Intranasal Exposure to Bacterial Superantigens Induces Airway Inflammation in HLA Class II Transgenic Mice
http://www.100md.com
感染与免疫杂志 2006年第2期
Department of Immunology
Division of Allergic Diseases Research
Division of Infectious Diseases, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, Minnesota 55905
ABSTRACT
Staphylococcus aureus is widely prevalent in the nasopharynges of healthy individuals (carriers) but can also cause serious infections. S. aureus can elaborate a variety of superantigen exotoxins in "carrier" or "pathogenic" states. Streptococcus pyogenes can also colonize the nasopharynx and elaborate superantigens. Unlike the acute effects of superantigen exotoxins absorbed through the gut or vaginal mucosa, little is known regarding the pathogenesis of superantigens entering through the intranasal route. In the current study, we evaluated the local and systemic effects of staphylococcal enterotoxin B (SEB) and streptococcal pyrogenic exotoxin A (SPEA) delivered through the intranasal route. Superantigens were administered intranasally on multiple occasions, and experimental animals were sacrificed on day 8 for experimental analyses. SEB-induced airway inflammation was more pronounced for HLA-DR3 transgenic mice than for BALB/c mice, consistent with bacterial superantigens binding more efficiently to human than murine major histocompatibility complex class II. The nature of the airway inflammation in HLA-DR3 mice was determined by the concentration of SEB applied intranasally. Low concentrations (20 ng) induced eosinophilic airway inflammation as well as eosinophil degranulation, whereas intranasal exposure to higher concentrations (2,000 ng) resulted in neutrophilic airway inflammation, permanent airway destruction, toxic shock, and mortality. SEB-induced eosinophilic inflammatory response was enhanced in signal transducer and activator of transcription (STAT)-4-deficient HLA-DQ8 transgenic mice with defective interleukin-12 signaling. Intranasal administration of SPEA induced airway inflammation and systemic immune activation in HLA-DQ8 transgenic mice. In conclusion, repeated chronic intranasal exposure to bacterial superantigens causes airway inflammation and systemic immune activation.
INTRODUCTION
Bacterial superantigens are a family of microbial polypeptide exotoxins capable of inducing strong proliferation of T lymphocytes even at extremely low concentrations (21, 63). They are the most potent T-cell mitogens known to humankind. Mechanistically, unlike conventional antigens which bind to the peptide-binding groove of major histocompatibility complex (MHC) class II molecules after undergoing a series of processing steps, superantigens bind directly to MHC class II molecules outside of the peptide-binding groove without undergoing processing. While exogenous antigens presented by MHC class II molecules activate CD4+ T cells (34), MHC class II-bound superantigens activate both CD4+ and CD8+ T cells. While conventional antigens activate specific T cells by interacting with both and chains of T-cell receptor (TCR) molecules (83), superantigens activate T cells by binding directly to the variable region of the TCR chain (and in rare cases TCR V families [69]), independent of their antigen specificities (56). Superantigens thus activate 30 to 50% of the total T-cell pool, whereas conventional antigens activate approximately 1 in 104 to 1 in 106 T cells (56). By virtue of their capacity to induce strong immune activation, superantigens can cause a variety of illnesses, ranging from acute food poisoning to toxic shock syndrome (21, 51), and have even been implicated in some autoimmune diseases (71, 108).
Bacterial superantigens are produced primarily by Staphylococcus aureus and Streptococcus pyogenes (71). S. aureus is ubiquitous in nature (7, 12, 58), and humans are natural carriers (primarily the nasal passage) (12, 68). While more than half of the pathogenic isolates produce one or more superantigen exotoxins (6, 28, 104), even strains isolated from asymptomatic carriers can produce superantigens (6, 28, 35, 49, 64, 103). The nasopharynx is also the site of colonization by group A, group C, and group G streptococci. Among these, S. pyogenes is considered the most pathogenic and elaborates a variety of superantigen exotoxins (77). Pathogenic S. pyogenes strains producing pyrogenic exotoxins have been isolated from asymptomatic schoolchildren living in areas with reported outbreaks of invasive streptococcal disease (15). While group C and group G streptococci are generally considered commensals, recent studies have demonstrated that they too can harbor genes encoding superantigen exotoxins (41, 45, 85). In addition to the presence of genes encoding bacterial superantigens in these isolates, recent studies have demonstrated the presence of transcripts encoding bacterial superantigens following experimental infection (102) and exotoxins themselves in patients with staphylococcal nasal carriage (89).
Given the presence of S. aureus and group A, group C, and group G streptococci in the human nasopharynx in either the carrier state or the disease state, it is probable that the respiratory tract is exposed to their products, including superantigens (3, 31, 40, 48, 70). It has been shown that staphylococcal protein A (30), cell wall peptidoglycan and lipoteichoic acid (54), and bacterial DNA containing unmethylated CpG motifs (88) can cause inflammation of the airways and lungs following direct exposure through the airway. However, the pathological effects of superantigen exotoxins produced by S. aureus and S. pyogenes on the respiratory system following direct exposure through the nasopharyngeal route have not been explored. As bacterial superantigens are potent T-cell mitogens, they have the potential to cause airway and lung inflammation through the same mechanisms by which they cause other diseases. While there are some indications that bacterial superantigens play a role in airway inflammation, including with asthma (4, 36, 37, 79) and chronic obstructive pulmonary disease (80), a definite role for bacterial superantigens in airway and/or lung inflammation has not yet been established using an appropriate animal model. Not only does this have immense clinical relevance, but the looming threat of the deployment of aerosolized superantigens as biological weapons necessitates elucidation of the effects of superantigen delivered directly to the nasal passage (33, 44, 60). In the current study, we evaluated the concentration-dependent effects of bacterial superantigen on the airways and lungs following intranasal exposure using a mouse model. Since bacterial superantigens bind poorly to murine MHC class II molecules (52), we used mice lacking endogenous class II but transgenically expressing high-affinity human HLA class II molecules (16, 73-76, 94, 110). Results of this study offer novel insight into the pathogenesis of airway inflammation.
MATERIALS AND METHODS
Mice. HLA-DR3 transgenic mice expressing functional HLA-DRA10101 and HLA-DRB10301 transgenes on the complete mouse MHC class II-deficient background (AEo) (14) have been previously described (74). Previously described HLA-DQ8 transgenic mice expressing HLA-DQA10301 and HLA-DQB10302 transgenes (74) were also studied. Signal transducer and activator of transcription (STAT)-4-deficient, HLA-DQ8 transgenic mice were generated by William Stohl (University of Southern California Keck School of Medicine, Los Angeles, CA). T-cell responses in these HLA transgenic mice are restricted only by the transgenic HLA class II molecules (96). BALB/c mice were from The Jackson Laboratory (Bar Harbor, ME). Mice were bred within the barrier facility of Mayo Clinic Immunogenetics Mouse Colony (Rochester, MN) and moved to a conventional facility after weaning. All experiments were approved by the Institutional Animal Care and Use Committee.
Superantigen and animal challenge. Staphylococcal enterotoxin B (SEB) and streptococcal pyrogenic exotoxin A (SPEA) (Toxin Laboratories, Sarasota, FL) were dissolved in sterile, endotoxin-free phosphate-buffered saline (PBS) and stored at –80°C. For intraperitoneal challenge, mice received the indicated concentrations of SEB in 200 μl of PBS. For intranasal challenge, mice were anesthetized with 2,2,2-tribromoethanol (Avertin; Aldrich Chemical, Milwaukee, WI), at a dose adjusted so that pedal reflexes were absent. Subsequently, mice were held in an upright position and 50 μl PBS alone or different concentrations of superantigens dissolved in 50 μl PBS were instilled into the nostrils. Mice were laid in a supine position until they recovered from anesthesia.
