤达高峰,损伤指数为(23.49±0.57) mm。免疫组化结果显示ICAM-1在用药后3 d开始表达,7 d后逐渐升高,21 d达高峰,28 d及35 d仅有少量表达。实验组用药后与用药前比较ICAM-1表达水平有明显差异;对照组与实验组比较,ICAM-1均明显呈低水平表达。结论:在阿司匹林引起的胃黏膜损伤过程中ICAM-1的表达有一定的临床意义。阿司匹林对胃黏膜有较为明显的损伤,具有一定的危险性。
[关键词] 阿司匹林;胃肠黏膜;ICAM-1表达
[中图分类号] R971+.1 [文献标识码]A [文章编号]1674-4721(2010)06(c)-009-03
Clinical research on aspirin for damage in bronchia mucosal the ICAM-1 express
YAN Fuying
(Zhongshan University Attached First Hospital Huangpu Hospital Area of Guangzhou City, Guangdong Province, Guangzhou510700, China)
[Abstract] Objective: To examine the clinical significance of aspirin to gastrointestinal membrane damage ICAM-1 expression. Methods: Application of immunohistochemistry ICAM-1 testing in the rat stomach mucosa cells expressed. Results: Experimental group applications 5.021 mg/kg aspirin irrigation stomach 3 d, a stomach mucosa injuries and damage index for the (9.07±0.64) mm, 14 d injury peaked and injury index of (23.49±0.57) mm. Immunohistochemistry showed that ICAM-1 in the use of drugs, 3 d beginning to express, 7 d gradually rising and 21 d reached a peak of 28 d and 35 d only a small express. Compared experimental group use of drugs and drug earlier ICAM-1 expressed level, there were significant differences; in contrast with the conthol group, ICAM-1 clearly showed a low voice. Conclusion: Aspirin induce gastric mucosal injury in the course of the expression of ICAM-1 has some clinical significance. Aspirin on gastric mucosal damage is more obvious, a certain degree of risk.
[Key words] Aspirin; Stomach and intestines mucous membrane; ICAM-1 expression
阿司匹林最常见的不良反应是胃肠道刺激,原因之一是由于胃上皮细胞与阿司匹林的直接接触,造成胃壁的损伤,并且用药剂量越大、时间越长,用药所造成的损伤也就越大[1-2]。研究显示,有溃疡出血史的高危患者,阿司匹林与胃黏膜保护剂合用,可以明显减少胃肠刺激症状与出血等副作用。对于这类患者,将胃药与阿司匹林合用,可以保护胃黏膜,比停用阿司匹林的效果要好。
1资料与方法
1.1一般资料
选取140只健康的雄性♂SD裸鼠,平均体重为(220±25) g,随机将其分为两组,对照组70只,实验组70只。两组分为用药前及用药后3、7、14、21、28、35 d每个时间点各10只。
1.2方法
实验前所有大鼠禁食24 h,对照组均给予0.9%NaCl 溶液2 ml/只灌胃。实验组给予阿司匹林5.021 mg/kg灌胃。给药结束后取大鼠胃黏膜样本进行试验检测。①取大鼠胃黏膜组织,冲洗干净,应用显微镜(上海光学仪器厂提供19JC×10)观察糜烂和出血点,胃腺部黏膜组织经4%多聚甲醛固定,常规石蜡包埋、切片行HE染色,光镜(日本索尼公司提供 SONY VF-30CPKS偏震光镜)下观察 ......
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