脓毒症患者血清脂联素水平及其与炎症标志物的关系(1)
【摘要】 目的：通过检测血清脂联素、降钙素原(PCT)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平的变化，进而探讨脓毒症患者血清脂联素水平变化及其与炎症标志物的相关性。方法：选择2011年1月-2014年1月入住郑州市第三人民医院重症医学科脓毒症患者30例为脓毒症组，选择同期健康体检人员30例作为健康对照组。在确诊患者为脓毒症后空腹留取静脉血标本待测，并行当天急性生理学及慢性健康状况(APACHE)Ⅱ评分；健康对照组空腹留取静脉血标本待测。血标本送本院化验室检测入选者的血常规、血糖、血脂、C-反应蛋白(CRP)及PCT；采用酶联免疫吸附法(ELISA)测定脂联素、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的水平。结果：两组性别、年龄、BMI、血糖及血脂比较差异均无统计学意义(P>0.05)；脓毒症组血清脂联素水平低于对照组，差异有统计学意义(P<0.05)；脓毒症组白细胞、中性粒细胞百分比、CRP、PCT、TNF-α及IL-6水平均明显高于对照组，差异有统计学意义(P<0.01)；血清脂联素与APACHEⅡ评分呈明显负相关(r=-0.753，P<0.01)。脓毒症组血清脂联素浓度与CRP、PCT、TNF-α及IL-6均存在显著负相关(r分别为-0.356、-0.548、-0.281和-0.315，P<0.01)。结论：脓毒症患者血清脂联素水平降低，与多种炎症标志物具有相关性；脂联素参与了脓毒症患者的全身炎症反应，对判断脓毒症患者的病情严重程度及预后有一定的参考价值。
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【关键词】 脓毒症； 脂联素； 炎症标志物
【Abstract】 Objective：To detect the changes of serum adiponectin，procalcitonin(PCT)，tumor necrosis factor alpha(TNF-alpha)，interleukin-6(IL-6) level，and explore the changes of serum adiponectin level in patients with sepsis and its relationship with inflammatory markers.Method：30 cases were sepsis patients group which was selected from severe medicine department of the third people’s Hospital of Zheng Zhou city from January 2011 to January 2014，and 30 healthy persons were enrolled as healthy control group.Tested fast peripheral blood specimens In patients diagnosed with sepsis before breakfast and scored acute physiology and chronic health conditions examination(APACHE) II at the very day； so do the healthy control group as well.Bloodspecimens were sent to the hospital laboratory for the blood glucose，blood lipid， blood routine test of C-reactive protein(CRP) and the PCT testing；the adiponectin， tumor necrosis factor alpha(TNF-alpha)，interleukin-6 (IL-6)level were tested by enzyme linked immunosorbent assay(ELISA).Result：There were no significant in two groups of gender，age，BMI，blood glucose and blood lipid differences(P>0.05).The serum adiponectin level of sepsis group was lower than that of the control group(P<0.05)，the difference was statistically significant as well；the WBC，neutrophil percentage，CRP，PCT，TNF-alpha and IL-6 in sepsis group was significantly higher than that in the control group(P<0.01)，the difference was statistically significant；and there was a negative correlation between serum adiponectin and APACHE score(r=-0.753，P<0.01).There was a significant negative correlation between the serum adiponectin concentration and CRP，PCT，TNF-alpha and IL-6(r=-0.356，-0.548，-0.281 and -0.315，P<0.01).Conclusion：The serum adiponectin level of patients with sepsis decreased ，which has a correlation with the inflammatory markers；adiponectin might play a role in the systemic inflammatory response in septic patients，that may have certain reference value for judging severity and prognosis of the sepsis patients., 百拇医药(李国吾 武玉荣)
, http://www.100md.com
【关键词】 脓毒症； 脂联素； 炎症标志物
【Abstract】 Objective：To detect the changes of serum adiponectin，procalcitonin(PCT)，tumor necrosis factor alpha(TNF-alpha)，interleukin-6(IL-6) level，and explore the changes of serum adiponectin level in patients with sepsis and its relationship with inflammatory markers.Method：30 cases were sepsis patients group which was selected from severe medicine department of the third people’s Hospital of Zheng Zhou city from January 2011 to January 2014，and 30 healthy persons were enrolled as healthy control group.Tested fast peripheral blood specimens In patients diagnosed with sepsis before breakfast and scored acute physiology and chronic health conditions examination(APACHE) II at the very day； so do the healthy control group as well.Bloodspecimens were sent to the hospital laboratory for the blood glucose，blood lipid， blood routine test of C-reactive protein(CRP) and the PCT testing；the adiponectin， tumor necrosis factor alpha(TNF-alpha)，interleukin-6 (IL-6)level were tested by enzyme linked immunosorbent assay(ELISA).Result：There were no significant in two groups of gender，age，BMI，blood glucose and blood lipid differences(P>0.05).The serum adiponectin level of sepsis group was lower than that of the control group(P<0.05)，the difference was statistically significant as well；the WBC，neutrophil percentage，CRP，PCT，TNF-alpha and IL-6 in sepsis group was significantly higher than that in the control group(P<0.01)，the difference was statistically significant；and there was a negative correlation between serum adiponectin and APACHE score(r=-0.753，P<0.01).There was a significant negative correlation between the serum adiponectin concentration and CRP，PCT，TNF-alpha and IL-6(r=-0.356，-0.548，-0.281 and -0.315，P<0.01).Conclusion：The serum adiponectin level of patients with sepsis decreased ，which has a correlation with the inflammatory markers；adiponectin might play a role in the systemic inflammatory response in septic patients，that may have certain reference value for judging severity and prognosis of the sepsis patients., 百拇医药(李国吾 武玉荣)