不同方法测定血红蛋白溶液中总蛋白量的对比分析
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摘要:目的 采用三种方法对含有大量血红蛋白溶液的样本进行总蛋白量的测定，并进行对比，确定一种可行且简单的测定血红蛋白溶液中总蛋白量的方法。方法 分别采用BCA法、Bradford法和Lowry法测定溶液总蛋白量，并采用HiCN法测定溶液中血红蛋白的含量。比较不同方法测定数据的差异。结果 5份样本用BCA测得总蛋白量为76.33g/L，Bradford法测得值为179.59g/L，Lowry法测得值为22.81g/L，HiCN法测得血红蛋白量为133.65g/L。结论 Bradford法是一种可行且重复性好的测定血红蛋白溶液中总蛋白量的方法，BCA法和Lowry法的测定值明显低于实际值。
关键词 血红蛋白溶液 总蛋白量
The Compsrison of Different Handwork Methods on Measuing the Total Protein Concentration of the Hemoglobin Solution.Cai Ming-cui.The Blood Center of Liangshan,Xichang 615000,Sichuan,China.
【Abstract】Objective Three methods commonly used do determine the protein concentration were taken to measure the total protein of the solution containing stroma-free hemoglobin. And we tried to confirm a feasible way on measuring the total protein concentration through comparison of different methods.Methods The BCA, Bradford and Lowry methods were used to determine the total peotein, and the HiCN method was exploited to measure the level of hemoglobin.Results The average concentration of the total protein of 5 samples was 76.33g/L by BCA,179.59g/L by Bradford, and 22.81g/L by Lowry. The average concentration of hemoglobin by HiCN method was 133.65g/L.Conclusion The Bradford method was feasible and precise to determine the total protein concentration of hemoglobin solution. The results of BCA and Lowry methods were obviously lower than that of Bradford method and even the level of hemoglobin. ......
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