急慢性胆道梗阻小鼠肝细胞的死亡方式(1)
【摘 要】目的:探讨梗阻性黄疸诱导小鼠肝细胞损伤的病理机制。方法:24只雄性小鼠随机分为胆总管结扎组(BDL组)和假手术组(SO组),分别于手术后2天,28天取血及肝组织(n=6),检测血清胆红素(TB)、碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST)水平变化。应用HE染色、Western Blot、免疫组化染色进行评估。结果:与SO组相比,BDL组小鼠TB、ALP、ALT、AST术后显著升高,急性期RIP3表达升高,Cleaved caspase3表达未见明显升高;慢性期RIP3及Cleaved Caspase3均显著升高。结论:急性胆汁淤积诱导的肝损伤以肝细胞程序性坏死为主,肝细胞凋亡少见;慢性胆汁淤积诱导的肝损伤以肝细胞程序性坏死和肝细胞凋亡为主。
【Abstract】Objective: To investigate the pathological mechanism of hepatocyte injury induced by obstructive jaundice in mice. Methods: 24 male mice were randomly divided into bile duct ligation group (group BDL) and sham operation group (group SO). Blood and liver tissue (n=6) were taken at 2 days after operation and 28 days after operation. Serum bilirubin (TB) ......
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