Measurement of AHR. Airway hyperreactivity (AHR) was assessed on the eighth day after the first intranasal SEB application by methacholine-induced airflow obstruction in conscious mice with a whole-body plethysmograph (Buxco Electronics, Troy, NY), using a previously described procedure (50). Pulmonary airflow obstruction was measured by enhanced pause (Penh) as follows: Penh = (Te/RT – 1) x (PEF/PIF), where Penh is enhanced pause (dimensionless), Te is expiratory time, RT is relaxation time, PEF is peak expiratory flow (ml/s), and PIF is peak inspiratory flow (ml/s). Penh, minute volume, tidal volume, and breathing frequency were obtained from chamber pressure, measured with a transducer connected to preamplifier modules, and analyzed by system software (all from Buxco Electronics). To measure methacholine responsiveness, mice were exposed to PBS for 2 min, followed by an incremental dosage (3 to 50 mg/ml) of aerosolized methacholine (freshly prepared in PBS); Penh was monitored for each dose of methacholine. Results for PBS and methacholine were expressed as percentage of baseline Penh values before PBS exposure.
Collection of BAL fluid. Immediately after AHR was measured, mice were injected intraperitoneally with a lethal dose of 2,2,2-tribromoethanol. The trachea was cannulated, the lungs were lavaged twice with 0.5 ml of PBS, and the fluids were pooled. After centrifugation, the supernatant was collected and stored at –80°C. The cells were resuspended and counted using a hemacytometer. Bronchoalveolar lavage (BAL) fluid cell differentials were determined by Wright-Giemsa staining; 200 cells were differentiated using conventional morphological criteria. Concentrations of gamma interferon (IFN-) in BAL fluid supernatants were measured using an enzyme-linked immunosorbent assay kit as directed by the manufacturer (R&D Systems, Minneapolis, MN).
Flow cytometry. Mononuclear cells from lymphoid organs and BAL fluid were stained with fluorescein isothiocyanate-conjugated anti-V6 or anti-V8, phycoerythrin-conjugated CD4, and peridinin chlorophyll protein-conjugated CD8. Alternatively, B cells and macrophages were analyzed using B220 and Mac-1 antibodies, respectively. All antibodies were from BD Pharmingen (San Diego, CA).
Histopathology and immunohistochemistry. After BAL fluid collection, the lungs were fixed in 10% formalin and embedded in paraffin. Six-millimeter sections were stained with hematoxylin and eosin for histopathological evaluation. For demonstrating eosinophils, processed formalin-fixed tissue sections were stained with rabbit anti-mouse major basic protein (MBP), as previously described (50).
Statistics. Statistical evaluation was carried out using InStat (version 3.0; GraphPad Software, Inc., San Diego, CA). Student's t test or analysis of variance (ANOVA) was performed, depending on the number of groups in each experiment.
RESULTS
Response to SEB in HLA-DR3 transgenic mice versus response in BALB/c mice. Intraperitoneal challenge with 10 μg of SEB resulted in a significantly higher (P < 0.05, unpaired Student's t test) expansion of CD4+ and CD8+ T cells bearing SEB-reactive TCR V8, but not the nonreactive TCR V6, in the spleens of HLA-DR3 transgenic mice than in spleens of BALB/c mice (Fig. 1A). Further, as depicted in Fig. 1B, the CD4 CD8 double-positive thymocytes in HLA-DR3 mice underwent a significant reduction compared to those in BALB/c mice (P < 0.001, unpaired Student's t test). Two injections of 10 μg SEB 48 h apart resulted in 100% mortality in HLA-DR3 mice (mortality, four of four mice), whereas all of the BALB/c mice tested survived this challenge (mortality, zero of four mice). These results confirm that HLA class II transgenic mice are better models for studying the immune response to superantigens than are conventional mouse strains.
Intranasal delivery of SEB results in inflammation of the airways and lungs in HLA-DR3 transgenic mice. To compare the direct effects of SEB on the airways of HLA-DR3 and BALB/c mice, we challenged these two strains with the indicated concentrations of SEB by the intranasal route (Fig. 2). Intranasal delivery of SEB resulted in a significantly higher accumulation of macrophages, eosinophils, neutrophils, and lymphocytes in BAL fluid of HLA-DR3 mice than in BAL fluid of BALB/c mice (P < 0.05, unpaired Student's t test), even though the latter were challenged with 2.5 times the dose used for the former. Only alveolar macrophages were detected in BAL fluid of PBS-challenged mice from either group. Since SEB elicited more-severe airway inflammation in HLA-DR3 mice than in BALB/c mice, the former were used in subsequent studies.
Concentration-dependent variations in airway responses to SEB in HLA-DR3 transgenic mice. We evaluated the effect of SEB concentration (20, 200, or 2,000 ng) on airway inflammation by challenging HLA-DR3 mice with increasing concentrations of SEB and measuring airway responses on the eighth day. Mice administered 20 ng of SEB did not show any visible signs of distress and appeared healthy throughout the study. However, mice administered 2,000 ng of SEB exhibited clear signs of distress, including ruffled fur, labored breathing, listlessness, and weight loss. While the body weights of the mice in all groups were comparable on day 0, the body weights of the PBS group was 25.25 ± 1.70 g (n = 9) on day 8, while that of the SEB 2,000-ng group was 16.6 ± 1.7 g (n = 4) (P = 0.0002, unpaired Student's t test).
While no mortality was noted after the first dose of intranasal SEB delivery for any of the groups studied, high mortality was observed after the second or third dose of intranasal SEB delivery for the 200-ng (mortality, 4/10 mice) and 2,000-ng (mortality, 6/10 mice) groups. Mortality was not observed during or immediately after administration of SEB but rather occurred during the ensuing 24 to 48 h. Thus, airway exposure to low concentrations of SEB did not cause any apparent clinical signs, while higher concentrations of SEB caused signs consistent with toxic shock and were associated with mortality.
We evaluated AHR in mice that had been administered SEB or PBS intranasally by measuring the methacholine-induced airway changes at the termination of the experiment (i.e., on day 8). Mice that received PBS and 20 ng/dose of SEB had comparable baseline AHRs as measured by Penh. However, airway resistance in the SEB 20-ng group showed definite changes in response to increasing concentrations of inhaled methacholine compared to airway resistance in the PBS-treated group (P = 0.25, 0.045, 0.05, and 0.313 at 3, 6, 12, and 25 mg of methacholine, respectively; unpaired Student's t test) (Fig. 3A). On the other hand, mice that received 200 and 2,000 ng of SEB had significantly higher baseline AHRs than mice that received intranasal PBS (P < 0.05, ANOVA). AHR changed little following methacholine challenge in these groups. This indicated that while a low concentration of SEB (20 ng) was associated with reversible AHR, higher concentrations of SEB (200 and 2,000 ng) were associated with permanent airway changes and showed little methacholine-induced change (Fig. 3A), suggesting a concentration-dependent effect of SEB on certain airway responses.
We next studied the effect of variation in SEB concentration on the types of leukocytes recruited to the airways by analyzing the BAL fluid (Fig. 3B). Lower concentrations of SEB recruited more eosinophils into BAL fluid than did higher concentrations of SEB (P < 0.01 for PBS versus SEB 20-ng groups; P < 0.05 for PBS versus SEB 200-ng groups as well as SEB 20-ng versus 2,000-ng groups; one-way ANOVA). Higher concentrations of SEB were associated with more neutrophil recruitment into BAL fluid than were lower concentrations (P < 0.05 for all SEB groups compared to the PBS group; P < 0.001 for SEB 2,000-ng versus 20-ng or 200-ng groups; one-way ANOVA). The eosinophil-to-neutrophil ratios were 1.6, 0.75, and 0.03 for 20, 200, and 2,000 ng/dose of intranasal SEB, respectively. Macrophages were more abundant at lower SEB concentrations (P < 0.01 for PBS versus SEB 20-ng groups as well as PBS versus SEB 200-ng groups; P < 0.05 for SEB 20-ng versus SEB 2,000-ng groups; one-way ANOVA). An inverse concentration effect was also seen for lymphocyte recruitment. Lymphocytes were more abundant at lower SEB concentrations (Fig. 3B and C) (P < 0.01 for PBS versus SEB 20-ng groups; P < 0.05 for PBS versus SEB 200-ng groups as well as SEB 20-ng versus SEB 2,000-ng groups; one-way ANOVA). Together, these results suggest that bacterial superantigens are capable of inducing concentration-dependent AHR and that the characteristics of inflammatory-cell recruitment vary in a SEB concentration-dependent manner. We also quantified the levels of IFN- in BAL fluid from superantigen- or PBS-challenged mice (Fig. 3D). IFN- levels were significantly higher in SEB 200-ng and SEB 2,000-ng groups than in either PBS or SEB 20-ng groups (P < 0.05).
