The isolation of the nuclear lamina
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《细胞学杂志》
Aaronson and Blobel (1974) used detergent to peel away the membranes from isolated nuclei. The husks that remained held their shape. This sturdy layer, they proposed, was the lamina, and the results suggested two of its functions—bracing the nucleus and cradling the nuclear pores. Two follow-up studies (Aaronson and Blobel, 1975; Dwyer and Blobel, 1976) provided more evidence that the layer they had identified enclosed the nucleus and wasn't just part of the membrane.
Then Larry Gerace (Scripps Research Institute, La Jolla, CA), Blobel's first Ph.D. student, picked up the analysis. He wanted to definitively describe the lamina in part because of what he viewed as the erroneous conclusions of Berezney and Coffey (1977). They had proposed that proteins not only formed the lamina but also a "nuclear matrix" that extended throughout the nucleus and intermingled with the DNA. "Our localization was a riposte to their conclusions," Gerace says. He characterized three lamina proteins and created antibodies against them. Immunoperoxidase staining showed that the antibodies strongly labeled the rim of the nucleus; they didn't recognize anything in the interior (Gerace et al., 1978). Rather than a mesh that permeated the nucleus, the lamina was a protein polymer that hugged the nuclear membrane, the researchers concluded—and subsequent work has backed them up.
An immunoperoxidase stain that tags one lamina protein doesn't penetrate the nucleus ("La" indicates the lamina; arrows mark nuclear pores).
BLOBEL
The proteins Gerace identified turned out to be lamins A, B1, and C, three of the four major components that interweave to form the lamina. "We felt we had made a conclusive argument that lamins are primarily at the nuclear envelope, and the data have held up," says Gerace. Blobel describes this series of studies as one of the first examples of molecular cell biology. Instead of being content to identify new cellular structures, researchers were now breaking down these discoveries into their molecular components to elucidate their workings. ML
Aaronson, R.P., and G. Blobel. 1974. J. Cell Biol. 62:746–754.
Aaronson, R.P., and G. Blobel. 1975. Proc. Natl. Acad. Sci. USA. 72:1007–1011.
Berezney, R., and D.S. Coffey. 1977. J. Cell Biol. 73:616–637.
Dwyer, N., and G. Blobel. 1976. J. Cell Biol. 70:581–591.
Gerace, L., et al. 1978. J. Cell Biol. 79:546–566.(The number of monikers early cell biolog)
Then Larry Gerace (Scripps Research Institute, La Jolla, CA), Blobel's first Ph.D. student, picked up the analysis. He wanted to definitively describe the lamina in part because of what he viewed as the erroneous conclusions of Berezney and Coffey (1977). They had proposed that proteins not only formed the lamina but also a "nuclear matrix" that extended throughout the nucleus and intermingled with the DNA. "Our localization was a riposte to their conclusions," Gerace says. He characterized three lamina proteins and created antibodies against them. Immunoperoxidase staining showed that the antibodies strongly labeled the rim of the nucleus; they didn't recognize anything in the interior (Gerace et al., 1978). Rather than a mesh that permeated the nucleus, the lamina was a protein polymer that hugged the nuclear membrane, the researchers concluded—and subsequent work has backed them up.
An immunoperoxidase stain that tags one lamina protein doesn't penetrate the nucleus ("La" indicates the lamina; arrows mark nuclear pores).
BLOBEL
The proteins Gerace identified turned out to be lamins A, B1, and C, three of the four major components that interweave to form the lamina. "We felt we had made a conclusive argument that lamins are primarily at the nuclear envelope, and the data have held up," says Gerace. Blobel describes this series of studies as one of the first examples of molecular cell biology. Instead of being content to identify new cellular structures, researchers were now breaking down these discoveries into their molecular components to elucidate their workings. ML
Aaronson, R.P., and G. Blobel. 1974. J. Cell Biol. 62:746–754.
Aaronson, R.P., and G. Blobel. 1975. Proc. Natl. Acad. Sci. USA. 72:1007–1011.
Berezney, R., and D.S. Coffey. 1977. J. Cell Biol. 73:616–637.
Dwyer, N., and G. Blobel. 1976. J. Cell Biol. 70:581–591.
Gerace, L., et al. 1978. J. Cell Biol. 79:546–566.(The number of monikers early cell biolog)