HPLC法测定桑姜感冒片中绿原酸的含量(1)
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[摘要] 目的 建立高效液相色谱(HPLC)法测定桑姜感冒片中绿原酸的方法。 方法 采用HPLC法，以Eclispse XDB C18(4.6 mm×250 mm，5 μm)为色谱分析柱，乙腈-0.4%磷酸溶液(15∶85)为流动相，流速为0.8 mL/min，柱温为30℃，检测波长为326 nm。 结果 绿原酸在0.099～4.990 μg范围内呈良好的线性关系(r = 0.999 8)，平均加样回收率为99.63%，RSD = 0.59%(n = 6)。 结论 该方法操作简单，结果可靠，重现性及加样回收率等试验项目结果均较为理想，可用于桑姜感冒片中绿原酸的质量控制。
[关键词] 桑姜感冒片；高效液相色谱法；绿原酸；含量测定
[中图分类号] R927.2 [文献标识码] A [文章编号] 1674-4721(2012)02(c)-0047-02
Determination contents of caffeotannic acid in Ginger cold tablets with high performance liquid chromatography (HPLC)
SHI Guoju
Pharmaceutical Preparation Section, Shangcai People's Hospital of Henan Province, Shangcai 463800, China
[Abstract] Objective To establish the detecting methods of caffeotannic acid in Ginger cold tablets with the method of high performance liquid chromatography (HPLC). Methods To detect assaying of caffeotannic acid in Ginger cold tablets with HPLC method, and chromatographic column was Eclispse XDB C18 (4.6 mm×250 mm, 5 μm), the moving phase was methyl cyanide and phosphoric acid in 0.4% concentration, the flow rate was 0.8 mL/min, the temperature of column was 30℃, and the wave length was 326 nm. Results Linear correlation of caffeotannic acid was stabile from 0.099 to 4.990 μg (r = 0.999 8), recovery rate of sample was 99.63%, RSD = 0.59% (n = 6). Conclusion HPLC is a simple detecting way, detecting consequence is reliably, repeatability is favourable, and the recovery for the sample is perfect, it could be taken in quality control of caffeotannic acid in Ginger cold tablets.
[Key words] Ginger cold tablets; High performance liquid chromatography (HPLC); Caffeotannic acid; Determination content
桑姜感冒片是由桑叶、菊花、紫苏、连翘、苦杏仁、干姜等药味组成的中成药制剂，具有疏散风热、祛寒止咳之功效，可用于感冒、头痛、咳嗽、咽喉肿痛等病症[1]。方中菊花可疏散风热、清肺止咳，为君药之一，而绿原酸为菊花所含重要抗菌活性成分之一。为控制桑姜感冒片药品质量，本研究特采用HPLC法对桑姜感冒片中绿原酸含量进行测定，为该制剂的质量控制提供了参考性资料。现报道如下：
1 仪器与试药
1.1 仪器
Agilent1200 series高效液相色谱仪；KQ-600GDV型恒温数控超声波清洗器(江苏省昆山超声仪器有限公司)；AL型万分之一电子分析天平(梅特勒-托利多公司)。
1.2 试药
绿原酸对照品(中国药品生物制品检定所，批号110753-200911，供含量测定用)；乙腈、甲醇为色谱纯，磷酸为分析纯，水为娃哈哈纯净水；桑姜感冒片(四川好医生攀西药业有限责任公司，国药准字Z19983141)。
2 方法与结果
2.1 色谱条件
色谱柱：Eclispse XDB C18柱(4 ......
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