CCK-8法检测兔筋膜成纤维细胞增殖活性之最佳测试条件研究(1)
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【摘要】目的研究CCK-8法检测兔筋膜成纤维细胞(RFFBs)增殖活性的最佳测试条件。方法以兔筋膜作为成纤维细胞原代培养组织，利用传代至P3的成纤维细胞，对比CCK-8法在不同孵育时间及不同检测波长条件下吸光光度值的差异，研究CCK-8法检测兔筋膜成纤维细胞增殖活性的最佳检测时间及最佳检测波长。结果CCK-8法检测兔筋膜成纤维细胞增殖活性的最佳检测时间为加入CCK-8试剂孵育后的4 h，最佳检测波长为450 nm。结论CCK-8法在孵育4 h后及酶标仪450 nm波长下检测兔筋膜成纤维细胞的灵敏度最高。
【关键词】CCK-8法；成纤维细胞；兔；增殖活性；测试条件
An experimental research on the best test conditions of CCK-8 method detecting rabbit fascia fibroblasts(RFFBs) proliferation activity XIN Pei-cheng,YANG Sheng,LU Jian-min,ZHAO De-wei.Affiliated Zhongshan Hospital of Dalian University,Dalian 116001,China 【Abstract】ObjectiveTo research the best test conditions of CCK-8 method detecting rabbit fascia fibroblasts(RFFBs) proliferation activity.MethodsWith rabbit fascia tissue as fibroblasts former generation training organization,using the fibroblasts of P3 generation，comparing the difference of OD value between different kinds of test times or different kinds of test wavelengths ......
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