脐血铅浓度与新生儿端粒长度的相关性(3)

http://www.100md.com 2014年3月15日 赵艳芳 曲宝明 刘文东 马海燕

第1页

    参见附件。

     Reverse primer，5’-GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT-3’；primer sequences of 36B4 gene was： Forward primer，5’-CAGCAAGTGGGAAGGTGTAATCC-3’；Reverse primer，5’-CCCATTCTATCATCAACGGGTACAA-3’. Cycling conditions (for both telomere and 36B4)were：10min at 95℃，followed by 40 cycles of 95℃，for 5s， 56℃for 30s，and 72℃ for 60s.

    1.2.2 Make standard curve and calculate telomere length Select one sample to dilute (dilution factor is five) according to the ratio of equality as the standard preparation ......

    http://www.100md.com/html/paper/2095-0616/2014/06/01-2.htm

您现在查看是摘要介绍页，详见PDF附件。