低剂量脂多糖对哮喘小鼠T淋巴细胞产生IL—17的影响(1)
[摘要]目的 探讨低剂量脂多糖(LPS)刺激对哮喘小鼠T淋巴细胞产生IL-17的影响及其机制。方法 复制哮喘小鼠模型，体外分离培养脾脏来源的T淋巴细胞，分为对照组和LPS组，其中LPS组又分为LPS1组和LPS2组，分别以1、2 ng/ml的LPS体外刺激T细胞72 h。对照组以生理盐水代替LPS。采用ELISA法检测细胞培养上清液中的IL-17水平，观察IL-17的水平浓度变化。结果 以低剂量LPS刺激哮喘小鼠T淋巴细胞后，其培养上清液中IL-17的水平较对照组明显降低，差异有统计学意义(P<0.01)。结论 低剂量LPS可以抑制哮喘T细胞IL-17的分泌，可能与机体的免疫耐受相关。
[关键词]脂多糖；哮喘小鼠；T细胞；白介素-17
[中图分类号] R-332 [文献标识码] A [文章编号] 1674-4721(2017)01(c)-0120-03
[Abstract]Objective To explore the influence of LPS with low dosage on the secretion of IL-17 and the potential mechanism.Methods The asthma mouse model was copied successfully and the T cells from spleen were isolated and cultured in vitro ......
您现在查看是摘要页，全文长 3340 字符。
[关键词]脂多糖；哮喘小鼠；T细胞；白介素-17
[中图分类号] R-332 [文献标识码] A [文章编号] 1674-4721(2017)01(c)-0120-03
[Abstract]Objective To explore the influence of LPS with low dosage on the secretion of IL-17 and the potential mechanism.Methods The asthma mouse model was copied successfully and the T cells from spleen were isolated and cultured in vitro ......
您现在查看是摘要页，全文长 3340 字符。