To further evaluate the pathological changes in the lungs following SEB challenge, paraffin-fixed lung sections were microscopically evaluated. While PBS-treated mice showed normal lung histology (Fig. 4A), mice that received 20 ng of SEB showed marked mononuclear cell infiltration in the parenchyma as well as around the bronchi (Fig. 4C). There was goblet cell hyperplasia in the bronchi suggestive of increased mucus production. Infiltration of eosinophils around the bronchi as well as the blood vessels was also evident. Mice that received 2,000 ng of SEB showed severe damage to the alveoli as well as to the bronchi, with loss of normal lung architecture (Fig. 4F).
Immunostaining with anti-MBP antibody showed only occasional eosinophils in PBS-treated mice (Fig. 4B). However, mice treated with 20 ng of SEB showed marked infiltration with eosinophils, especially around the airways and blood vessels (Fig. 4D). Extracellular MBP staining was also present, indicating degranulation of eosinophils (Fig. 4D). No MBP staining was observed with mice that had received 2,000 ng of SEB.
Systemic effects of superantigen delivered by the intranasal route. To study the systemic effect of SEB delivered intranasally, spleens and thymus glands were collected on day 8 and the distribution of CD4+ and CD8+ T cells expressing TCR V6 and V8 was analyzed by flow cytometry. As shown in Fig. 5A, there was a concentration-dependent increase in percentage of SEB-reactive TCR V8+ CD4+ T cells (P < 0.05 for all groups except PBS versus SEB 20-ng groups, one-way ANOVA) in the spleen. No differences in SEB nonreactive TCR V6+ CD4+ T cells were observed. There was a concentration-dependent reduction in TCR V8+ CD8+ T cells in SEB-treated mice compared to PBS-treated mice (P < 0.05 for all groups except PBS versus SEB 20-ng groups, one-way ANOVA). Repeated intranasal delivery of even small quantities of SEB also caused significant concentration-dependent apoptosis of CD4 CD8 double-positive thymocytes (Fig. 5B). Overall, these results suggest that SEB delivered into the nasal passage causes significant systemic T-cell activation at high but not at low concentrations of SEB.
Airway inflammation in HLA-DQ8 transgenic mice following intranasal superantigen delivery. We next confirmed the pathogenic effect of SEB on the respiratory system by using a second type of HLA class II (DQ8) transgenic mice. Since DQ molecules are less efficient in presenting SEB than are DR molecules (52), we tested only a higher concentration (1 μg) of SEB. As with HLA-DR3 transgenic mice, intranasal delivery of SEB was associated with recruitment of leukocytes into the BAL fluid (Fig. 6A to D) as well as eosinophilic airway inflammation. Flow cytometric analysis of BAL samples indicated the presence of more mononuclear cells (as represented by the gate) in mice that received SEB than in mice that received PBS. PBS-treated mice had only larger cells outside of the gate, with greater forward and side scatter, suggestive of alveolar macrophages (Fig. 6A). Further analysis of BAL fluid cells revealed the presence of CD4+ and CD8+ cells as well as B220+ cells (B cells) in mice that received SEB but not in PBS-treated mice (Fig. 6B and C). Giemsa-stained cytospin preparations revealed the presence of significantly higher numbers of lymphocytes (P < 0.05, unpaired Student's t test), eosinophils, and neutrophils in SEB-treated mice than in PBS-treated mice (Fig. 6D). As with HLA-DR3 mice, SEB-treated HLA-DQ8 mice had expansion of TCR V8+ but not TCR V6+ CD4+ T cells in the spleen. For unknown reasons, the CD8+ T cells were significantly reduced in SEB-treated mice (Fig. 6E).
We next wanted to study if an inflammatory airway response could be induced by a different superantigen, SPEA, which is presented efficiently by HLA-DQ molecules. We tested high (1 μg/application) as well as low (20 ng/application) concentrations of SPEA. Analysis of BAL fluid and spleens indicated that SPEA induced airway inflammation as well as systemic immune activation (Fig. 7; also data not shown). Histopathological evaluation of lungs from SPEA-challenged HLA-DQ8 or C57BL/6 mice indicated that SPEA elicited very little inflammatory change in C57BL/6 mice (Fig. 8A and B). Nonetheless, SPEA at the same dose induced marked pathology in HLA-DQ8 mice (Fig. 8C and D). SPEA at 2 ng/application also caused significant inflammatory changes only in HLA-DQ8 mice (Fig. 8E and F). Increasing the concentration of SPEA to 2 μg/application resulted in higher mortality in HLA-DQ8 mice (mortality, three of five mice, or 60%).
Increased eosinophil recruitment in STAT4-deficient DQ8 transgenic mice following intranasal superantigen challenge. To study the role of interleukin-12 (IL-12) cytokine signaling in SEB-induced airway inflammation, we challenged wild-type as well as STAT4-deficient (i.e., defective IL-12 signaling) HLA-DQ8 mice with a very low concentration of SEB (20 ng/application, administered three times). There was a significant increase in eosinophil recruitment into the airways in STAT4-deficient DQ8 mice compared to levels in STAT4-sufficient HLA-DQ8 mice (P < 0.05) (Fig. 9). There was no statistically significant difference in the numbers of other cell types in BAL fluid between these two groups. Lung sections from STAT4-deficient HLA-DQ8 mice showed a heightened inflammatory response (Fig. 10B). Immunostaining with anti-MBP antibodies revealed marked infiltration of eosinophils around the airways in STAT4-deficient HLA-DQ8 transgenic mice (Fig. 10E). This was accompanied by goblet cell hyperplasia (Fig. 10B). HLA-DQ8 mice that received a similar concentration of SEB intranasally showed only milder inflammation (Fig. 10C) and minimal eosinophil infiltration (Fig. 10F). Nonetheless, the inflammatory changes in SEB-challenged DQ8 mice were significantly greater than those in PBS-challenged HLA-DQ8 mice or PBS-challenged STAT4-deficient HLA-DQ8 mice (Fig. 10A). When the SEB concentration was increased to 1 μg/application, there was a dramatic increase in the eosinophilic airway inflammation and airway changes in STAT4-deficient HLA-DQ8 mice compared to levels in STAT4-sufficient HLA-DQ8 mice (Fig. 11). Thus, disrupting STAT4 signaling changed the nature of the inflammatory cells recruited to the airways by SEB, probably by shifting the cytokine pattern from the Th1 type to the Th2 type.
DISCUSSION
Superantigenic exotoxins are important virulence factors elaborated by S. aureus and S. pyogenes (51). Bacterial superantigens have special significance in human diseases because they bind to human MHC class II molecules with high affinity (51). While the effects of superantigens ingested through the oral route (e.g., food poisoning) or absorbed through the vaginal mucosa (i.e., menstrual toxic shock syndrome) have been studied (71), the effect of superantigen exposure through the airways has not been explored. This is important because S. aureus and S. pyogenes colonize the upper airways of humans (6, 13, 97) and act as a major source of infection especially of the respiratory tract (25, 91). About 2% of community-acquired pneumonias and up to 10% of hospital-acquired pneumonias are caused by S. aureus (62). In recent years, community-acquired lung infection due to methicillin-resistant S. aureus has emerged (13, 26, 109). While it has been shown that systemically delivered bacterial superantigens can be associated with pulmonary pathology (65), it has not been known whether superantigens gaining access through the intranasal route can cause airway and/or lung inflammation.
One published study did address the role of superantigens in airway inflammation by use of a murine model (39). However, this study used a conventional murine model which has inherent limitations in terms of studying the effects of bacterial superantigens. For example, while superantigen can activate murine T cells, about a 1,000-fold-higher concentration is required to achieve activation similar to that of human T cells (22), as a result of poor binding of bacterial superantigens to murine MHC class II molecules (24, 57, 82). Therefore, the superantigen-induced airway inflammation identified in this study is likely understated. Transgenic expression of human MHC (HLA) class II molecules in mice lacking endogenous class II molecules results in a dramatic increase in their T-cell responses to bacterial superantigens (16, 20, 73-76, 82, 94, 106, 110). Therefore, we used HLA class II transgenic mice to evaluate the ability of a staphylococcal superantigen and a streptococcal superantigen administered via the intranasal route to induce inflammation of the airways and lungs.
