三种支气管肺泡灌洗液结核杆菌检测方法在菌阴肺结核诊断中的应用研究
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【摘要】 目的 探讨涂片、培养、荧光定量PCR(FQ-PCR)检测支气管肺泡灌洗液(BALF)结核分枝杆菌对菌阴肺结核的诊断价值。方法 对73例菌阴肺结核患者的BALF行涂片、培养、FQ-PCR同步检测。结果 菌阴肺结核患者的BALF涂片、培养、FQ-PCR检查的阳性率分别为21.9%、34.2%和60.3%，FQ-PCR法敏感度高且假阳性低(占2.2%)。结论 BALF涂片和FQ-PCR检测对菌阴肺结核的快速诊断具有重要的临床应用价值，培养仍是目前诊断肺结核的金标准；三种方法结合使用可提高菌阴肺结核的诊断率。
【关键词】 支气管肺泡灌洗液； 荧光定量PCR； 菌阴肺结核
Study on three detection ways of bronchoalveolar lavage fluid TB bacilli in the application of smear-negative pulmonary tuberculosis diagnose ZHANG Yan.Yongzhou People's Hospital in Hunan Province,Yongzhou 425001,China
【Abstract】 Objective To explore the values of smearing,training and PCR in the detection of bronchoalveolar lavage fluid(BALF) lepae when diagnising smear-negative pulmonary tuberculosis.Methods Smearing, training and FQ-PCR on 76 cases of smear-negative pulmonary tuberculosis.Results The positive rate of balf smearing,training and the FQ-PCR were respectively 21.9%,34.2% and 60.3%.Way of FQ-PCR is sensitive and the false positive rate is low (2.2%).Conclusion Ways of smearing and FQ-PCR have the significant clinical value when diagnosing the smear-negative pulmonary tuberculosis in the first time and smearing is still the best one ......
【摘要】 目的 探讨涂片、培养、荧光定量PCR(FQ-PCR)检测支气管肺泡灌洗液(BALF)结核分枝杆菌对菌阴肺结核的诊断价值。方法 对73例菌阴肺结核患者的BALF行涂片、培养、FQ-PCR同步检测。结果 菌阴肺结核患者的BALF涂片、培养、FQ-PCR检查的阳性率分别为21.9%、34.2%和60.3%，FQ-PCR法敏感度高且假阳性低(占2.2%)。结论 BALF涂片和FQ-PCR检测对菌阴肺结核的快速诊断具有重要的临床应用价值，培养仍是目前诊断肺结核的金标准；三种方法结合使用可提高菌阴肺结核的诊断率。
【关键词】 支气管肺泡灌洗液； 荧光定量PCR； 菌阴肺结核
Study on three detection ways of bronchoalveolar lavage fluid TB bacilli in the application of smear-negative pulmonary tuberculosis diagnose ZHANG Yan.Yongzhou People's Hospital in Hunan Province,Yongzhou 425001,China
【Abstract】 Objective To explore the values of smearing,training and PCR in the detection of bronchoalveolar lavage fluid(BALF) lepae when diagnising smear-negative pulmonary tuberculosis.Methods Smearing, training and FQ-PCR on 76 cases of smear-negative pulmonary tuberculosis.Results The positive rate of balf smearing,training and the FQ-PCR were respectively 21.9%,34.2% and 60.3%.Way of FQ-PCR is sensitive and the false positive rate is low (2.2%).Conclusion Ways of smearing and FQ-PCR have the significant clinical value when diagnosing the smear-negative pulmonary tuberculosis in the first time and smearing is still the best one ......
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