大鼠心脏能量代谢底物随年龄增长的变化及调节(1)
【摘要】 目的：研究大鼠心肌能量代谢的底物随年龄的变化及调节机制。方法：选用2个月和14个月的雄性SD大鼠，分别为青年对照(young & control，YC)组和老龄对照(aging & control，AC)组。实验末期利用超声心动图测量左心室收缩功能及舒张功能；利用高压液相波谱分析(HPLC)测定心脏ATP的含量；应用Western blot方法检测心肌GLUT-4及CPT-1的表达；用临床生化法检测血Glu、TG、TC、LDL-C、HDL-C、AST、ALT、ALP、BUN、Cr、LDH等指标。结果：实验末期，两组大鼠LVEF比较，差异无统计学意义(P>0.05)。AC组的大鼠心尖ATP含量明显低于YC组，差异有统计学意义(P<0.05)。Western blot检测显示，AC组大鼠GLUT-4与CPT-1表达量较YC组均下降，GLUT-4/CPT-1比值增大(P<0.05)。YC组大鼠血清ALP水平显著高于AC组，差异有统计学意义(P<0.05)；两组大鼠的血清TG、Glu、TC、LDL-C、HDL-C、ALT、AST、Cr、BUN以及LD比较，差异均无统计学意义(P>0.05)。结论：大鼠随年龄增长出现的心肌能量代谢改变，能量产生的减少早于心脏功能的改变。
【关键词】 老龄化； ATP； 葡萄糖转运子-4； 肉碱脂酰转移酶-1
【Abstract】 Objective：To observe the changes and mechanisms of energy metabolism in the aging rat heart.Method：2 months and 14 months male SD rats were chosen and divided into the young & control(YC) group and the aging & control(AC)group.At the end of the experiment using echocardiographic measurements of left ventricular systolic function and diastolic function；phase spectrum analysis using high pressure liquid (HPLC) determination of cardiac ATP expression；application of Western blot method for detection of myocardial GLUT-4
and CPT-1；by clinical biochemical assay of blood Glu，TG， TC，LDL-C，HDL-C，AST，ALT，ALP，BUN，Cr，LDH and other indicators.Result：At the end of the experiment，there was no significant difference in LVEF between the two groups (P>0.05).The content of apical ATP in the AC group was significantly lower than that in the YC group，the difference was statistically significant(P<0.05).Western blot analysis showed that the expression of GLUT-4 and CPT-1 in AC group were lower than those in YC group，the ratio of GLUT-4/CPT-1
was increased(P<0.05).The serum ALP level of YC group was significantly higher than that of AC group，the difference was statistically significant(P<0.05)；the serum TG，Glu，TC，LDL-C，HDL-C，ALT，AST，Cr，BUN and LD in the two groups were not significantly different(P>0.05).Conclusion：The change of myocardial energy metabolism in rats increases with age，and the decrease of energy production is earlier than the change of cardiac function.
