自拟骨伤方I号消肿镇痛及成骨作用的实验研究(1)
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【摘要】目的：研究骨伤方Ⅰ号对骨折的治疗作用，并对其治疗的机理作初步探讨。方法：消肿和镇痛实验中，按随机数字表法等分4组，空白组、自拟骨伤方Ⅰ号高、中、低剂量组,灌胃给药。采用二甲苯诱发小鼠耳廓肿胀法研究其抗炎作用;采用热板法致小鼠舔后足研究其镇痛作用。在成骨实验中：制作大兔骨折模型，术后次日灌胃给予骨伤方Ⅰ号，分别于给药后14，28d，测定骨折处骨矿物密度，同时测定血小板。结果：骨伤方Ⅰ号组显著降低二甲苯致小鼠耳肿胀的肿胀率,增加小鼠的痛阈值。给药14d、28d后，骨伤方Ⅰ号高、中剂量组的骨密度增加，与空白组比较有显著性差异(P<0.05)；给药14d、28d后可降低血小板聚集率。结论：自拟骨伤方Ⅰ号具有显著的消肿及镇痛作用，能加快骨矿沉积速率，提高骨密度，改善血液流变学。
【关键词】自拟骨伤方Ⅰ号；消肿；镇痛；骨折；骨密度
Experimental study of traumatology&orthopedicsI in the treatment of detumescence analgesia and promoting fracture healing efficacy
Chen Jinzhao Cai Dongling Liu Chunlan Chen Xiaofeng
Panyu TCM Hospital,Guangzhou,511400
【Abstract】Objective:To investigate the therapeutic effect of traumatology&orthopedicsI on fracture and explore its mechanism.Methods:detumescence and analgesia experiment:The experimental animals which respectively intragastrac administration were divided into normal group, high dose group, middle dose group and low dose group. The methods included hot-plate procedure and the ear swelling induced with xylene.Besides, The bone mineral density (BMD) and the parameters of hemorheology in the fractured radius were also observed in 14 and 28 days after treatment. Results:The traumatology&orthopedicsI can reduce engorgement and increase the pain threshold. The high and middle dose group can increase the BMD and reduce platelet aggregation, which had obvious variance compared with the control group(P<0.05). Conclusion: The traumatology&orthopedicsI have the effect ofdetumescence and analgesia,can improve and accelerate fracture healing in the catagmatic rabbits by increasing BMD and improving blood rheology.
【Keyword】Traumatology&orthopedicsI; detumescence; analgesia; facture; Bone mineral density
骨折愈合是个复杂的过程,影响因素很多。自从1959年刘润田[1]首次报道中药促进骨折愈合的动物研究以来,人们通过从不同的角度进行了深入的研究,并得出较为可靠的结论,显示中医药促进骨折愈合的生命力。广州市番禺区中医院研制的骨伤方I号是骨折治疗后结合辩证分期的内服中药, 为进一步探讨其消肿、镇痛及促进骨折愈合的作用机理,本实验通过动物实验为该药的临床应用提供药理学依据,现将其结果总结报告如下：
1材料与仪器
1.1实验动物昆明小鼠,雌雄兼用,体质量18~22g, 大耳白家兔，雄性，体重2~2.5kg，均购自广东省医学实验动物中心,合格证号:粤监证字2008A015。
1.2药物与试剂供试品: 自拟骨伤方I号,由广州市番禺区中医院提供,由金银花、连翘、田七、甘草、防风、蝉蜕、赤芍、羚羊角、薏苡仁等组成,并配制成高、低剂量(0.12、0.03g/mL);试剂:二甲苯(天津市科密欧化学试剂有限公司生产,批号: 20070523); 3 ......
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