CD4+CD25+Foxp3+Tregs在自身免疫性甲状腺疾病中的作用研究(1)
【摘要】 目的：探討CD4+CD25+Foxp3+Tregs在自身免疫性甲状腺疾病(AITD)中的作用。方法：收集2014年2月-2016年11月在本院进行检查的AITD患者80例为治疗组，其中Graves(GD)患者40例，为GD组；桥本甲状腺炎(HT)患者40例，为HT组；选取健康体检人员20例为对照组。采用流式细胞术检测外周血CD4+T、CD4+CD25+Tregs细胞数量和比例，采用RT-PCR检测外周血Foxp3 mRNA表达水平，采用化学发光法对血清中甲状腺素(TSH)、总三碘甲状腺原氨酸(TT3)、总甲状腺素(TT4)、甲状腺球蛋白抗体(TgAb)和甲状腺过氧化酶抗体(TPOAb)的水平进行测定。结果：三组TSH、TT3、TT4、TgAb、TPOAb比较，差异均有统计学意义(P<0.05)；三组外周血CD4+T细胞数量比较，差异无统计学意义(P>0.05)；GD组外周血CD4+CD25+Tregs占CD4+T细胞的比率与对照组比较，差异无统计学意义(P>0.05)；HT组外周血CD4+CD25+Tregs占CD4+T细胞的比率明显低于对照组，差异有统计学意义(P<0.05)；GD组和HT组外周血Foxp3 mRNA表达量均明显低于对照组，差异均有统计学意义(P<0.05)；自身免疫性甲状腺疾病的CD4+CD25+Foxp3+Tregs与TSH、TT3、TT4、TgAb和TPOAb有明显正相关性(P<0.05)。结论：CD4+CD25+Foxp3+Tregs可能在AITD的发生发展进程中起着一定的作用，深入研究CD4+CD25+Foxp3+Tregs在AITD中的作用将有助于建立新的预防和治疗策略。
【关键词】 自身免疫性甲状腺疾病； Graves； 桥本甲状腺炎； CD4+CD25+Foxp3+
【Abstract】 Objective：To study the role of CD4+CD25+Foxp3+Tregs in autoimmune thyroid diseases(AITD).Method：80 cases of AITD were collected from February 2014 to November 2016 in our hospital.Included 40 patients with Graves(GD)，as GD group，40 patients with Hashimoto's thyroiditis(HT)，as HT group，20 cases healthy people were selected as the control group.The peripheral blood flow cytometric detection of CD4+T and the number and proportion of CD4+CD25+Tregs cells were detected by flow cytometry，the peripheral blood Foxp3 expression level was detected by RT-PCR，the levels of total thyroxine(TSH)，total three triiodothyronine(TT3)，total thyroxine(TT4)，thyroglobulin antibody(TgAb) and thyroid peroxidase(TPOAb) were measured by chemiluminescence method.Result：TSH，TT3，TT4，TgAb，TPOAb levels of three groups had significant differences(P<0.05).The peripheral blood of CD4+T of three groups had no significant difference(P>0.05).Peripheral blood CD4+CD25+Tregs cells and the ratio of CD4+T of GD group compared with the control group，there were no significant differences(P>0.05).Peripheral blood CD4+CD25+Tregs cells and the ratio of CD4+T of HT group were significantly lower than those of the control group(P<0.05).Foxp3 mRNA expression of GD group and HT group were significantly lower than that the control group(P<0.05).CD4+CD25+Foxp3+Tregs of AITD was positively correlated with TSH，TT3，TT4，TgAb and TPOAb (P<0.05).Conclusion：CD4+CD25+Foxp3+Tregs may play an important role in the occurrence and development of AITD.Further research on the role of CD4+CD25+Foxp3+Tregs in AITD will help to establish a new prevention and treatment strategy.
【Key words】 Autoimmune thyroid disease； Graves； Hashimoto’s thyroiditis； CD4+CD25+Foxp3+Tregs, http://www.100md.com(黄广英 禤文婷 何灵杰)
【关键词】 自身免疫性甲状腺疾病； Graves； 桥本甲状腺炎； CD4+CD25+Foxp3+
【Abstract】 Objective：To study the role of CD4+CD25+Foxp3+Tregs in autoimmune thyroid diseases(AITD).Method：80 cases of AITD were collected from February 2014 to November 2016 in our hospital.Included 40 patients with Graves(GD)，as GD group，40 patients with Hashimoto's thyroiditis(HT)，as HT group，20 cases healthy people were selected as the control group.The peripheral blood flow cytometric detection of CD4+T and the number and proportion of CD4+CD25+Tregs cells were detected by flow cytometry，the peripheral blood Foxp3 expression level was detected by RT-PCR，the levels of total thyroxine(TSH)，total three triiodothyronine(TT3)，total thyroxine(TT4)，thyroglobulin antibody(TgAb) and thyroid peroxidase(TPOAb) were measured by chemiluminescence method.Result：TSH，TT3，TT4，TgAb，TPOAb levels of three groups had significant differences(P<0.05).The peripheral blood of CD4+T of three groups had no significant difference(P>0.05).Peripheral blood CD4+CD25+Tregs cells and the ratio of CD4+T of GD group compared with the control group，there were no significant differences(P>0.05).Peripheral blood CD4+CD25+Tregs cells and the ratio of CD4+T of HT group were significantly lower than those of the control group(P<0.05).Foxp3 mRNA expression of GD group and HT group were significantly lower than that the control group(P<0.05).CD4+CD25+Foxp3+Tregs of AITD was positively correlated with TSH，TT3，TT4，TgAb and TPOAb (P<0.05).Conclusion：CD4+CD25+Foxp3+Tregs may play an important role in the occurrence and development of AITD.Further research on the role of CD4+CD25+Foxp3+Tregs in AITD will help to establish a new prevention and treatment strategy.
【Key words】 Autoimmune thyroid disease； Graves； Hashimoto’s thyroiditis； CD4+CD25+Foxp3+Tregs, http://www.100md.com(黄广英 禤文婷 何灵杰)