沉默Ambra1基因增强大肠癌细胞对5—FU的化疗敏感性(1)
【摘要】 目的：觀察5-FU对沉默Ambra1基因的大肠癌细胞的化疗敏感性影响。方法：将靶向Ambra1基因的siRNA转染至大肠癌细胞HCT15和HCT116，用Real-time PCR和Western blotting方法检测Ambra1 mRNA和蛋白的表达；采用MTS方法检测HCT15和HCT116细胞的活力。结果：HCT15、HCT116细胞转染siRNA-Ambra1-1、siRNA-Ambra1-2后的Ambra1 mRNA表达水平均分别低于siRNA-Ambra1-HCT15-NC、siRNA-Ambra1-HCT116-NC细胞(P<0.05)；HCT15、HCT116细胞转染后的蛋白表达均低于阴性对照；4 μg/mL时，HCT15和HCT116细胞转染siRNA-Ambra1-1、siRNA-Ambra1-2后的细胞克隆形成数均少于0 μg/mL(P<0.05)，而且HCT15、HCT116细胞转染siRNA-Ambra1-1、siRNA-Ambra1-2后的细胞克隆形成数均分别少于siRNA-Ambra1-HCT15-NC、siRNA-Ambra1-HCT116-NC(P<0.05)；HCT15、HCT116细胞转染siRNA-Ambra1-1、siRNA-Ambra1-2后的细胞生存率均分别低于siRNA-Ambra1-HCT15-NC、siRNA-Ambra1-HCT116-NC(P<0.05)；且随着药物浓度增加，已转染了siRNA-Ambra1的细胞生存率均低于阴性对照组。结论：沉默Ambral基因可提高大肠癌细胞对5-FU的化疗敏感性。
, 百拇医药
【关键词】 大肠癌； siRNA； Ambra1； 化疗敏感性； 5-FU
siRNA-Ambra1 Enhances Chemosensitivity of Human Colon Cancer Cells for 5-FU/WANG Liantao，CHENG Haichun，WEN Huasheng，et al.//Medical Innovation of China，2018，15(23)：0-043
【Abstract】 Objective：To observe the effect of 5-FU on chemosensitivity of colorectal cancer cells silencing Ambra1 gene.Method：siRNA targeting Ambra1 gene was transfected into colorectal cancer cells HCT15 and HCT116.The expressions of Ambra1 mRNA and protein were detected by Real-time PCR and Western blotting.The viability of HCT15 and HCT116 cells was detected by MTS.Result：The expression levels of Ambra1 mRNA in HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were lower than those of siRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05).The protein expression of HCT15 and HCT116 cells after transfection were lower than those of negative control.When 4 μg/mL，the cell clone formation number of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were less than 0 μg/mL(P<0.05)，and the cell clone formation number of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were less than those of siRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05).The cell survival rates of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were lower than those of iRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05)，with the increase of drug concentration，the survival rate of the transfected siRNA-Ambra1 cells was lower than that of the negative control group.
Conclusion：siRNA-Ambralcan enhance chemotherapy sensitivity of colon cancer cells to 5-FU.
【Key words】 Colon cancer； Small interfering RNA； Ambra1； Chemotherapy sensitivity； 5-FU, http://www.100md.com(王连涛 成海春 温华生)
, 百拇医药
【关键词】 大肠癌； siRNA； Ambra1； 化疗敏感性； 5-FU
siRNA-Ambra1 Enhances Chemosensitivity of Human Colon Cancer Cells for 5-FU/WANG Liantao，CHENG Haichun，WEN Huasheng，et al.//Medical Innovation of China，2018，15(23)：0-043
【Abstract】 Objective：To observe the effect of 5-FU on chemosensitivity of colorectal cancer cells silencing Ambra1 gene.Method：siRNA targeting Ambra1 gene was transfected into colorectal cancer cells HCT15 and HCT116.The expressions of Ambra1 mRNA and protein were detected by Real-time PCR and Western blotting.The viability of HCT15 and HCT116 cells was detected by MTS.Result：The expression levels of Ambra1 mRNA in HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were lower than those of siRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05).The protein expression of HCT15 and HCT116 cells after transfection were lower than those of negative control.When 4 μg/mL，the cell clone formation number of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were less than 0 μg/mL(P<0.05)，and the cell clone formation number of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were less than those of siRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05).The cell survival rates of HCT15 and HCT116 cells transfected with siRNA-Ambra1-1 and siRNA-Ambra1-2 were lower than those of iRNA-Ambra1-HCT15-NC and siRNA-Ambra1-HCT116-NC cells respectively(P<0.05)，with the increase of drug concentration，the survival rate of the transfected siRNA-Ambra1 cells was lower than that of the negative control group.
Conclusion：siRNA-Ambralcan enhance chemotherapy sensitivity of colon cancer cells to 5-FU.
【Key words】 Colon cancer； Small interfering RNA； Ambra1； Chemotherapy sensitivity； 5-FU, http://www.100md.com(王连涛 成海春 温华生)
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