应用总氧自由基清除能力法检测葡萄籽提取物的抗氧化性(1)
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[摘要] 目的：探讨总氧自由基清除能力法在葡萄籽提取物抗氧化性评价中的应用。方法：热解2，2'-偶氮双(2-脒基丙烷)产生以碳为中心的氧自由基，与4-甲硫基-2-丁酮酸作用产生乙烯，采用气相色谱法检测乙烯含量。在检测体系中添加不同浓度的葡萄籽提取物，从乙烯产量变化指示被测物清除氧自由基的能力。结果：在0.5～10.0 mg/ml浓度范围内，葡萄籽提取物的总氧自由基清除能力存在浓度-反应关系，相关系数为0.864(n=6)。结论：总氧自由基清除能力法可用于葡萄籽提取物抗氧化性的测定，并提出了拓展该方法的思路。
[关键词] 总氧自由基清除能力；葡萄籽提取物；抗氧化性；药品鉴定
[中图分类号] R286.0[文献标识码] C [文章编号] 1674-4721(2011)06(a)-051-02
Determination of the antioxidant of grape seed extract by total oxyradical scavenging capacity assay
LU Qing1, SHEN Feifei2, YE Huaizhuang3
1.The First Affiliated Hospital of Medicine College of Zhejiang University, Zhejiang Province, Hangzhou 310003, China;2. Health Authority of Zhejiang Province, Hangzhou 310009, China; 3.Public Health College of Zhejiang University, Hangzhou 310058, China
[Abstract] Objective: To investigate the application of total oxyradical scavenging capacity (TOSC) assay for evaluating the antioxidant of grape seed extract (GSE). Methods: Peroxyl radicals were generated by pyrolysis of 2,2'-azo-bis (2-methyl-propionamidine)-dihydrochloride (ABAP) at 35℃. Peroxyl radicals could oxidize the substrate α-keto-γ-methiolbutyric acid (KMBA) to ethylene, which was measured by gas chromatography.In the detection system made by adding different concentrations of GSE, indicating changes in ethylene production measured from TOSC. Results: In the range of 0.5-10.0 mg/ml, TOSC of GSE made a linear of concentration-response relationship, the correlation coefficient was 0.864 (n=6). Conclusion: TOSC Assay can be used for GSE antioxidant determination, and proposed to expand the idea of the method.
[Key words] Total Oxyradical scavenging capacity; Grape seed extract; Antioxidant; Drug ldentification
葡萄属于葡萄科(Vitaceae Lindl.)葡萄属(Vitis L.)的果实，为落叶藤本植物。葡萄果实中除果肉外，种籽占4%～6%、果皮占8%。葡萄籽提取物(Grape Seed Extract，GSE)中富含原花青素(proanthocyanidins)，对人体具有抗氧化、清除自由基、改善微循环、调节免疫力、抗突变、抗过敏及抗感染等多种功效[1-2]。
目前已建立了许多抗氧化剂抗氧化性的体外化学检测方法，如FRAP法(ferricion reducing antioxidant power)[3]、TEAC法(trolox equivalent antioxidant capacity)[4]、DPPH法(2,2’-diphenyl-1-picrylhydrazyl radical scavenging capacity)[5]、ORAC法(oxygen radical absorbance capacity)[6]、TRAP法(total peroxyl radical-trapping antioxidant parameter assay)[7-8]、TOSC法(total oxyradical scavenging capacity)[9]等 ......
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