高效液相色谱法测定人血浆中帕珠沙星浓度
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【摘要】目的建立HPLC-紫外法检测人血浆中帕珠沙星浓度的方法。方法采用25%高氯酸溶液沉淀蛋白，分析柱为CNWSILC18(4.6 mm×100 mm)，流动相为0.02 mol/L磷酸二氢钠溶液-乙腈-三乙胺(86∶13.5∶0.5)，pH=3.5，紫外测定波长248 nm。结果帕珠沙星浓度在0.08~30.96 mg/L呈线性关系，r=0.9998(n=6)；萃取回收率高于88%，方法回收率98%~101%，日间日内精密度均小于6%。在-40 ℃冰箱保存15 d或冻融2次稳定性较好。结论本方法简便灵敏，适合用来检测血浆中帕珠沙星浓度。
【关键词】帕珠沙星；高效液相色谱；紫外检测
High performance liquid chromatography (HPLC) to human plasma concentration of pearl sand pato XU Jian-ping.The First Peoples Hospital of Hengyang,Hengyang 421002,China
【Abstract】ObjectiveTo establish HPLC ultraviolet method to detect human plasma concentrations of pearl sand pato method.MethodsUsing 25% high chlorine acid solution protein deposition, analysis for CNWSILC18 (4.6 mm column 100mm),mobile phase (mol/L for 0.02 sodium dihydrogen phosphate solution-acetonitrile-three procedures (86∶13.5∶0.5),pH= 3.5,uv wavelength 248nm determination.ResultsPalmer bead uptake in 30.96 0.08-concentration of the linear mg/L,r=0.9998(n= 6);Extraction recovery higher than 88%, method,daytime 101% recovery98-are less than 6% within precision ......
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