血管紧张素原基因M235T突变位点与云南汉族原发性高血压的关系(1)
[摘要] 目的 探讨云南部分汉族原发性高血压(EH)患者与血管紧张素原(AGT)基因M235T突变位点的关系。方法 选择2013年1月～2014年1月年在昆明市第一人民医院心内科住院的行动态血压及相关临床检查确诊为EH的患者122例为EH组，同期入选44例行动态血压排除高血压的患者为对照组。收集EH组和对照组基本临床资料，包括年龄、性别比例、血清总胆固醇、高密度脂蛋白、低密度脂蛋白、三酰甘油、平均左室舒张末径、平均左室收缩末径、左室射血分数等指标，所有患者遵守知情同意的原则，采集被检者静脉血0.5 ml，通过聚合酶链反应技术及基因芯片技术检测AGT基因M235T，同时对样本进行序列测定，比较各组间基因型分布和等位基因的频率分布。 结果 EH组AGT M235T基因型分布与对照组比较，差异无统计学意义(P=0.122)，EH组AGT-TT、AGT-MT和AGT-MM基因型频率分别为43.4%、54.9%、1.6%，对照组分别为31.8%、61.4%、6.8%；T等位基因频率EH组(70.9%)与对照组(62.5%)比较，差异无统计学意义(P=0.145)。 结论 AGT基因M235T基因变异与云南汉族人群EH的发病可能无相关性，AGT基因多态性可能不是EH的危险因素。
[关键词] 原发性高血压；血管紧张素原；基因
[中图分类号] R544.1 [文献标识码] A [文章编号] 1674-4721(2016)03(a)-0041-04
[Abstract] Objective To explore the relationship between essential hypertention(EH) in part of the han nationality in Yunnan and gene M235T mutations of angiotensinogen(AGT). Methods 122 patients，conducted ambulatory blood pressure and related clinical examination and diagnosed as EH in the First People′s Hospital of Kunming Heart Medicine from January 2013 to January 2014 were selected as EH group.44 patients，conducted ambulatory blood pressure and excluded hypertension at the same term，were selected as control group.Basic clinical data included age，sex ratio，serum total cholesterol，high-density lipoprotein，low-density lipoprotein，triglyceride，average left ventricular end-diastolic diameter and average diameter at the end of the left ventricular contraction left ventricular ejection fraction were collected.All patients were abided by principles of informed consent.0.5 ml venous blood of patients were collected and the gene M235T of AGT were tested by using PCR and gene chip technology.The sample were conducted sequence determination to compare the genotype and allele frequency distribution in different groups. Results The difference of AGT M235T genotype distribution between EH group and control group had no statistical significance (P=0.122).The genotype frequency of AGT-TT，AGT-MT and AGT-MM in EH group was respectively 43.4%，54.9% and 1.6%，which was respectively 31.8%，61.4% and 6.8% in control group.The T allele frequency had no obvious difference between EH group(70.9%) and control group(62.5%)(P=0.145). Conclusion It has no relationship between gene M235T mutations of angiotensinogen and essential hypertention in Han nationality of Yunnan crowd.The gene polymorphism of AGT may not be risk factors of essential hypertention.
[Key words] Essential hypertention；Angiotensinogen；Gene
原发性高血压(essential hypertension，EH)是目前发病率最高的一种心血管疾病。现对EH的发病机制仍未研究透彻。但大量研究证实，EH属“复杂疾病”，遗传因素、心理社会因素、体表指数、年龄、膳食等对其发病均有影响，其中20%～40%的血压变化与遗传因素有关，因此筛选高血压候选基因已成为高血压研究的重点之一[1]。众多的EH候选基因及候选位点当中，易感基因最有可能为编码肾素-血管紧张素的基因，其中血管紧张素Ⅱ(AngⅡ)的前体物质是血管紧张素原(angiotensinogen，AGT)，AGT的浓度变化限制了RAS中AngⅡ的转化速度。目前有关AGT基因多态性与EH之间的关系得出的结论各有不同。一方面考虑为研究人群种族遗传及地理环境的差异，其次还与检测方法及检测技术各不同存在很大关系。尽管通过研究不同人群、种族、利用不同的研究技术方法对AGT基因M235T多态性与EH的关系进行研究，同时研究的相关报道也较多，但对云南部分汉族高血压人群的相关研究较少。本研究旨在探讨血管紧张素原基因M235T突变位点与云南汉族人群EH的关系。, 百拇医药(李勇国 王艳飞 马娟)
[关键词] 原发性高血压；血管紧张素原；基因
[中图分类号] R544.1 [文献标识码] A [文章编号] 1674-4721(2016)03(a)-0041-04
[Abstract] Objective To explore the relationship between essential hypertention(EH) in part of the han nationality in Yunnan and gene M235T mutations of angiotensinogen(AGT). Methods 122 patients，conducted ambulatory blood pressure and related clinical examination and diagnosed as EH in the First People′s Hospital of Kunming Heart Medicine from January 2013 to January 2014 were selected as EH group.44 patients，conducted ambulatory blood pressure and excluded hypertension at the same term，were selected as control group.Basic clinical data included age，sex ratio，serum total cholesterol，high-density lipoprotein，low-density lipoprotein，triglyceride，average left ventricular end-diastolic diameter and average diameter at the end of the left ventricular contraction left ventricular ejection fraction were collected.All patients were abided by principles of informed consent.0.5 ml venous blood of patients were collected and the gene M235T of AGT were tested by using PCR and gene chip technology.The sample were conducted sequence determination to compare the genotype and allele frequency distribution in different groups. Results The difference of AGT M235T genotype distribution between EH group and control group had no statistical significance (P=0.122).The genotype frequency of AGT-TT，AGT-MT and AGT-MM in EH group was respectively 43.4%，54.9% and 1.6%，which was respectively 31.8%，61.4% and 6.8% in control group.The T allele frequency had no obvious difference between EH group(70.9%) and control group(62.5%)(P=0.145). Conclusion It has no relationship between gene M235T mutations of angiotensinogen and essential hypertention in Han nationality of Yunnan crowd.The gene polymorphism of AGT may not be risk factors of essential hypertention.
[Key words] Essential hypertention；Angiotensinogen；Gene
原发性高血压(essential hypertension，EH)是目前发病率最高的一种心血管疾病。现对EH的发病机制仍未研究透彻。但大量研究证实，EH属“复杂疾病”，遗传因素、心理社会因素、体表指数、年龄、膳食等对其发病均有影响，其中20%～40%的血压变化与遗传因素有关，因此筛选高血压候选基因已成为高血压研究的重点之一[1]。众多的EH候选基因及候选位点当中，易感基因最有可能为编码肾素-血管紧张素的基因，其中血管紧张素Ⅱ(AngⅡ)的前体物质是血管紧张素原(angiotensinogen，AGT)，AGT的浓度变化限制了RAS中AngⅡ的转化速度。目前有关AGT基因多态性与EH之间的关系得出的结论各有不同。一方面考虑为研究人群种族遗传及地理环境的差异，其次还与检测方法及检测技术各不同存在很大关系。尽管通过研究不同人群、种族、利用不同的研究技术方法对AGT基因M235T多态性与EH的关系进行研究，同时研究的相关报道也较多，但对云南部分汉族高血压人群的相关研究较少。本研究旨在探讨血管紧张素原基因M235T突变位点与云南汉族人群EH的关系。, 百拇医药(李勇国 王艳飞 马娟)