袁柳媚 卢小叶 夏云 戴雅婷 范宓 娄必丹

    [摘要]线栓法制备大鼠大脑中动脉闭塞脑缺血模型因无需开颅，并且具有梗死体积相对稳定、可重复性高、可操作性强等特点，目前在国内应用广泛。该模型是公认的标准局灶性脑缺血动物模型最理想的模型之一。笔者结合实践经验和既往文献报道，从大鼠的选择、模型制备以及术后死亡、并发症的原因分析与处理等多个方面分享线栓法制备大鼠大脑中动脉闭塞脑缺血模型的体会。通过不断改进术中操作方法、及时有效地处理术后并发症引起的相关症状，提高了大鼠的术后存活率。

    [关键词]线栓法;大脑中动脉闭塞;脑缺血;动物模型;大鼠

    [中图分类号]R-332;R743? [文献标识码]A? [文章编号]2095-0616(2022)02-0007-04

    Experience of the establishment of cerebral ischemia model of middle cerebral artery occlusion in rats using suture-occluded method

    YUAN Liumei1? LU Xiaoye1? XIA Yun2? DAI Yating1? FAN Mi1? LOU Bidan2

    1. Hunan University of Traditional Chinese Medicine，Hunan，Changsha 410007，China;2. The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine，Hunan，Changsha 410007，China

    [Abstract]The cerebral ischemia model of middle cerebral artery occlusion in rats prepared by suture- occluded method is widely used in China because it does not require craniotomy and has the characteristics of relatively stable infarct volume ......