RUNX3和p53在结直肠锯齿状病变及结直肠癌组织中的表达及意义(1)
[摘要]目的 探讨RUNX3和p53在结直肠锯齿状病变及结直肠癌组织中的表达及意义。方法 选择2012年1月～2018年1月我院病理科确诊的262例结直肠锯齿状病变，其中增生性息肉(HP)87例，广基锯齿状息肉/腺瘤(SSA/P)96例，传统锯齿状腺瘤(TSA)79例;另选取60例正常肠黏膜标本以及60例结直肠癌(CRC)标本。通过免疫组化染色对各标本中RUNX3和p53的表达进行分析和评价。结果 RUNX3阳性表达率在正常肠黏膜、HP、SSA/P、TSA、CRC中逐渐降低，在SSA/P、TSA及CRC中明显降低;SSA/P、TSA、CRC与正常肠黏膜及HP的RUNX3阳性率比较，差异有统计学意义(P<0.05)。p53阳性表达率在正常肠黏膜、HP、SSA/P、TSA、CRC中逐渐升高，在SSA/P、TSA及CRC中明显升高;SSA/P、TSA、CRC与正常肠黏膜及HP的p53阳性率比较，差异有统计学意义(P<0.05)。结论 RUNX3和p53在锯齿状病变到CRC的发生过程中起作用，两者可能是促进锯齿状腺瘤恶变的危险因素，对锯齿状腺瘤的患者预测CRC的发生具有一定的临床价值。
[关键词]RUNX3;p53;结直肠锯齿状病变;免疫组化
[中图分类号] R734.2 [文献标识码] A [文章编号] 1674-4721(2019)7(b)-0040-03
[Abstract] Objective To investigate the expression and significance of RUNX3 and p53 in colorectal serrated lesions and colorectal cancer. Methods A total of 262 specimens of colorectal serrated lesions diagnosed in the pathology department of our hospital from January 2012 to January 2018 were selected， including 87 cases of hyperplastic polyps (HP)， 96 cases of sessile serrated adenoma/polyp (SSA/P)， and 79 cases of traditional serrated adenoma (TSA). Another 60 normal intestinal mucosa and 60 colorectal cancer (CRC) specimens were selected. The expression of RUNX3 and p53 in each specimen was analyzed and evaluated by immunohistochemical staining. Results The positive expression rate of RUNX3 gradually decreased in normal intestinal mucosa， HP， SSA/P， TSA， CRC， and significantly decreased in SSA/P， TSA and CRC. The positive rate of RUNX3 between SSA/P， TSA， CRC and normal intestinal mucosa， HP was statistically significant (P<0.05). The positive expression rate of p53 increased gradually in normal intestinal mucosa， HP， SSA/P， TSA， CRC， and increased significantly in SSA/P， TSA and CRC. The positive rate of p53 between SSA/P， TSA， CRC and normal intestinal mucosa， HP was statistically significant (P<0.05). Conclusion RUNX3 and p53 play the roles in the occurrence of serrated lesions to CRC. They may be the risk factors of the progress of serrated adenoma to adenocarcinoma. The detection of RUNX3 and p53 in serrated adenoma patients can help to predict the occurrence of CRC.
[Key words] RUNX3; p53; Colorectal serrated lesion; Immunohistochemistry
結直肠癌(colorectal cancer，CRC)是我国最常见的恶性肿瘤之一。国外有研究显示大部分的CRC是由“腺瘤-癌”的经典途径发展而来，约35%的CRC是由锯齿状途径发展而来[1]。锯齿状腺瘤(serrated adenoma，SA)的恶变率较高，发展迅速，因此引起人们的广泛关注。WHO(2010)消化系统肿瘤新分类把锯齿状病变分为4类[2]：增生性息肉(hyperplastic polyp，HP)、广基锯齿状息肉/腺瘤(sessile serrated adenoma/polyp，SSA/P)、传统锯齿状腺瘤(traditional serrated adenoma，TSA)、锯齿状腺癌(serrated adenocarcinoma，SAC)。这些病变经历了多个阶段，有多种因素及多个基因共同参与，引起基因学及多种细胞信号转导通路的异常，最终导致锯齿状病变发展为CRC[3-7]。, http://www.100md.com(赵巍 李琳 张肖洁 何英 蒋萍 龙培滨 柳平平 王丹丹)
[关键词]RUNX3;p53;结直肠锯齿状病变;免疫组化
[中图分类号] R734.2 [文献标识码] A [文章编号] 1674-4721(2019)7(b)-0040-03
[Abstract] Objective To investigate the expression and significance of RUNX3 and p53 in colorectal serrated lesions and colorectal cancer. Methods A total of 262 specimens of colorectal serrated lesions diagnosed in the pathology department of our hospital from January 2012 to January 2018 were selected， including 87 cases of hyperplastic polyps (HP)， 96 cases of sessile serrated adenoma/polyp (SSA/P)， and 79 cases of traditional serrated adenoma (TSA). Another 60 normal intestinal mucosa and 60 colorectal cancer (CRC) specimens were selected. The expression of RUNX3 and p53 in each specimen was analyzed and evaluated by immunohistochemical staining. Results The positive expression rate of RUNX3 gradually decreased in normal intestinal mucosa， HP， SSA/P， TSA， CRC， and significantly decreased in SSA/P， TSA and CRC. The positive rate of RUNX3 between SSA/P， TSA， CRC and normal intestinal mucosa， HP was statistically significant (P<0.05). The positive expression rate of p53 increased gradually in normal intestinal mucosa， HP， SSA/P， TSA， CRC， and increased significantly in SSA/P， TSA and CRC. The positive rate of p53 between SSA/P， TSA， CRC and normal intestinal mucosa， HP was statistically significant (P<0.05). Conclusion RUNX3 and p53 play the roles in the occurrence of serrated lesions to CRC. They may be the risk factors of the progress of serrated adenoma to adenocarcinoma. The detection of RUNX3 and p53 in serrated adenoma patients can help to predict the occurrence of CRC.
[Key words] RUNX3; p53; Colorectal serrated lesion; Immunohistochemistry
結直肠癌(colorectal cancer，CRC)是我国最常见的恶性肿瘤之一。国外有研究显示大部分的CRC是由“腺瘤-癌”的经典途径发展而来，约35%的CRC是由锯齿状途径发展而来[1]。锯齿状腺瘤(serrated adenoma，SA)的恶变率较高，发展迅速，因此引起人们的广泛关注。WHO(2010)消化系统肿瘤新分类把锯齿状病变分为4类[2]：增生性息肉(hyperplastic polyp，HP)、广基锯齿状息肉/腺瘤(sessile serrated adenoma/polyp，SSA/P)、传统锯齿状腺瘤(traditional serrated adenoma，TSA)、锯齿状腺癌(serrated adenocarcinoma，SAC)。这些病变经历了多个阶段，有多种因素及多个基因共同参与，引起基因学及多种细胞信号转导通路的异常，最终导致锯齿状病变发展为CRC[3-7]。, http://www.100md.com(赵巍 李琳 张肖洁 何英 蒋萍 龙培滨 柳平平 王丹丹)