综合康复对大鼠受压坐骨神经微血管生成的影响(1)
【摘要】 目的：观察综合康复对大鼠受压坐骨神经微血管生成的影响。方法：参照经典Mackinnon大鼠坐骨神经卡压模型制作动物模型，40只大鼠完全随机分为A、B、C、D 4组，每组10只，2周后，A组(对照组)：仅去除卡压；B组(康复训练组)：去除卡压后行康复训练；C组(电针组)：去除卡压后行电针治疗；D组(康复训练+电针组)：去除卡压后行康复训练加电针治疗。术后第3周各组治疗结束。观察各组大鼠大体情况、坐骨神经功能指数(SFI)、卡压神经中段标本CD34、VEGF表达。结果：B、C、D组SFI恢复程度均大于A组(P<0.05)；D组SFI恢复程度明显大于B组与C组(P<0.05)。B、C、D组CD34、VEGF表达明显高于A组(P<0.05)；D组CD34、VEGF表达明显高于B组与C组(P<0.05)。结论：综合康复可以有效促进大鼠受压坐骨神经微血管生成，进而促进神经再生与功能恢复。
【关键词】 综合康复； 神经微血管生成； 神经再生； 坐骨神经
Effect of Comprehensive Rehabilitation on Neural Micro-angiogenesis in Rat with Sciatic Nerve Compression/ZHANG Shan-shan，DU Zhen-sheng，CHEN Feng，et al.//Medical Innovation of China，2015，12(18)：015-017
, http://www.100md.com
【Abstract】 Objective：To observe the the effect of comprehensive rehabilitation on neural micro-angiogenesis in rat with sciatic nerve compression.Method：According to the rat model of sciatic nerve compression designed by Mackinnon，forty male SD rats were chosen and randomly divided into 4 groups(n=10)：group A，group B，group C，and group D.After 2 weeks，compression were removed in all 4 groups，and no treatment was given in group A，rehabilitation training in group B，electroacupuncture in group C，electroacupuncture combined with rehabilitation training in group D.At 3 weeks after treatment，the sciatic nerve function index (SFI)，and the expression of CD34，VEGF in the compression sciatic nerve of rats were measured.Result：The SFI recovery rate of groups B，C，and D were significantly higher than that of group A(P<0.05)，and that of group D was significantly higher than that of groups B and C(P<0.05).The expression of CD34 and VEGF in the compression sciatic nerve of groups B，C，and D were significantly higher than that of group A(P<0.05)，and that of group D was significantly higher than that of groups B and C(P<0.05).Conclusion：Comprehensive rehabilitation can improve neural micro-angiogenesis in rat with sciatic nerve compression，thereby it can promote the nerve regeneration and functional recovery.
, 百拇医药
【Key words】 Comprehensive rehabilitation； Neural micro-angiogenesis； Nerve regeneration； Ciatic nerve
First-author’s address：Shenzhen No.2 People’s Hospital，Shenzhen 518000，China
doi：10.3969/j.issn.1674-4985.2015.18.005
目前，随着人民生活质量意识提高，周围神经损伤后恢复日益受到重视。虽然显微外科手术可以解除周围神经卡压，但如何保护卡压神经，最大限度地减轻继发性损害，重建神经传导，当前还无法很好解决。近年来，运用物理疗法治疗周围神经损伤已有报道[1-4]，但治疗机制仍不清楚，基于此，本课题采用经典Mackinnon[5]大鼠坐骨神经卡压模型，采用康复训练联合针刺疗法，通过对卡压神经CD34、VEGF表达检测以及坐骨神经功能指数检测，分析、揭示康复训练联合针刺疗法对卡压周围神经微血管生成及功能修复的作用机制，为临床从神经微循环角度治疗神经损伤提供实验依据。, 百拇医药(张珊珊 杜震生 陈峰 苏宁)
【关键词】 综合康复； 神经微血管生成； 神经再生； 坐骨神经
Effect of Comprehensive Rehabilitation on Neural Micro-angiogenesis in Rat with Sciatic Nerve Compression/ZHANG Shan-shan，DU Zhen-sheng，CHEN Feng，et al.//Medical Innovation of China，2015，12(18)：015-017
, http://www.100md.com
【Abstract】 Objective：To observe the the effect of comprehensive rehabilitation on neural micro-angiogenesis in rat with sciatic nerve compression.Method：According to the rat model of sciatic nerve compression designed by Mackinnon，forty male SD rats were chosen and randomly divided into 4 groups(n=10)：group A，group B，group C，and group D.After 2 weeks，compression were removed in all 4 groups，and no treatment was given in group A，rehabilitation training in group B，electroacupuncture in group C，electroacupuncture combined with rehabilitation training in group D.At 3 weeks after treatment，the sciatic nerve function index (SFI)，and the expression of CD34，VEGF in the compression sciatic nerve of rats were measured.Result：The SFI recovery rate of groups B，C，and D were significantly higher than that of group A(P<0.05)，and that of group D was significantly higher than that of groups B and C(P<0.05).The expression of CD34 and VEGF in the compression sciatic nerve of groups B，C，and D were significantly higher than that of group A(P<0.05)，and that of group D was significantly higher than that of groups B and C(P<0.05).Conclusion：Comprehensive rehabilitation can improve neural micro-angiogenesis in rat with sciatic nerve compression，thereby it can promote the nerve regeneration and functional recovery.
, 百拇医药
【Key words】 Comprehensive rehabilitation； Neural micro-angiogenesis； Nerve regeneration； Ciatic nerve
First-author’s address：Shenzhen No.2 People’s Hospital，Shenzhen 518000，China
doi：10.3969/j.issn.1674-4985.2015.18.005
目前，随着人民生活质量意识提高，周围神经损伤后恢复日益受到重视。虽然显微外科手术可以解除周围神经卡压，但如何保护卡压神经，最大限度地减轻继发性损害，重建神经传导，当前还无法很好解决。近年来，运用物理疗法治疗周围神经损伤已有报道[1-4]，但治疗机制仍不清楚，基于此，本课题采用经典Mackinnon[5]大鼠坐骨神经卡压模型，采用康复训练联合针刺疗法，通过对卡压神经CD34、VEGF表达检测以及坐骨神经功能指数检测，分析、揭示康复训练联合针刺疗法对卡压周围神经微血管生成及功能修复的作用机制，为临床从神经微循环角度治疗神经损伤提供实验依据。, 百拇医药(张珊珊 杜震生 陈峰 苏宁)