胸腺瘤组织中c—myc和PTEN基因的表达(1)
【摘要】 目的：探讨c-myc、PTEN基因在胸腺瘤中的表达及其与胸腺瘤临床病理特征的关系。方法：采用免疫组织化学SP法检测45例胸腺瘤(胸腺瘤组)和16例非肿瘤(对照组)胸腺组织中c-myc、PTEN基因的表达。结果：胸腺瘤组c-myc基因阳性表达率高于对照组，PTEN基因阳性表达率低于对照组，比较差异均有统计学意义(P<0.01)；恶性组c-myc基因阳性表达率高于良性组，PTEN基因阳性表达率低于良性组，比较差异均有统计学意义(P<0.05)；侵袭性组c-myc基因阳性表达率高于非侵袭性组，PTEN基因阳性表达率低于非侵袭性组，比较差异均有统计学意义(P<0.01)；PTEN基因表达与c-myc表达呈负相关(P<0.01)。结论：c-myc、PTEN共同参与胸腺瘤的发生、发展，与胸腺瘤的恶性程度及侵袭性呈正相关，二者存在协同作用。
【关键词】 胸腺瘤； c-myc基因； PTEN基因
【Abstract】 Objective：To evaluate the expression of c-myc and PTEN in thymomas and their correlation with clinical pathological characteristics of thymomas.Method：Immunohistochemical SP assay was used to detect the expression of c-myc and PTEN in thymus issues of 45 thymoma patients(the thymoma group)and 16 non-neoplastic patients(the control group).Result：The positive expression rate of c-myc in the thymoma group was higher than that in the control group，the positive expression rate of PTEN in the thymoma group was lower than that in the control group，the differences were statistically significant(P<0.01).The positive expression rate of c-myc in the malignancy group was higher than that in the benign group，the positive expression rate of PTEN in the malignancy group was lower than that in the benign group，the differences were statistically significant(P<0.05).The positive expression rate of c-myc in the invasiveness group was higher than that in the non-invasive group，the positive expression rate of PTEN in the invasiveness group was lower than that in the non-invasive group，the differences were statistically significant(P<0.01).There was a negative correlation between the expression of PTEN gene and the expression of c-myc gene(P<0.01).Conclusion：C-myc and PTEN may play an important role in the occurrence and development of thymoma.The expression of c-myc and PTEN correlates with the malignance and the invasiveness of thymoma.They have a synergistic effect.
, 百拇医药
【Key words】 Thymoma； C-myc gene； PTEN gene
First-author’s address：The First Affiliated Hospital of Nanyang Medical College，Nanyang 473058，China
doi：10.3969/j.issn.1674-4985.2015.32.013
胸腺瘤是常见的纵隔肿瘤，一般位于前上纵隔，被认为是胸腺上皮肿瘤。胸腺瘤具有局部浸润、远处转移等特性，但其属于低度恶性或者潜在恶性肿瘤[1]。由于其发病率低且经常伴随自身免疫疾病以及其他系统病变，发病机制复杂，故在其病因、病理分类、临床分期、治疗方案等诸多方面一直存在争议。本研究应用免疫组织化学SP法探讨癌基因c-myc及抑癌基因PTEN在胸腺瘤组织中的表达，结合Masaoka分期以及WHO分型，研究其在胸腺瘤发生发展过程中的作用、其与胸腺瘤临床病理学特征的关系以及二者在胸腺瘤组织中表达的相关性，期望为临床诊断和治疗胸腺瘤提供理论依据，现具体报道如下。
1 资料与方法
1.1 一般资料 选择2001年1月-2012年1月本院45例患者的胸腺瘤切除标本存档蜡块作为研究材料，所选患者中男26例，女l9例，年龄20～72岁，平均43.4岁。非侵袭性胸腺瘤(I期)17例(非侵袭性组)；侵袭性胸腺瘤28例(侵袭性组)，其中Ⅱ期11例，Ⅲ期14例，Ⅳ期3例。良性或低度恶性胸腺瘤14例(良性组)，其中A型5例，AB型9例；恶性胸腺瘤31例(恶性组)，其中B1型8例，B2型11例，B3型12例。取同期16例非肿瘤胸腺组织作为对照组。, 百拇医药(石锋)
【关键词】 胸腺瘤； c-myc基因； PTEN基因
【Abstract】 Objective：To evaluate the expression of c-myc and PTEN in thymomas and their correlation with clinical pathological characteristics of thymomas.Method：Immunohistochemical SP assay was used to detect the expression of c-myc and PTEN in thymus issues of 45 thymoma patients(the thymoma group)and 16 non-neoplastic patients(the control group).Result：The positive expression rate of c-myc in the thymoma group was higher than that in the control group，the positive expression rate of PTEN in the thymoma group was lower than that in the control group，the differences were statistically significant(P<0.01).The positive expression rate of c-myc in the malignancy group was higher than that in the benign group，the positive expression rate of PTEN in the malignancy group was lower than that in the benign group，the differences were statistically significant(P<0.05).The positive expression rate of c-myc in the invasiveness group was higher than that in the non-invasive group，the positive expression rate of PTEN in the invasiveness group was lower than that in the non-invasive group，the differences were statistically significant(P<0.01).There was a negative correlation between the expression of PTEN gene and the expression of c-myc gene(P<0.01).Conclusion：C-myc and PTEN may play an important role in the occurrence and development of thymoma.The expression of c-myc and PTEN correlates with the malignance and the invasiveness of thymoma.They have a synergistic effect.
, 百拇医药
【Key words】 Thymoma； C-myc gene； PTEN gene
First-author’s address：The First Affiliated Hospital of Nanyang Medical College，Nanyang 473058，China
doi：10.3969/j.issn.1674-4985.2015.32.013
胸腺瘤是常见的纵隔肿瘤，一般位于前上纵隔，被认为是胸腺上皮肿瘤。胸腺瘤具有局部浸润、远处转移等特性，但其属于低度恶性或者潜在恶性肿瘤[1]。由于其发病率低且经常伴随自身免疫疾病以及其他系统病变，发病机制复杂，故在其病因、病理分类、临床分期、治疗方案等诸多方面一直存在争议。本研究应用免疫组织化学SP法探讨癌基因c-myc及抑癌基因PTEN在胸腺瘤组织中的表达，结合Masaoka分期以及WHO分型，研究其在胸腺瘤发生发展过程中的作用、其与胸腺瘤临床病理学特征的关系以及二者在胸腺瘤组织中表达的相关性，期望为临床诊断和治疗胸腺瘤提供理论依据，现具体报道如下。
1 资料与方法
1.1 一般资料 选择2001年1月-2012年1月本院45例患者的胸腺瘤切除标本存档蜡块作为研究材料，所选患者中男26例，女l9例，年龄20～72岁，平均43.4岁。非侵袭性胸腺瘤(I期)17例(非侵袭性组)；侵袭性胸腺瘤28例(侵袭性组)，其中Ⅱ期11例，Ⅲ期14例，Ⅳ期3例。良性或低度恶性胸腺瘤14例(良性组)，其中A型5例，AB型9例；恶性胸腺瘤31例(恶性组)，其中B1型8例，B2型11例，B3型12例。取同期16例非肿瘤胸腺组织作为对照组。, 百拇医药(石锋)