肺炎支原体核酸荧光PCR法与血清 被动凝集法的比对分析
荧光PCR检测,血清学抗体检测,被动凝聚
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摘要：目的：探讨肺炎支原体核酸荧光PCR法与血清学抗体检测—被动凝集法的比对关系。方法：将有咳嗽、低热、X线胸片检查有肺纹理增粗的急性上呼吸道感染的0—12岁的患儿700例分别获取咽拭子进行核酸荧光PCR法检测和静脉抽血进行血清学抗体检测。结果：上述700例患儿中，肺炎支原体核酸荧光PCR法阳性率和灵敏度在0—4岁阶段明显高于血清学检验，4—12岁阶段阳性率低于血清学检验但特异性明显高于血清学检验。结论：核酸荧光PCR法具有早期、快速、特异等优点，血清被动凝聚法灵敏度和特异性都较低，特别是婴幼儿免疫系统还未发育完善，产生抗体的速度较慢，抗体量不够，易产生假阴性。
关键词：肺炎支原体 荧光PCR检测 血清学抗体检测 被动凝聚
Comparative Analysis OfMycoplasma PneumoniaeNucleic Acid Fluore
-scentPCRAnd SerumPassive Agglutination
Shu MeilianDepartment OfHunanProvinceYuanling County Hospital Of Traditional Chinese Medicine
Abstract:Objective: Discussion oninspection/Mycoplasma pneumoniaenucleic acid fluorescentPCRandserum antibody ofPassive AgglutinationMethod relations.Method: There are 700cases of cough,fever,chest X-rayexamination isthe lung texture increasesacute upper respiratory tractInfectionofcoarseaged 0 to12years oldchildren,who got thepharynx swabs ofnucleic acidfluorescent PCR detectionandvenous bloodserumanti-body detection ......
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