野茼蒿对UC大鼠血清黏附分子表达的影响(1)
【摘要】 目的：通过研究野茼蒿对溃疡性结肠炎大鼠血清相关黏附分子表达的影响，探讨其对溃疡性结肠炎的疗效。方法：选择2，4，6-三硝基苯磺酸(TNBS)与乙醇混合液诱导的溃疡性结肠炎大鼠为研究对象，把UC模型大鼠分为：空白对照组、模型组，野茼蒿低、中、高剂量组，阳性对照组，每组各12只。每天给予各组不同药物处理。于给药21 d后对大鼠结肠组织进行CMDI和HS评分，并用ELISA法检测血清黏附分子CD62P、PSGL-1、CD44、CD54的含量，比较各组间指标差异。结果：与空白组相比，模型组CMDI评分和HS评分以及血清中黏附分子CD62P、PSGL-1、CD44、CD54明显升高(P<0.01或P<0.05)；与模型组比较，低、中、高剂量组大鼠上述两个评分以及黏附分子含量一定程度上降低(P<0.01或P<0.05)；与模型组相比，SASP组大鼠血清述两个评分以及黏附分子的含量明显降低(P<0.01或P<0.05)。结论：野茼蒿冻干粉对UC大鼠有一定的治疗作用，其机制可能与影响CD62P、PSGL-1、CD44、CD54的表达有关。
【关键词】 溃疡性结肠炎； 野茼蒿； 血清黏附分子
【Abstract】 Objective：To investigate the effect of Wild Chrysanthemum on the expression of serum-related adhesion molecules in rats with ulcerative colitis and to explore its effect on ulcerative colitis.Method：The rats of ulcerative colitis induced by 2，4，6-trinitrobenzene sulfonic acid(TNBS) and ethanol were selected as the study subjects.The UC rats were divided into six groups：blank group，model group，wild chrysanthemum Low，medium and high dose group，positive control group，12 cases in each group.Each group was given a different medication every day.At 21 days after administration，CMDI score and HS score were performed on rat colon tissue.The levels of CD62P，PSGL-1，CD44 and CD54 in serum were measured by ELISA.The differences of six groups were compared and analyzed statistically.Result：Compared with the blank group，the CMDI scores and HS scores and the levels of CD62P，PSGL-1，CD44 and CD54 in the serum of the model group were significantly increased(P<0.01 or P<0.05)；compared with the model group，the two scores and the levels of adhesion molecules in serum of the low，medium and high dose rats groups were significantly lower(P<0.01 or P<0.05)；compared with the model group，the above two scores and the content of adhesion molecules in serum of SASP group were significantly decreased(P<0.01 or P<0.05).Conclusion：The freeze-dried powder of Wild Chrysanthemum has a certain therapeutic effect on the clinical symptoms of UC rats.The mechanism may be related to the expression of CD62P，PSGL-1，CD44 and CD54.