We confirmed earlier findings that HLA class II transgenic mice mount a robust systemic in vivo immune response to bacterial superantigen compared to the conventional mouse strain BALB/c (Fig. 1). We found that while intranasal delivery of SEB induces airway inflammation in both BALB/c and HLA-DR3 transgenic mice, the magnitude of the inflammation is significantly more pronounced in the HLA-DR3 mice, even with a lower concentration of SEB (Fig. 2). Therefore, we used HLA class II transgenic mice in subsequent experiments. The nature of the airway inflammation in HLA-DR3 transgenic mice is determined by the concentration of SEB delivered intranasally. A low concentration is associated with eosinophilic airway inflammation, whereas a higher concentration is associated with a neutrophilic inflammatory response (Fig. 3 and 4). In our study, higher concentrations of SEB were associated with elevated levels of IFN- in the BAL fluid (Fig. 3D) and higher mortality. It has previously been established that the concentration of superantigen determines the type of the immune response elicited, namely, Th1 versus Th2 (10). Low concentrations of superantigens polarize to a Th2-type response and high concentrations to a Th1-type response (9, 61, 81, 95). Accordingly, in our study low concentrations of SEB likely induced a Th2 response, resulting in eosinophilic airway inflammation and reversible AHR, whereas high concentrations of SEB likely induced a Th1 type of response, resulting in a neutrophilic response with irreversible airway changes and higher mortality. It has been shown with other models of inflammation that a Th1-type response is associated with neutrophil recruitment (23). The extent of lung pathology induced by superantigens administered intranasally in HLA-DR3 or DQ8 mice was more severe than that reported for a conventional mouse strain (39). We have performed additional experiments using HLA-DQ6 mice and found that SEB as well as SPEA can induce airway inflammation (G. Rajagopalan et al., unpublished data). All of these observations underscore our hypothesis that intranasal exposure to bacterial superantigen can induce dose-dependent airway/lung inflammation when HLA class II molecules are expressed.
Interleukin-12 is a strong inducer of Th1-type cytokines and a potent suppressor of Th2-type cytokines. Signaling through the IL-12 receptor is mediated through STAT4 (46), and as a result STAT4-deficient mice have severe defects in IL-12 receptor-mediated signaling. STAT4-deficient mice also have defective IFN- production. Due to profound defects in IL-12 and IFN-, T cells from STAT4-deficient mice preferentially produce more Th2-type cytokines following their activation (47). In this context, a corollary between atopic individuals and STAT4-deficient mice can be made, as STAT4-deficient mice, like atopic individuals, have a tendency towards increased production of Th2-type cytokines (1, 2, 11, 42, 47, 78, 107). The increased eosinophilic airway inflammation observed with STAT4-deficient mice suggests that SEB may induce more-severe eosinophilic airway inflammation in atopic individuals than in nonatopic individuals (Fig. 9 to 11). It should be noted that in the current study, mice were exposed to SEB for a very short period of time (i.e., 1 week). This period is probably insufficient for the induction of SEB-specific immunoglobulin E (80). Since isotype switching might take longer to occur (32), the superantigen-induced eosinophilic response observed in this study is probably immunoglobulin E independent (39). Since our study was conducted with humanized mice, our results lend support to the hypothesis that superantigens could play a definite role in asthma (4), as they do in related atopic disorders such as atopic dermatitis (55) and nasal polyps (5).
Mechanistically, superantigens likely cause airway inflammation through the same mechanisms by which they cause other diseases (71). There is abundant expression of MHC class II molecules within the airways and alveoli (18). There are professional as well as nonprofessional antigen-presenting cells present in airways and lungs (18, 19, 29, 53, 99). The airways are also lined with the bronchus-associated lymphatic tissues that contain antigen-presenting cells as well as T lymphocytes (98, 101). Therefore, when the superantigens reach the airways, they may directly bind to MHC class II molecules and vigorously activate both CD4+ and CD8+ T-cell subsets bearing certain TCR V families, culminating in airway inflammation. Superantigens can also directly induce mast cell degranulation and release of chemical mediators by binding to their MHC class II molecules and subsequent cross-linking (21, 72). By these mechanisms, airway exposure to superantigens may cause bronchoconstriction, mucus production, AHR, and airway inflammation.
The other interesting observation was that high concentrations of SEB delivered through the nasal mucosa caused systemic immune activation characterized by peripheral (i.e., splenic) T-cell expansion, thymic deletion, and even findings consistent with toxic shock. Absorption of SEB through mucous membranes other than that of the gut has not been demonstrated (63). Toxic shock syndrome toxin 1 is the only superantigen exotoxin known to be absorbed through nonenteric (i.e., vaginal) mucosa, with consequent menstrual toxic shock associated with the use of certain types of tampons (86). However, there are clinical observations suggesting that toxic shock syndrome may complicate respiratory tract infection (59, 93). To our understanding, ours is the first report to show that intranasal administration results in systemic immune activation and that, at higher concentrations, this mode of administration may lead to toxic shock and even death. This knowledge is important for understanding the potential effects of airway exposure to a high concentration of bacterial superantigens as a result of bioterrorism or accidental exposure (84, 92). It is well established that some bacterial superantigens interact more efficiently with certain HLA class II molecules. For example, SEB binds more efficiently to HLA-DR molecules (27, 38, 43, 87, 90) than to HLA-DQ, whereas SPEA is presented more efficiently by HLA-DQ than by HLA-DR (66, 94, 106). As a result, higher concentrations of SEB and SPEA elicited a robust immune response resulting in higher mortality in HLA-DR3 and HLA-DQ8 transgenic mice, respectively.
A recent study conducted using conventional mouse strains has proposed that mucosal exposure (i.e., through the nasal epithelium) to bacterial superantigens can induce tolerance against bacterial superantigens, which can protect against subsequent toxic shock induced by superantigens; translational use of this strategy with humans has been suggested (17). In light of our observations that intranasal exposure to superantigens can cause airway inflammation, such tolerance induction protocols should be cautiously approached.
Considering that nasopharyngeal colonization with S. aureus and S. pyogenes commonly occurs in humans and that these organisms are capable of elaborating superantigens (6, 35, 64), there is a distinct possibility that bacterial superantigens can reach the airways. Exposure to small amounts of bacterial superantigens, as might occur in the carrier state or during mild infection, could cause eosinophilic airway inflammation, especially in atopic individuals who have a tendency to mount a Th2-type immune response. Our hypothesis is strongly supported by a recent observation by Seiberling et al. (89) that at least one bacterial superantigen was detectable in nasal tissue from patients with chronic rhinosinusitis (a disease that has a strong association with nasal carriage of S. aureus [100]). They also found a strong positive correlation between eosinophil infiltration and superantigen detection. There also exists a strong positive correlation between rhinosinusitis and asthma (3). Since S. aureus and its superantigen exotoxins are associated with rhinosinusitis, superantigens may also play a role in the immunopathogenesis of asthma. Our hypothesis is further strengthened by a recent report by Okano et al., which states that intranasal administration of SEB facilitated development of allergic rhinitis in BALB/c mice when coadministered with Schistosoma mansoni egg antigen (67). Taken together, these reports provide substantial evidence that bacterial superantigens are produced in vivo. Our experimental model extends these observations and suggests that bacterial superantigens may induce airway inflammation (111). Human studies have also suggested a role for bacterial superantigens in the pathogenesis of asthma (3, 36). However, as asthma is a polygenic disease with strong environmental influence (8, 105), the low incidence of asthma compared to the carrier rate of S. aureus and S. pyogenes may be partly explained by the need for additional genetic and/or nongenetic risk factors. In conclusion, we support the hypothesis that superantigens may play a role in the etiopathogenesis of airway inflammation, including asthma. Bacterial superantigens, like SEB and SPEA, exposed through a nasal route can cause systemic immune activation and even death.
ACKNOWLEDGMENTS
We thank Julie Hanson and her crew for excellent mouse husbandry and Michelle Smart for characterizing the transgenic mice.
