FISH检测痰脱落细胞3、8、9、17染色体异常情况，研究肺癌诊断新手段(1)
【摘要】 目的：应用荧光原位杂交(fluorescence in situ hybridization，FISH)技术对痰脱落细胞染色体异常情况进行分析，探讨FISH辅助诊断肺癌的可行性和有效性。方法：选择2014年6月-2015年6月本院收治的疑似肺癌患者30例作为研究对象，采用3、8、9、17号染色体着丝粒探针对其痰脱落细胞进行FISH检测并进行痰脱落细胞学检查。结果：肺癌确诊患者痰脱落细胞中3、8、9、17号染色体畸变阳性率分别为41.67%、45.83%、58.33%、41.67%；FISH检测和痰脱落细胞学检查的敏感度分别为83.33%和20.83%，特异性分别为66.67%和100%，诊断符合率分别为80.00%和36.67%，FISH检测敏感度和诊断符合率均高于痰脱落细胞学检查，比较差异均有统计学意义(P<0.05)。结论：肺癌的发生发展与染色体畸变有关，FISH检查可明显提高肺癌诊断准确率，可作为肺癌诊断新手段。
【关键词】 肺癌； 荧光原位杂交； 痰脱落细胞
, http://www.100md.com
【Abstract】 Objective：To analyze the chromosome abnormalities of exfoliated cells in sputum samples by fluorescence in situ hybridization and to explore the possibility and validity of FISH examination for the diagnosis of lung cancer.Method：30 patients who were suspected with lung cancer in our hospital from June 2014 to June 2015 were selected as the research objects.They were given FISH examination by using centromeric probes of chromosome 3，8，9，17 to examine chromosome abnormalities of sputum exfoliated cells，sputum cytology detection was also given to them.Result：The aberration rate of chromosomes 3，8，9 and 17 in lung cancer patients’ sputum exfoliated cells were 41.67%，45.83%，58.33% and 41.67% respectively.The sensitivity of FISH and sputum cytology detection were 83.33% and 20.83% respectively，the specificity of them were 66.67% and 100% respectively and the diagnostic accordance rate of them were 80.00% and 36.67% respectively.The sensitivity and the diagnostic accordance rate of the FISH examination were higher than those of the sputum cytology detection，the differences were statistically significant(P<0.05).Conclusion：Chromosome aberrations plays an important role in the development of lung cancer.FISH technique can significantly improve the accuracy rate of diagnosis for lung cancer.It can be used as a new mean for the diagnosis of lung cancer.
, 百拇医药
【Key words】 Lung cancer； Fluorescence in situ hybridization； Sputum exfoliated cell
First-author’s address：The Tumor Hospital of Jiangxi Province，Nanchang 330029，China
doi：10.3969/j.issn.1674-4985.2015.32.001
肺癌是当今世界上最常见的威胁人类生命健康的恶性肿瘤[1]。近年来由于女性吸烟人群数量的逐渐增加，肺癌总发病率也随之显著增加[2]。据相关统计学结果表明，肺癌的发病率和死亡率均占我国大多数城市恶性肿瘤的首位。影像学检查如胸部X射线、CT检查、肺穿刺活检，支气管镜检查和痰脱落细胞学检查均是临床诊断肺癌的有效手段，MiRNA作为血清标志物也为肺癌早期诊断提供了新的研究途径[3-4]。与其他方法相比，痰脱落细胞学检查具有取材简单、安全、患者无创伤等优点，且可以判断肿瘤的组织学类型，对肺癌的治疗有重要意义。荧光原位杂交(fluorescence in situ hybridization，FISH)技术是一门新兴的分子细胞遗传学技术，可用于检测染色体数目和结构的异常变化，为恶性肿瘤的研究提供了新的技术支持，目前被广泛用于诊断尿路上皮癌、血液系统肿瘤以及宫颈癌等恶性肿瘤[5]。本研究利用FISH技术检测30例疑似肺癌患者痰脱落细胞3、8、9、17号染色体异常情况，探讨FISH作为肺癌诊断新手段的应用价值，现报道如下。, http://www.100md.com(李林)
【关键词】 肺癌； 荧光原位杂交； 痰脱落细胞
, http://www.100md.com
【Abstract】 Objective：To analyze the chromosome abnormalities of exfoliated cells in sputum samples by fluorescence in situ hybridization and to explore the possibility and validity of FISH examination for the diagnosis of lung cancer.Method：30 patients who were suspected with lung cancer in our hospital from June 2014 to June 2015 were selected as the research objects.They were given FISH examination by using centromeric probes of chromosome 3，8，9，17 to examine chromosome abnormalities of sputum exfoliated cells，sputum cytology detection was also given to them.Result：The aberration rate of chromosomes 3，8，9 and 17 in lung cancer patients’ sputum exfoliated cells were 41.67%，45.83%，58.33% and 41.67% respectively.The sensitivity of FISH and sputum cytology detection were 83.33% and 20.83% respectively，the specificity of them were 66.67% and 100% respectively and the diagnostic accordance rate of them were 80.00% and 36.67% respectively.The sensitivity and the diagnostic accordance rate of the FISH examination were higher than those of the sputum cytology detection，the differences were statistically significant(P<0.05).Conclusion：Chromosome aberrations plays an important role in the development of lung cancer.FISH technique can significantly improve the accuracy rate of diagnosis for lung cancer.It can be used as a new mean for the diagnosis of lung cancer.
, 百拇医药
【Key words】 Lung cancer； Fluorescence in situ hybridization； Sputum exfoliated cell
First-author’s address：The Tumor Hospital of Jiangxi Province，Nanchang 330029，China
doi：10.3969/j.issn.1674-4985.2015.32.001
肺癌是当今世界上最常见的威胁人类生命健康的恶性肿瘤[1]。近年来由于女性吸烟人群数量的逐渐增加，肺癌总发病率也随之显著增加[2]。据相关统计学结果表明，肺癌的发病率和死亡率均占我国大多数城市恶性肿瘤的首位。影像学检查如胸部X射线、CT检查、肺穿刺活检，支气管镜检查和痰脱落细胞学检查均是临床诊断肺癌的有效手段，MiRNA作为血清标志物也为肺癌早期诊断提供了新的研究途径[3-4]。与其他方法相比，痰脱落细胞学检查具有取材简单、安全、患者无创伤等优点，且可以判断肿瘤的组织学类型，对肺癌的治疗有重要意义。荧光原位杂交(fluorescence in situ hybridization，FISH)技术是一门新兴的分子细胞遗传学技术，可用于检测染色体数目和结构的异常变化，为恶性肿瘤的研究提供了新的技术支持，目前被广泛用于诊断尿路上皮癌、血液系统肿瘤以及宫颈癌等恶性肿瘤[5]。本研究利用FISH技术检测30例疑似肺癌患者痰脱落细胞3、8、9、17号染色体异常情况，探讨FISH作为肺癌诊断新手段的应用价值，现报道如下。, http://www.100md.com(李林)