An Aptamer That Neutralizes R5 Strains of Human Im
http://www.100md.com
病菌学杂志 2005年第21期
Laboratory of Molecular Biophysics, Department of Biochemistry
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892
ABSTRACT
We recently described the isolation and structural characterization of 2'-fluoropyrimidine-substituted RNA aptamers that bind to gp120 of R5 strains of human immunodeficiency virus type 1 and thereby potently neutralize the infectivity of phylogenetically diverse R5 strains. Here we investigate the physical basis of their antiviral action. We show that both N-linked oligosaccharides and the variable loops V1/V2 and V3 are not required for binding of one aptamer, B40, to gp120. Using surface plasmon resonance binding analyses, we show that the aptamer binds to the CCR5-binding site on gp120 in a relatively CD4-independent manner, providing a mechanistic explanation for its neutralizing potency.
TEXT
The entry of human immunodeficiency virus type 1 (HIV-1) into target cells depends on the sequential interaction of the surface envelope glycoprotein, gp120, with the primary receptor, CD4, and the coreceptor, CCR5 (1, 5, 7, 9, 15, 29), and is an attractive target for antiviral intervention. However, gp120 is a challenging target because of the conformational flexibility and sequence variability it has evolved to counter host antibody responses (21, 37). The CD4-binding site is recessed and partially obscured from antibody access by the V1/V2 hypervariable loops and associated carbohydrate structures (37). Considerable conformational flexibility of the CD4-unbound state of gp120 further masks the conserved receptor binding site and hinders antibody access (19, 22, 27). The coreceptor binding site on gp120, which is thought to be composed of highly conserved elements of the ?19 strand and parts of the V3 hypervariable loop (28, 29, 39), is also protected by the V1/V2 loops from antibody access in the CD4-unbound state (4, 34, 36). Binding of CD4 induces conformational changes in gp120 that include the displacement of the V1/V2 stem-loop structure, thereby revealing a functional coreceptor binding site (26, 31, 38). Recent studies have also shown that this CD4-induced (CD4i) epitope on gp120 is protected from antibodies by steric hindrance and that the size of the neutralizing agent directed towards this region inversely correlates with its ability to neutralize (22). Mutational variation and extensive glycosylation of gp120 further add to the ineffectiveness of the humoral immune response.
In response to these challenges, one rational antiviral approach is to generate small ligands directed to the conserved core regions of gp120 that are critical for viral entry. We hypothesized that aptamers (11, 35), by virtue of their small size compared to antibodies, could access the conserved pockets in gp120, thereby blocking infection. Recently, we isolated and structurally characterized 2'F RNA aptamers that bind gp120 of HIV-1 R5 strain Ba-L and potently neutralize a broad range of HIV-1 primary isolates (8, 18). In order to test whether, as we had hypothesized, the mechanism of neutralization by aptamers was via ligation of critical receptor-binding regions, we mapped the binding site of one such aptamer (B40) using competition with gp120's natural ligands by deletion analysis and by deglycosylation.
The binding of aptamer to gp120 is independent of N-linked glycosylation. Recombinant monomeric Ba-L gp120 was expressed in insect cells using a baculovirus expression system, as previously described (18, 24), and deglycosylated using endo-?-N-acetylglucosaminidase H (endo H) under native conditions as previously described (20). When analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, endo H treatment of gp120 was found to have resulted in the complete disappearance of the fully glycosylated material (apparent mass 92 kDa) with the majority now migrating with an apparent mass of 63 kDa (Fig. 1A). Equimolar amounts of both the glycosylated and deglycosylated gp120 were then analyzed for ability to bind aptamer by BIAcore surface plasmon resonance analysis as previously described (8). The aptamer bound to both glycosylated and deglycosylated gp120 in a similar manner (Fig. 1B), indicating that the aptamer binding site on gp120 is not dependent on the N-linked glycans on the glycoprotein.
In contrast, monoclonal antibody (MAb) 2G12, which binds to a glycan-dependent epitope (30, 33), bound to the glycosylated but not the deglycosylated gp120, as expected (Fig. 1C). Soluble CD4 (sCD4) bound better to the glycosylated gp120 than to the deglycosylated gp120 (Fig. 1D), consistent with previous reports (16, 23, 25).