This study was supported by NIH grant AI14764. G.R. is the recipient of a Juvenile Diabetes Research Foundation fellowship.
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Division of Allergic Diseases Research
Division of Infectious Diseases, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, Minnesota 55905
ABSTRACT
Staphylococcus aureus is widely prevalent in the nasopharynges of healthy individuals (carriers) but can also cause serious infections. S. aureus can elaborate a variety of superantigen exotoxins in "carrier" or "pathogenic" states. Streptococcus pyogenes can also colonize the nasopharynx and elaborate superantigens. Unlike the acute effects of superantigen exotoxins absorbed through the gut or vaginal mucosa, little is known regarding the pathogenesis of superantigens entering through the intranasal route. In the current study, we evaluated the local and systemic effects of staphylococcal enterotoxin B (SEB) and streptococcal pyrogenic exotoxin A (SPEA) delivered through the intranasal route. Superantigens were administered intranasally on multiple occasions, and experimental animals were sacrificed on day 8 for experimental analyses. SEB-induced airway inflammation was more pronounced for HLA-DR3 transgenic mice than for BALB/c mice, consistent with bacterial superantigens binding more efficiently to human than murine major histocompatibility complex class II. The nature of the airway inflammation in HLA-DR3 mice was determined by the concentration of SEB applied intranasally. Low concentrations (20 ng) induced eosinophilic airway inflammation as well as eosinophil degranulation, whereas intranasal exposure to higher concentrations (2,000 ng) resulted in neutrophilic airway inflammation, permanent airway destruction, toxic shock, and mortality. SEB-induced eosinophilic inflammatory response was enhanced in signal transducer and activator of transcription (STAT)-4-deficient HLA-DQ8 transgenic mice with defective interleukin-12 signaling. Intranasal administration of SPEA induced airway inflammation and systemic immune activation in HLA-DQ8 transgenic mice. In conclusion, repeated chronic intranasal exposure to bacterial superantigens causes airway inflammation and systemic immune activation.
INTRODUCTION
Bacterial superantigens are a family of microbial polypeptide exotoxins capable of inducing strong proliferation of T lymphocytes even at extremely low concentrations (21, 63). They are the most potent T-cell mitogens known to humankind. Mechanistically, unlike conventional antigens which bind to the peptide-binding groove of major histocompatibility complex (MHC) class II molecules after undergoing a series of processing steps, superantigens bind directly to MHC class II molecules outside of the peptide-binding groove without undergoing processing. While exogenous antigens presented by MHC class II molecules activate CD4+ T cells (34), MHC class II-bound superantigens activate both CD4+ and CD8+ T cells. While conventional antigens activate specific T cells by interacting with both and chains of T-cell receptor (TCR) molecules (83), superantigens activate T cells by binding directly to the variable region of the TCR chain (and in rare cases TCR V families [69]), independent of their antigen specificities (56). Superantigens thus activate 30 to 50% of the total T-cell pool, whereas conventional antigens activate approximately 1 in 104 to 1 in 106 T cells (56). By virtue of their capacity to induce strong immune activation, superantigens can cause a variety of illnesses, ranging from acute food poisoning to toxic shock syndrome (21, 51), and have even been implicated in some autoimmune diseases (71, 108).
Bacterial superantigens are produced primarily by Staphylococcus aureus and Streptococcus pyogenes (71). S. aureus is ubiquitous in nature (7, 12, 58), and humans are natural carriers (primarily the nasal passage) (12, 68). While more than half of the pathogenic isolates produce one or more superantigen exotoxins (6, 28, 104), even strains isolated from asymptomatic carriers can produce superantigens (6, 28, 35, 49, 64, 103). The nasopharynx is also the site of colonization by group A, group C, and group G streptococci. Among these, S. pyogenes is considered the most pathogenic and elaborates a variety of superantigen exotoxins (77). Pathogenic S. pyogenes strains producing pyrogenic exotoxins have been isolated from asymptomatic schoolchildren living in areas with reported outbreaks of invasive streptococcal disease (15). While group C and group G streptococci are generally considered commensals, recent studies have demonstrated that they too can harbor genes encoding superantigen exotoxins (41, 45, 85). In addition to the presence of genes encoding bacterial superantigens in these isolates, recent studies have demonstrated the presence of transcripts encoding bacterial superantigens following experimental infection (102) and exotoxins themselves in patients with staphylococcal nasal carriage (89).
Given the presence of S. aureus and group A, group C, and group G streptococci in the human nasopharynx in either the carrier state or the disease state, it is probable that the respiratory tract is exposed to their products, including superantigens (3, 31, 40, 48, 70). It has been shown that staphylococcal protein A (30), cell wall peptidoglycan and lipoteichoic acid (54), and bacterial DNA containing unmethylated CpG motifs (88) can cause inflammation of the airways and lungs following direct exposure through the airway. However, the pathological effects of superantigen exotoxins produced by S. aureus and S. pyogenes on the respiratory system following direct exposure through the nasopharyngeal route have not been explored. As bacterial superantigens are potent T-cell mitogens, they have the potential to cause airway and lung inflammation through the same mechanisms by which they cause other diseases. While there are some indications that bacterial superantigens play a role in airway inflammation, including with asthma (4, 36, 37, 79) and chronic obstructive pulmonary disease (80), a definite role for bacterial superantigens in airway and/or lung inflammation has not yet been established using an appropriate animal model. Not only does this have immense clinical relevance, but the looming threat of the deployment of aerosolized superantigens as biological weapons necessitates elucidation of the effects of superantigen delivered directly to the nasal passage (33, 44, 60). In the current study, we evaluated the concentration-dependent effects of bacterial superantigen on the airways and lungs following intranasal exposure using a mouse model. Since bacterial superantigens bind poorly to murine MHC class II molecules (52), we used mice lacking endogenous class II but transgenically expressing high-affinity human HLA class II molecules (16, 73-76, 94, 110). Results of this study offer novel insight into the pathogenesis of airway inflammation.
MATERIALS AND METHODS
Mice. HLA-DR3 transgenic mice expressing functional HLA-DRA10101 and HLA-DRB10301 transgenes on the complete mouse MHC class II-deficient background (AEo) (14) have been previously described (74). Previously described HLA-DQ8 transgenic mice expressing HLA-DQA10301 and HLA-DQB10302 transgenes (74) were also studied. Signal transducer and activator of transcription (STAT)-4-deficient, HLA-DQ8 transgenic mice were generated by William Stohl (University of Southern California Keck School of Medicine, Los Angeles, CA). T-cell responses in these HLA transgenic mice are restricted only by the transgenic HLA class II molecules (96). BALB/c mice were from The Jackson Laboratory (Bar Harbor, ME). Mice were bred within the barrier facility of Mayo Clinic Immunogenetics Mouse Colony (Rochester, MN) and moved to a conventional facility after weaning. All experiments were approved by the Institutional Animal Care and Use Committee.
Superantigen and animal challenge. Staphylococcal enterotoxin B (SEB) and streptococcal pyrogenic exotoxin A (SPEA) (Toxin Laboratories, Sarasota, FL) were dissolved in sterile, endotoxin-free phosphate-buffered saline (PBS) and stored at –80°C. For intraperitoneal challenge, mice received the indicated concentrations of SEB in 200 μl of PBS. For intranasal challenge, mice were anesthetized with 2,2,2-tribromoethanol (Avertin; Aldrich Chemical, Milwaukee, WI), at a dose adjusted so that pedal reflexes were absent. Subsequently, mice were held in an upright position and 50 μl PBS alone or different concentrations of superantigens dissolved in 50 μl PBS were instilled into the nostrils. Mice were laid in a supine position until they recovered from anesthesia.
Measurement of AHR. Airway hyperreactivity (AHR) was assessed on the eighth day after the first intranasal SEB application by methacholine-induced airflow obstruction in conscious mice with a whole-body plethysmograph (Buxco Electronics, Troy, NY), using a previously described procedure (50). Pulmonary airflow obstruction was measured by enhanced pause (Penh) as follows: Penh = (Te/RT – 1) x (PEF/PIF), where Penh is enhanced pause (dimensionless), Te is expiratory time, RT is relaxation time, PEF is peak expiratory flow (ml/s), and PIF is peak inspiratory flow (ml/s). Penh, minute volume, tidal volume, and breathing frequency were obtained from chamber pressure, measured with a transducer connected to preamplifier modules, and analyzed by system software (all from Buxco Electronics). To measure methacholine responsiveness, mice were exposed to PBS for 2 min, followed by an incremental dosage (3 to 50 mg/ml) of aerosolized methacholine (freshly prepared in PBS); Penh was monitored for each dose of methacholine. Results for PBS and methacholine were expressed as percentage of baseline Penh values before PBS exposure.