【Key words】 Ulcerative colitis； Wild Chrysanthemum； Serum adhesio lecules
First-author’s address：Qiqihar Medical University，Qiqihar 161006，China
doi：10.3969/j.issn.1674-4985.2017.25.005
潰疡性结肠炎(Ulcerative Colitis，UC)是一种结肠和直肠慢性非特异性炎症性疾病，病因尚不明确，病变范围广泛。目前大多认为其发病机制与免疫因素、遗传因素、心理因素、饮食因素等有关，其中免疫异常被认为是最重要的发病因素。溃疡性结肠炎治愈难度大、病程长，经常反复发作，UC的发病率在我国呈升高的趋势。目前UC临床治疗以激素、水杨酸制剂和免疫抑制剂为主，但疗效欠佳，患者易产生耐药性、过敏等不良反应。中药在治疗UC上具有一定优势，避免了传统西药的弊端，在UC的治疗上显示出了广阔前景，临床上以中药灌肠治疗效果较好[1-2]。野茼蒿(Wild chrysanthemum)是菊科茼蒿属一年或二年生草本植物，在我国境内主要分布于广东、海南、四川、云南等地，是一种常用的药食两用植物，含有多种类型化合物，可治疗慢性肠胃炎[3-5]。黏附分子CD62P、CD44、CD54参与炎症反应中的重要环节，在UC的发病过程中起到了重要作用。本实验用2，4，6-三硝基苯磺酸(TNBS)与乙醇混合液诱导UC大鼠模型，将野茼蒿应用于UC大鼠，通过检测其血清中相关黏附分子的变化水平，以期探讨野茼蒿对UC的治疗机制，为其药用价值提供临床依据，现报道如下。, 百拇医药(彭凡珂 冯陈慧 寇力丹)
【关键词】 溃疡性结肠炎； 野茼蒿； 血清黏附分子
【Abstract】 Objective：To investigate the effect of Wild Chrysanthemum on the expression of serum-related adhesion molecules in rats with ulcerative colitis and to explore its effect on ulcerative colitis.Method：The rats of ulcerative colitis induced by 2，4，6-trinitrobenzene sulfonic acid(TNBS) and ethanol were selected as the study subjects.The UC rats were divided into six groups：blank group，model group，wild chrysanthemum Low，medium and high dose group，positive control group，12 cases in each group.Each group was given a different medication every day.At 21 days after administration，CMDI score and HS score were performed on rat colon tissue.The levels of CD62P，PSGL-1，CD44 and CD54 in serum were measured by ELISA.The differences of six groups were compared and analyzed statistically.Result：Compared with the blank group，the CMDI scores and HS scores and the levels of CD62P，PSGL-1，CD44 and CD54 in the serum of the model group were significantly increased(P<0.01 or P<0.05)；compared with the model group，the two scores and the levels of adhesion molecules in serum of the low，medium and high dose rats groups were significantly lower(P<0.01 or P<0.05)；compared with the model group，the above two scores and the content of adhesion molecules in serum of SASP group were significantly decreased(P<0.01 or P<0.05).Conclusion：The freeze-dried powder of Wild Chrysanthemum has a certain therapeutic effect on the clinical symptoms of UC rats.The mechanism may be related to the expression of CD62P，PSGL-1，CD44 and CD54.
【Key words】 Ulcerative colitis； Wild Chrysanthemum； Serum adhesio lecules
First-author’s address：Qiqihar Medical University，Qiqihar 161006，China
doi：10.3969/j.issn.1674-4985.2017.25.005
潰疡性结肠炎(Ulcerative Colitis，UC)是一种结肠和直肠慢性非特异性炎症性疾病，病因尚不明确，病变范围广泛。目前大多认为其发病机制与免疫因素、遗传因素、心理因素、饮食因素等有关，其中免疫异常被认为是最重要的发病因素。溃疡性结肠炎治愈难度大、病程长，经常反复发作，UC的发病率在我国呈升高的趋势。目前UC临床治疗以激素、水杨酸制剂和免疫抑制剂为主，但疗效欠佳，患者易产生耐药性、过敏等不良反应。中药在治疗UC上具有一定优势，避免了传统西药的弊端，在UC的治疗上显示出了广阔前景，临床上以中药灌肠治疗效果较好[1-2]。野茼蒿(Wild chrysanthemum)是菊科茼蒿属一年或二年生草本植物，在我国境内主要分布于广东、海南、四川、云南等地，是一种常用的药食两用植物，含有多种类型化合物，可治疗慢性肠胃炎[3-5]。黏附分子CD62P、CD44、CD54参与炎症反应中的重要环节，在UC的发病过程中起到了重要作用。本实验用2，4，6-三硝基苯磺酸(TNBS)与乙醇混合液诱导UC大鼠模型，将野茼蒿应用于UC大鼠，通过检测其血清中相关黏附分子的变化水平，以期探讨野茼蒿对UC的治疗机制，为其药用价值提供临床依据，现报道如下。, 百拇医药(彭凡珂 冯陈慧 寇力丹)