糖尿病心肌病与早期扩张性心肌病心肌线粒体通透性转运孔开放的对比观察(1)
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【摘 要】 目的：观察糖尿病心肌病与早期扩张性心肌病心肌线粒体通透性转运孔开放程度异同。方法：30只Wistar大鼠随机分成对照组、糖尿病组及扩心病组。造模成功，提取心肌线粒体，检测线粒体PTP开放、膜电位及Ca2+转运。结果：MPTP开放检测吸光值糖尿病组最高，与对照组及扩心病组比较均有显著性差异，扩心病组与对照组比较，有显著性差异；Ca2+转运检测吸光值及膜电位Rh123荧光值糖尿病组与对照组比较，有显著性差异，扩心病组与对照组比较，有显著性差异。结论：糖尿病心肌病与早期扩张性心肌病线粒体MPTP呈高开放状态，前者显著高于后者，MPTP呈高开放状态可能是糖尿病心肌病与早期扩张性心肌病在线粒体能量代谢方面共同病理生理机制。
【关键词】 糖尿病心肌病；扩张性心肌病；线粒体通透性转运孔
Diabetic cardiomyopathy and dilated cardiomyopathy early myocardial mitochondrial
permeability transition pore opening Comparative study
【Abstract】Objective：Observation of early diabetic cardiomyopathy and dilated cardiomyopathy myocardial mitochondrial permeability transition pore similarities and differences between the degree of openness。Methods：30 Wistar rats were randomly divided into control group, diabetes, expanding heart disease group ，Diabetic cardiomyopathy and dilated cardiomyopathy early modeling success, Detected mitochondrial PTP opening and membrane potential and Ca2 + transport。Results：MPTP opening detect the highest absorbance value of the diabetic group compared with the control group and extend heart disease group were significantly different, expanding heart disease group and control group, there is a significant difference ；Detection of Ca2 + transport absorbance and Membrane potential, Rh123 fluorescence diabetic group and control group, there is a significant difference, expanding heart disease group and control group, there is a significant difference。Conclusion Diabetic cardiomyopathy and dilated cardiomyopathy early mitochondrial MPTP showed a high open state, the former was significantly higher than the latter。Diabetic cardiomyopathy and dilated cardiomyopathy early in the mitochondrial energy metabolism that is a common pathophysiologic mechanism。
【Key words】Diabetic cardiomyopathy; dilated cardiomyopathy; MPTP
线粒体膜通透转运孔(mitochon-drialpermeability transition pore, MPTP)是线粒体内外信息交流的中心枢纽,其开放状态决定着线粒体功能的发挥。本研究旨在观察MPTP在糖尿病心肌病(diabetic cardiomyopathy,DC)及扩张性心肌病实验大鼠中(dilated cardiomyopathy,DCM)开放高低的差异，以探讨MPTP在两者病理生理机制中作用的异同。
1 材料和方法 ......
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