Collection of BAL fluid. Immediately after AHR was measured, mice were injected intraperitoneally with a lethal dose of 2,2,2-tribromoethanol. The trachea was cannulated, the lungs were lavaged twice with 0.5 ml of PBS, and the fluids were pooled. After centrifugation, the supernatant was collected and stored at –80°C. The cells were resuspended and counted using a hemacytometer. Bronchoalveolar lavage (BAL) fluid cell differentials were determined by Wright-Giemsa staining; 200 cells were differentiated using conventional morphological criteria. Concentrations of gamma interferon (IFN-) in BAL fluid supernatants were measured using an enzyme-linked immunosorbent assay kit as directed by the manufacturer (R&D Systems, Minneapolis, MN).
Flow cytometry. Mononuclear cells from lymphoid organs and BAL fluid were stained with fluorescein isothiocyanate-conjugated anti-V6 or anti-V8, phycoerythrin-conjugated CD4, and peridinin chlorophyll protein-conjugated CD8. Alternatively, B cells and macrophages were analyzed using B220 and Mac-1 antibodies, respectively. All antibodies were from BD Pharmingen (San Diego, CA).
Histopathology and immunohistochemistry. After BAL fluid collection, the lungs were fixed in 10% formalin and embedded in paraffin. Six-millimeter sections were stained with hematoxylin and eosin for histopathological evaluation. For demonstrating eosinophils, processed formalin-fixed tissue sections were stained with rabbit anti-mouse major basic protein (MBP), as previously described (50).
Statistics. Statistical evaluation was carried out using InStat (version 3.0; GraphPad Software, Inc., San Diego, CA). Student's t test or analysis of variance (ANOVA) was performed, depending on the number of groups in each experiment.
RESULTS
Response to SEB in HLA-DR3 transgenic mice versus response in BALB/c mice. Intraperitoneal challenge with 10 μg of SEB resulted in a significantly higher (P < 0.05, unpaired Student's t test) expansion of CD4+ and CD8+ T cells bearing SEB-reactive TCR V8, but not the nonreactive TCR V6, in the spleens of HLA-DR3 transgenic mice than in spleens of BALB/c mice (Fig. 1A). Further, as depicted in Fig. 1B, the CD4 CD8 double-positive thymocytes in HLA-DR3 mice underwent a significant reduction compared to those in BALB/c mice (P < 0.001, unpaired Student's t test). Two injections of 10 μg SEB 48 h apart resulted in 100% mortality in HLA-DR3 mice (mortality, four of four mice), whereas all of the BALB/c mice tested survived this challenge (mortality, zero of four mice). These results confirm that HLA class II transgenic mice are better models for studying the immune response to superantigens than are conventional mouse strains.
Intranasal delivery of SEB results in inflammation of the airways and lungs in HLA-DR3 transgenic mice. To compare the direct effects of SEB on the airways of HLA-DR3 and BALB/c mice, we challenged these two strains with the indicated concentrations of SEB by the intranasal route (Fig. 2). Intranasal delivery of SEB resulted in a significantly higher accumulation of macrophages, eosinophils, neutrophils, and lymphocytes in BAL fluid of HLA-DR3 mice than in BAL fluid of BALB/c mice (P < 0.05, unpaired Student's t test), even though the latter were challenged with 2.5 times the dose used for the former. Only alveolar macrophages were detected in BAL fluid of PBS-challenged mice from either group. Since SEB elicited more-severe airway inflammation in HLA-DR3 mice than in BALB/c mice, the former were used in subsequent studies.
Concentration-dependent variations in airway responses to SEB in HLA-DR3 transgenic mice. We evaluated the effect of SEB concentration (20, 200, or 2,000 ng) on airway inflammation by challenging HLA-DR3 mice with increasing concentrations of SEB and measuring airway responses on the eighth day. Mice administered 20 ng of SEB did not show any visible signs of distress and appeared healthy throughout the study. However, mice administered 2,000 ng of SEB exhibited clear signs of distress, including ruffled fur, labored breathing, listlessness, and weight loss. While the body weights of the mice in all groups were comparable on day 0, the body weights of the PBS group was 25.25 ± 1.70 g (n = 9) on day 8, while that of the SEB 2,000-ng group was 16.6 ± 1.7 g (n = 4) (P = 0.0002, unpaired Student's t test).
While no mortality was noted after the first dose of intranasal SEB delivery for any of the groups studied, high mortality was observed after the second or third dose of intranasal SEB delivery for the 200-ng (mortality, 4/10 mice) and 2,000-ng (mortality, 6/10 mice) groups. Mortality was not observed during or immediately after administration of SEB but rather occurred during the ensuing 24 to 48 h. Thus, airway exposure to low concentrations of SEB did not cause any apparent clinical signs, while higher concentrations of SEB caused signs consistent with toxic shock and were associated with mortality.
We evaluated AHR in mice that had been administered SEB or PBS intranasally by measuring the methacholine-induced airway changes at the termination of the experiment (i.e., on day 8). Mice that received PBS and 20 ng/dose of SEB had comparable baseline AHRs as measured by Penh. However, airway resistance in the SEB 20-ng group showed definite changes in response to increasing concentrations of inhaled methacholine compared to airway resistance in the PBS-treated group (P = 0.25, 0.045, 0.05, and 0.313 at 3, 6, 12, and 25 mg of methacholine, respectively; unpaired Student's t test) (Fig. 3A). On the other hand, mice that received 200 and 2,000 ng of SEB had significantly higher baseline AHRs than mice that received intranasal PBS (P < 0.05, ANOVA). AHR changed little following methacholine challenge in these groups. This indicated that while a low concentration of SEB (20 ng) was associated with reversible AHR, higher concentrations of SEB (200 and 2,000 ng) were associated with permanent airway changes and showed little methacholine-induced change (Fig. 3A), suggesting a concentration-dependent effect of SEB on certain airway responses.
We next studied the effect of variation in SEB concentration on the types of leukocytes recruited to the airways by analyzing the BAL fluid (Fig. 3B). Lower concentrations of SEB recruited more eosinophils into BAL fluid than did higher concentrations of SEB (P < 0.01 for PBS versus SEB 20-ng groups; P < 0.05 for PBS versus SEB 200-ng groups as well as SEB 20-ng versus 2,000-ng groups; one-way ANOVA). Higher concentrations of SEB were associated with more neutrophil recruitment into BAL fluid than were lower concentrations (P < 0.05 for all SEB groups compared to the PBS group; P < 0.001 for SEB 2,000-ng versus 20-ng or 200-ng groups; one-way ANOVA). The eosinophil-to-neutrophil ratios were 1.6, 0.75, and 0.03 for 20, 200, and 2,000 ng/dose of intranasal SEB, respectively. Macrophages were more abundant at lower SEB concentrations (P < 0.01 for PBS versus SEB 20-ng groups as well as PBS versus SEB 200-ng groups; P < 0.05 for SEB 20-ng versus SEB 2,000-ng groups; one-way ANOVA). An inverse concentration effect was also seen for lymphocyte recruitment. Lymphocytes were more abundant at lower SEB concentrations (Fig. 3B and C) (P < 0.01 for PBS versus SEB 20-ng groups; P < 0.05 for PBS versus SEB 200-ng groups as well as SEB 20-ng versus SEB 2,000-ng groups; one-way ANOVA). Together, these results suggest that bacterial superantigens are capable of inducing concentration-dependent AHR and that the characteristics of inflammatory-cell recruitment vary in a SEB concentration-dependent manner. We also quantified the levels of IFN- in BAL fluid from superantigen- or PBS-challenged mice (Fig. 3D). IFN- levels were significantly higher in SEB 200-ng and SEB 2,000-ng groups than in either PBS or SEB 20-ng groups (P < 0.05).