【Key words】 Aging； ATP； Glucose transporter-4； Carnitine acyl transferase-1
First-author’s address：Qilu Hospital of Shandong University， Ji’nan 250012，China
doi：10.3969/j.issn.1674-4985.2017.33.007
心臟是一个高耗能的器官，为了保证心脏的泵功能，每天要产生大量的高能代谢产物。因此，心脏的能量代谢直接关系到心脏的结构和功能，而毫无疑问各种心脏疾病和病理改变也会影响到心脏的能量代谢过程[1-4]。目前研究也显示衰老的心脏也出现线粒体结构和功能异常、胰岛素抵抗、脂肪酸代谢障碍等一系列的能量代谢变化。在正常生理状态下，心脏的主要高能产物三磷酸腺苷(adenosine triphosphate，ATP)约有30%来源于葡萄糖有氧氧化，65%来源于脂肪酸有氧氧化。而在心肌肥厚、心肌缺血以及心力衰竭等多种病理状态时，脂肪酸氧化减少，葡萄糖氧化增加，心肌细胞的能量代谢底物转变为以葡萄糖氧化为主，这种代谢底物的重构与胚胎时期心肌的能量代谢底物构成相似，有人称其为“能量代谢胚胎型再演”现象[2-3]。曾有研究报道，在衰老的动物心脏和衰老的人类心脏中均出现脂肪酸氧化减少，并伴有整体氧化代谢水平低下[1-3]，即在衰老的心脏也出现了“能量代谢胚胎型再演”现象。但由于相关的研究非常少，关于老年心脏能量代谢的具体的变化和调节机制仍不清楚。因此，本研究拟对比观察大鼠心脏能量代谢随年龄变化的规律及调节。, 百拇医药(张霞 马丽曼 王妍 刘春 翟艳杰 邢艳秋)
【关键词】 老龄化； ATP； 葡萄糖转运子-4； 肉碱脂酰转移酶-1
【Abstract】 Objective：To observe the changes and mechanisms of energy metabolism in the aging rat heart.Method：2 months and 14 months male SD rats were chosen and divided into the young & control(YC) group and the aging & control(AC)group.At the end of the experiment using echocardiographic measurements of left ventricular systolic function and diastolic function；phase spectrum analysis using high pressure liquid (HPLC) determination of cardiac ATP expression；application of Western blot method for detection of myocardial GLUT-4
and CPT-1；by clinical biochemical assay of blood Glu，TG， TC，LDL-C，HDL-C，AST，ALT，ALP，BUN，Cr，LDH and other indicators.Result：At the end of the experiment，there was no significant difference in LVEF between the two groups (P>0.05).The content of apical ATP in the AC group was significantly lower than that in the YC group，the difference was statistically significant(P<0.05).Western blot analysis showed that the expression of GLUT-4 and CPT-1 in AC group were lower than those in YC group，the ratio of GLUT-4/CPT-1
was increased(P<0.05).The serum ALP level of YC group was significantly higher than that of AC group，the difference was statistically significant(P<0.05)；the serum TG，Glu，TC，LDL-C，HDL-C，ALT，AST，Cr，BUN and LD in the two groups were not significantly different(P>0.05).Conclusion：The change of myocardial energy metabolism in rats increases with age，and the decrease of energy production is earlier than the change of cardiac function.
【Key words】 Aging； ATP； Glucose transporter-4； Carnitine acyl transferase-1
First-author’s address：Qilu Hospital of Shandong University， Ji’nan 250012，China
doi：10.3969/j.issn.1674-4985.2017.33.007
心臟是一个高耗能的器官，为了保证心脏的泵功能，每天要产生大量的高能代谢产物。因此，心脏的能量代谢直接关系到心脏的结构和功能，而毫无疑问各种心脏疾病和病理改变也会影响到心脏的能量代谢过程[1-4]。目前研究也显示衰老的心脏也出现线粒体结构和功能异常、胰岛素抵抗、脂肪酸代谢障碍等一系列的能量代谢变化。在正常生理状态下，心脏的主要高能产物三磷酸腺苷(adenosine triphosphate，ATP)约有30%来源于葡萄糖有氧氧化，65%来源于脂肪酸有氧氧化。而在心肌肥厚、心肌缺血以及心力衰竭等多种病理状态时，脂肪酸氧化减少，葡萄糖氧化增加，心肌细胞的能量代谢底物转变为以葡萄糖氧化为主，这种代谢底物的重构与胚胎时期心肌的能量代谢底物构成相似，有人称其为“能量代谢胚胎型再演”现象[2-3]。曾有研究报道，在衰老的动物心脏和衰老的人类心脏中均出现脂肪酸氧化减少，并伴有整体氧化代谢水平低下[1-3]，即在衰老的心脏也出现了“能量代谢胚胎型再演”现象。但由于相关的研究非常少，关于老年心脏能量代谢的具体的变化和调节机制仍不清楚。因此，本研究拟对比观察大鼠心脏能量代谢随年龄变化的规律及调节。, 百拇医药(张霞 马丽曼 王妍 刘春 翟艳杰 邢艳秋)