【摘 要】目的：探讨梗阻性黄疸诱导小鼠肝细胞损伤的病理机制。方法：24只雄性小鼠随机分为胆总管结扎组(BDL组)和假手术组(SO组)，分别于手术后2天，28天取血及肝组织(n=6)，检测血清胆红素(TB)、碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST)水平变化。应用HE染色、Western Blot、免疫组化染色进行评估。结果：与SO组相比，BDL组小鼠TB、ALP、ALT、AST术后显著升高，急性期RIP3表达升高，Cleaved caspase3表达未见明显升高；慢性期RIP3及Cleaved Caspase3均显著升高。结论：急性胆汁淤积诱导的肝损伤以肝细胞程序性坏死为主，肝细胞凋亡少见；慢性胆汁淤积诱导的肝损伤以肝细胞程序性坏死和肝细胞凋亡为主。

    【Abstract】Objective： To investigate the pathological mechanism of hepatocyte injury induced by obstructive jaundice in mice. Methods： 24 male mice were randomly divided into bile duct ligation group (group BDL) and sham operation group (group SO). Blood and liver tissue (n=6) were taken at 2 days after operation and 28 days after operation. Serum bilirubin (TB) ......