To further evaluate the pathological changes in the lungs following SEB challenge, paraffin-fixed lung sections were microscopically evaluated. While PBS-treated mice showed normal lung histology (Fig. 4A), mice that received 20 ng of SEB showed marked mononuclear cell infiltration in the parenchyma as well as around the bronchi (Fig. 4C). There was goblet cell hyperplasia in the bronchi suggestive of increased mucus production. Infiltration of eosinophils around the bronchi as well as the blood vessels was also evident. Mice that received 2,000 ng of SEB showed severe damage to the alveoli as well as to the bronchi, with loss of normal lung architecture (Fig. 4F).
Immunostaining with anti-MBP antibody showed only occasional eosinophils in PBS-treated mice (Fig. 4B). However, mice treated with 20 ng of SEB showed marked infiltration with eosinophils, especially around the airways and blood vessels (Fig. 4D). Extracellular MBP staining was also present, indicating degranulation of eosinophils (Fig. 4D). No MBP staining was observed with mice that had received 2,000 ng of SEB.
Systemic effects of superantigen delivered by the intranasal route. To study the systemic effect of SEB delivered intranasally, spleens and thymus glands were collected on day 8 and the distribution of CD4+ and CD8+ T cells expressing TCR V6 and V8 was analyzed by flow cytometry. As shown in Fig. 5A, there was a concentration-dependent increase in percentage of SEB-reactive TCR V8+ CD4+ T cells (P < 0.05 for all groups except PBS versus SEB 20-ng groups, one-way ANOVA) in the spleen. No differences in SEB nonreactive TCR V6+ CD4+ T cells were observed. There was a concentration-dependent reduction in TCR V8+ CD8+ T cells in SEB-treated mice compared to PBS-treated mice (P < 0.05 for all groups except PBS versus SEB 20-ng groups, one-way ANOVA). Repeated intranasal delivery of even small quantities of SEB also caused significant concentration-dependent apoptosis of CD4 CD8 double-positive thymocytes (Fig. 5B). Overall, these results suggest that SEB delivered into the nasal passage causes significant systemic T-cell activation at high but not at low concentrations of SEB.
Airway inflammation in HLA-DQ8 transgenic mice following intranasal superantigen delivery. We next confirmed the pathogenic effect of SEB on the respiratory system by using a second type of HLA class II (DQ8) transgenic mice. Since DQ molecules are less efficient in presenting SEB than are DR molecules (52), we tested only a higher concentration (1 μg) of SEB. As with HLA-DR3 transgenic mice, intranasal delivery of SEB was associated with recruitment of leukocytes into the BAL fluid (Fig. 6A to D) as well as eosinophilic airway inflammation. Flow cytometric analysis of BAL samples indicated the presence of more mononuclear cells (as represented by the gate) in mice that received SEB than in mice that received PBS. PBS-treated mice had only larger cells outside of the gate, with greater forward and side scatter, suggestive of alveolar macrophages (Fig. 6A). Further analysis of BAL fluid cells revealed the presence of CD4+ and CD8+ cells as well as B220+ cells (B cells) in mice that received SEB but not in PBS-treated mice (Fig. 6B and C). Giemsa-stained cytospin preparations revealed the presence of significantly higher numbers of lymphocytes (P < 0.05, unpaired Student's t test), eosinophils, and neutrophils in SEB-treated mice than in PBS-treated mice (Fig. 6D). As with HLA-DR3 mice, SEB-treated HLA-DQ8 mice had expansion of TCR V8+ but not TCR V6+ CD4+ T cells in the spleen. For unknown reasons, the CD8+ T cells were significantly reduced in SEB-treated mice (Fig. 6E).
We next wanted to study if an inflammatory airway response could be induced by a different superantigen, SPEA, which is presented efficiently by HLA-DQ molecules. We tested high (1 μg/application) as well as low (20 ng/application) concentrations of SPEA. Analysis of BAL fluid and spleens indicated that SPEA induced airway inflammation as well as systemic immune activation (Fig. 7; also data not shown). Histopathological evaluation of lungs from SPEA-challenged HLA-DQ8 or C57BL/6 mice indicated that SPEA elicited very little inflammatory change in C57BL/6 mice (Fig. 8A and B). Nonetheless, SPEA at the same dose induced marked pathology in HLA-DQ8 mice (Fig. 8C and D). SPEA at 2 ng/application also caused significant inflammatory changes only in HLA-DQ8 mice (Fig. 8E and F). Increasing the concentration of SPEA to 2 μg/application resulted in higher mortality in HLA-DQ8 mice (mortality, three of five mice, or 60%).
Increased eosinophil recruitment in STAT4-deficient DQ8 transgenic mice following intranasal superantigen challenge. To study the role of interleukin-12 (IL-12) cytokine signaling in SEB-induced airway inflammation, we challenged wild-type as well as STAT4-deficient (i.e., defective IL-12 signaling) HLA-DQ8 mice with a very low concentration of SEB (20 ng/application, administered three times). There was a significant increase in eosinophil recruitment into the airways in STAT4-deficient DQ8 mice compared to levels in STAT4-sufficient HLA-DQ8 mice (P < 0.05) (Fig. 9). There was no statistically significant difference in the numbers of other cell types in BAL fluid between these two groups. Lung sections from STAT4-deficient HLA-DQ8 mice showed a heightened inflammatory response (Fig. 10B). Immunostaining with anti-MBP antibodies revealed marked infiltration of eosinophils around the airways in STAT4-deficient HLA-DQ8 transgenic mice (Fig. 10E). This was accompanied by goblet cell hyperplasia (Fig. 10B). HLA-DQ8 mice that received a similar concentration of SEB intranasally showed only milder inflammation (Fig. 10C) and minimal eosinophil infiltration (Fig. 10F). Nonetheless, the inflammatory changes in SEB-challenged DQ8 mice were significantly greater than those in PBS-challenged HLA-DQ8 mice or PBS-challenged STAT4-deficient HLA-DQ8 mice (Fig. 10A). When the SEB concentration was increased to 1 μg/application, there was a dramatic increase in the eosinophilic airway inflammation and airway changes in STAT4-deficient HLA-DQ8 mice compared to levels in STAT4-sufficient HLA-DQ8 mice (Fig. 11). Thus, disrupting STAT4 signaling changed the nature of the inflammatory cells recruited to the airways by SEB, probably by shifting the cytokine pattern from the Th1 type to the Th2 type.
DISCUSSION
Superantigenic exotoxins are important virulence factors elaborated by S. aureus and S. pyogenes (51). Bacterial superantigens have special significance in human diseases because they bind to human MHC class II molecules with high affinity (51). While the effects of superantigens ingested through the oral route (e.g., food poisoning) or absorbed through the vaginal mucosa (i.e., menstrual toxic shock syndrome) have been studied (71), the effect of superantigen exposure through the airways has not been explored. This is important because S. aureus and S. pyogenes colonize the upper airways of humans (6, 13, 97) and act as a major source of infection especially of the respiratory tract (25, 91). About 2% of community-acquired pneumonias and up to 10% of hospital-acquired pneumonias are caused by S. aureus (62). In recent years, community-acquired lung infection due to methicillin-resistant S. aureus has emerged (13, 26, 109). While it has been shown that systemically delivered bacterial superantigens can be associated with pulmonary pathology (65), it has not been known whether superantigens gaining access through the intranasal route can cause airway and/or lung inflammation.
One published study did address the role of superantigens in airway inflammation by use of a murine model (39). However, this study used a conventional murine model which has inherent limitations in terms of studying the effects of bacterial superantigens. For example, while superantigen can activate murine T cells, about a 1,000-fold-higher concentration is required to achieve activation similar to that of human T cells (22), as a result of poor binding of bacterial superantigens to murine MHC class II molecules (24, 57, 82). Therefore, the superantigen-induced airway inflammation identified in this study is likely understated. Transgenic expression of human MHC (HLA) class II molecules in mice lacking endogenous class II molecules results in a dramatic increase in their T-cell responses to bacterial superantigens (16, 20, 73-76, 82, 94, 106, 110). Therefore, we used HLA class II transgenic mice to evaluate the ability of a staphylococcal superantigen and a streptococcal superantigen administered via the intranasal route to induce inflammation of the airways and lungs.
