Effects of Yulangsan polysaccharides on BDNF pathway in the dentate gy(5)
Effects of Yulangsan polysaccharides on BDNF pathway in the dentategyrus of the hippocampus of mice with chronically stressed
①The slices were incubated overnight at 4℃ with mouse monoclonal antibody against TrkB (diluted 1：200； Santa Cruz， Inc) and rabbit polyclonal antibody against BDNF (diluted 1：100；Beijing Biosynthesis Biotechnology Co.， Ltd.).Neurons cells ， in which the cytoplasm was stained yellowish， were considered to be positive cells.②Sections were incubated with CREB or p-CREB antibodies (diluted in PBS 1：200 for CREB and p-CREB； Santa Cruz ......
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