PA对人胃癌细胞放射敏感性的分析(1)
[摘要]目的 观察紫杉醇(PA)对人胃癌细胞株SGC7901放射敏感性的影响。方法 采用MTT法获得PA对SGC7901细胞的半数抑制浓度(IC50)，并以20%的IC50剂量为增敏浓度；克隆形成实验检测单纯照射组与PA(增敏浓度)+照射组在0、2、4、6、8 Gy X线照射后的细胞存活率，并观察细胞周期的改变及细胞形态的变化。结果 PA对SGC7901细胞的IC50为16.11 μg/ml，随着照射剂量的逐渐增加，单纯照射组和PA+照射组细胞存活率呈下降趋势，从4 Gy开始，PA+照射组细胞的存活率低于单纯照射组(P<0.05)；PA+照射组的G2/M期细胞比例高于其他两组，S期细胞比例低于其他两组(P<0.05)。结论 PA对胃癌细胞株SGC7901具有放射增敏作用，其机制可能与诱导细胞凋亡及G2/M期阻滞有关。
[关键词]紫杉醇；人胃癌细胞株；放射增敏
[中图分类号] R739.63 [文献标识码] A [文章编号] 1674-4721(2016)08(a)-0004-03
[Abstract]Objective To observe the effect of paclitaxel (PA) on the radiosensitivity of human gastric cancer cell line SGC7901.Methods MTT assay was employed to obtain the 50% inhibition concentration (IC50) of PA on SGC7901 cells.20% of the IC50 dose was treated as sensitizing concentration.The clonogenic assay was used to analyze the cell viability of radiation group and PA (sensitizer concentration) plus radiation group with X-ray of 0 ......
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[关键词]紫杉醇；人胃癌细胞株；放射增敏
[中图分类号] R739.63 [文献标识码] A [文章编号] 1674-4721(2016)08(a)-0004-03
[Abstract]Objective To observe the effect of paclitaxel (PA) on the radiosensitivity of human gastric cancer cell line SGC7901.Methods MTT assay was employed to obtain the 50% inhibition concentration (IC50) of PA on SGC7901 cells.20% of the IC50 dose was treated as sensitizing concentration.The clonogenic assay was used to analyze the cell viability of radiation group and PA (sensitizer concentration) plus radiation group with X-ray of 0 ......
您现在查看是摘要页，全文长 3392 字符。