We confirmed earlier findings that HLA class II transgenic mice mount a robust systemic in vivo immune response to bacterial superantigen compared to the conventional mouse strain BALB/c (Fig. 1). We found that while intranasal delivery of SEB induces airway inflammation in both BALB/c and HLA-DR3 transgenic mice, the magnitude of the inflammation is significantly more pronounced in the HLA-DR3 mice, even with a lower concentration of SEB (Fig. 2). Therefore, we used HLA class II transgenic mice in subsequent experiments. The nature of the airway inflammation in HLA-DR3 transgenic mice is determined by the concentration of SEB delivered intranasally. A low concentration is associated with eosinophilic airway inflammation, whereas a higher concentration is associated with a neutrophilic inflammatory response (Fig. 3 and 4). In our study, higher concentrations of SEB were associated with elevated levels of IFN- in the BAL fluid (Fig. 3D) and higher mortality. It has previously been established that the concentration of superantigen determines the type of the immune response elicited, namely, Th1 versus Th2 (10). Low concentrations of superantigens polarize to a Th2-type response and high concentrations to a Th1-type response (9, 61, 81, 95). Accordingly, in our study low concentrations of SEB likely induced a Th2 response, resulting in eosinophilic airway inflammation and reversible AHR, whereas high concentrations of SEB likely induced a Th1 type of response, resulting in a neutrophilic response with irreversible airway changes and higher mortality. It has been shown with other models of inflammation that a Th1-type response is associated with neutrophil recruitment (23). The extent of lung pathology induced by superantigens administered intranasally in HLA-DR3 or DQ8 mice was more severe than that reported for a conventional mouse strain (39). We have performed additional experiments using HLA-DQ6 mice and found that SEB as well as SPEA can induce airway inflammation (G. Rajagopalan et al., unpublished data). All of these observations underscore our hypothesis that intranasal exposure to bacterial superantigen can induce dose-dependent airway/lung inflammation when HLA class II molecules are expressed.
Interleukin-12 is a strong inducer of Th1-type cytokines and a potent suppressor of Th2-type cytokines. Signaling through the IL-12 receptor is mediated through STAT4 (46), and as a result STAT4-deficient mice have severe defects in IL-12 receptor-mediated signaling. STAT4-deficient mice also have defective IFN- production. Due to profound defects in IL-12 and IFN-, T cells from STAT4-deficient mice preferentially produce more Th2-type cytokines following their activation (47). In this context, a corollary between atopic individuals and STAT4-deficient mice can be made, as STAT4-deficient mice, like atopic individuals, have a tendency towards increased production of Th2-type cytokines (1, 2, 11, 42, 47, 78, 107). The increased eosinophilic airway inflammation observed with STAT4-deficient mice suggests that SEB may induce more-severe eosinophilic airway inflammation in atopic individuals than in nonatopic individuals (Fig. 9 to 11). It should be noted that in the current study, mice were exposed to SEB for a very short period of time (i.e., 1 week). This period is probably insufficient for the induction of SEB-specific immunoglobulin E (80). Since isotype switching might take longer to occur (32), the superantigen-induced eosinophilic response observed in this study is probably immunoglobulin E independent (39). Since our study was conducted with humanized mice, our results lend support to the hypothesis that superantigens could play a definite role in asthma (4), as they do in related atopic disorders such as atopic dermatitis (55) and nasal polyps (5).
Mechanistically, superantigens likely cause airway inflammation through the same mechanisms by which they cause other diseases (71). There is abundant expression of MHC class II molecules within the airways and alveoli (18). There are professional as well as nonprofessional antigen-presenting cells present in airways and lungs (18, 19, 29, 53, 99). The airways are also lined with the bronchus-associated lymphatic tissues that contain antigen-presenting cells as well as T lymphocytes (98, 101). Therefore, when the superantigens reach the airways, they may directly bind to MHC class II molecules and vigorously activate both CD4+ and CD8+ T-cell subsets bearing certain TCR V families, culminating in airway inflammation. Superantigens can also directly induce mast cell degranulation and release of chemical mediators by binding to their MHC class II molecules and subsequent cross-linking (21, 72). By these mechanisms, airway exposure to superantigens may cause bronchoconstriction, mucus production, AHR, and airway inflammation.
The other interesting observation was that high concentrations of SEB delivered through the nasal mucosa caused systemic immune activation characterized by peripheral (i.e., splenic) T-cell expansion, thymic deletion, and even findings consistent with toxic shock. Absorption of SEB through mucous membranes other than that of the gut has not been demonstrated (63). Toxic shock syndrome toxin 1 is the only superantigen exotoxin known to be absorbed through nonenteric (i.e., vaginal) mucosa, with consequent menstrual toxic shock associated with the use of certain types of tampons (86). However, there are clinical observations suggesting that toxic shock syndrome may complicate respiratory tract infection (59, 93). To our understanding, ours is the first report to show that intranasal administration results in systemic immune activation and that, at higher concentrations, this mode of administration may lead to toxic shock and even death. This knowledge is important for understanding the potential effects of airway exposure to a high concentration of bacterial superantigens as a result of bioterrorism or accidental exposure (84, 92). It is well established that some bacterial superantigens interact more efficiently with certain HLA class II molecules. For example, SEB binds more efficiently to HLA-DR molecules (27, 38, 43, 87, 90) than to HLA-DQ, whereas SPEA is presented more efficiently by HLA-DQ than by HLA-DR (66, 94, 106). As a result, higher concentrations of SEB and SPEA elicited a robust immune response resulting in higher mortality in HLA-DR3 and HLA-DQ8 transgenic mice, respectively.
A recent study conducted using conventional mouse strains has proposed that mucosal exposure (i.e., through the nasal epithelium) to bacterial superantigens can induce tolerance against bacterial superantigens, which can protect against subsequent toxic shock induced by superantigens; translational use of this strategy with humans has been suggested (17). In light of our observations that intranasal exposure to superantigens can cause airway inflammation, such tolerance induction protocols should be cautiously approached.
Considering that nasopharyngeal colonization with S. aureus and S. pyogenes commonly occurs in humans and that these organisms are capable of elaborating superantigens (6, 35, 64), there is a distinct possibility that bacterial superantigens can reach the airways. Exposure to small amounts of bacterial superantigens, as might occur in the carrier state or during mild infection, could cause eosinophilic airway inflammation, especially in atopic individuals who have a tendency to mount a Th2-type immune response. Our hypothesis is strongly supported by a recent observation by Seiberling et al. (89) that at least one bacterial superantigen was detectable in nasal tissue from patients with chronic rhinosinusitis (a disease that has a strong association with nasal carriage of S. aureus [100]). They also found a strong positive correlation between eosinophil infiltration and superantigen detection. There also exists a strong positive correlation between rhinosinusitis and asthma (3). Since S. aureus and its superantigen exotoxins are associated with rhinosinusitis, superantigens may also play a role in the immunopathogenesis of asthma. Our hypothesis is further strengthened by a recent report by Okano et al., which states that intranasal administration of SEB facilitated development of allergic rhinitis in BALB/c mice when coadministered with Schistosoma mansoni egg antigen (67). Taken together, these reports provide substantial evidence that bacterial superantigens are produced in vivo. Our experimental model extends these observations and suggests that bacterial superantigens may induce airway inflammation (111). Human studies have also suggested a role for bacterial superantigens in the pathogenesis of asthma (3, 36). However, as asthma is a polygenic disease with strong environmental influence (8, 105), the low incidence of asthma compared to the carrier rate of S. aureus and S. pyogenes may be partly explained by the need for additional genetic and/or nongenetic risk factors. In conclusion, we support the hypothesis that superantigens may play a role in the etiopathogenesis of airway inflammation, including asthma. Bacterial superantigens, like SEB and SPEA, exposed through a nasal route can cause systemic immune activation and even death.
ACKNOWLEDGMENTS
We thank Julie Hanson and her crew for excellent mouse husbandry and Michelle Smart for characterizing the transgenic mice.
This study was supported by NIH grant AI14764. G.R. is the recipient of a Juvenile Diabetes Research Foundation fellowship.
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