Role of V1/V2 and V3 loops in aptamer binding. Our results, above, show that one can remove the majority of N-linked sugars from gp120 without affecting the binding site for aptamer B40 but do not exclude the possibility that a specific oligosaccharide, refractory to deglycosylation, plays an important role. The most likely candidates for such an oligosaccharide lie within the hypervariable loops V1/V2 and V3, as they would be close to sites already known to be important for interaction of gp120 with receptor and coreceptor molecules (21, 28, 29, 37). In order to simultaneously investigate this possibility of a residual oligosaccharide and the potential that aptamers bound directly to these loop sequences, we used protein constructs, derived from the R5 strain Yu2, in which one or both loops are deleted and which lack complex sugars (17, 38). The results of a BIAcore analysis (Fig. 2C) show that aptamer B40 binds to the loop-deleted core of gp120, and therefore its binding site does not lie within the V1/V2 or V3 loops nor the oligosaccharides associated with them. It appears that the aptamer binding site is somewhat obscured in the core as well as the core plus V3 constructs of gp120 and becomes fully exposed only following the binding of CD4 (Fig. 2B and 2C). This is in marked contrast to the ability of aptamer B40 to bind full-length gp120 in a largely CD4-independent manner (Fig. 2A and Table 1). We tentatively conclude from this that the aptamer binding site, though located in core regions of gp120, is maintained in its aptamer-competent state by multiple interloop interactions and further that, when deletion of the loop causes the partial collapse of the aptamer binding pocket, the binding of CD4 allosterically reopens the pocket.
The aptamer binding site overlaps with the CCR5-binding site on gp120. We have previously shown that aptamer B4, which shares critical structural motifs with aptamer B40, competes for binding to gp120 with the CD4i MAb 17b (8, 18). Taken together with the CD4 dependence of efficient binding of aptamer B40 to the core gp120 construct described above, this pointed strongly to the possibility that the aptamer binding site was close to that of CCR5. To test this, we carried out a BIAcore surface plasmon resonance competition binding analysis using the 22-mer tyrosine-sulfated peptide (S-peptide) corresponding to the N terminus of CCR5 that acts as an essential component of the coreceptor for HIV-1 on the host cell (2, 6, 10, 12-14), as well as an unsulfated version of the same peptide (C-peptide) and sCD4, as alternate immobilized binding partners for gp120 (Fig. 3). As controls, RANTES, a physiological ligand for both CCR3 and CCR5 (3), and the anti-CCR5 MAb 3A9, but not monocyte chemoattractant protein 1, a ligand for CCR2 and CCR4, bound to the CCR5 peptides, as expected (data not shown).
Monomeric Ba-L gp120 bound well to immobilized CD4 but weakly to the S-peptide and negligibly to the C-peptide (Fig. 3A). However, preincubating gp120 with CD4 increased binding to both CCR5 peptides by more than 2.5-fold (Fig. 3B), confirming the CD4-induced binding property of the peptides to gp120 (14). Moreover, the approximately threefold difference in binding of both gp120 and gp120-sCD4 complex to the S-peptide versus C-peptide confirm that the tyrosine sulfate moieties contribute substantially to the association of the gp120-sCD4 complexes, as previously reported (13). As expected, the gp120-sCD4 complex did not bind to immobilized CD4 (Fig. 3B). When the gp120-17b MAb complex was injected, it bound only to the immobilized CD4 and not to the CCR5 peptides (data not shown), also as expected. The gp120-aptamer B40 complex also bound to the immobilized CD4 but not to the CCR5 peptides (Fig. 3C). The ternary complexes, gp120-sCD4-17 MAb (data not shown) and gp120-sCD4-aptamer B40 (Fig. 3D), did not bind to either the immobilized CCR5 peptides or human CD4.
Taken together, these results show that the aptamer competes with the N-terminal ectodomain of CCR5 for binding to gp120 and hence the aptamer binding site (aptatope) must overlap with the basic, conserved, coreceptor-binding region on gp120. This is consistent with the inability of aptamer B40 to bind the gp120 of the X4 strain, HXB2 (data not shown) in contrast to aptamers raised against X4 strains (32). The ability of aptamer B40 to bind to the coreceptor-binding site without prior ligation of CD4 by gp120, in contrast to the CD4 dependence of the binding of CD4i antibodies and of CCR5 itself, may result from a combination of its small size and high charge density and confer on it remarkable antiviral potential.
ACKNOWLEDGMENTS
This work was supported by the Wellcome Trust. A.K.D. was funded by a Felix Scholarship.
We thank Peter D. Kwong and Chih-chin Huang for helpful discussions and for supplying the Yu2 core constructs and George Gao for supplying the Ba-L gp120 expression vector.
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Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892
ABSTRACT
We recently described the isolation and structural characterization of 2'-fluoropyrimidine-substituted RNA aptamers that bind to gp120 of R5 strains of human immunodeficiency virus type 1 and thereby potently neutralize the infectivity of phylogenetically diverse R5 strains. Here we investigate the physical basis of their antiviral action. We show that both N-linked oligosaccharides and the variable loops V1/V2 and V3 are not required for binding of one aptamer, B40, to gp120. Using surface plasmon resonance binding analyses, we show that the aptamer binds to the CCR5-binding site on gp120 in a relatively CD4-independent manner, providing a mechanistic explanation for its neutralizing potency.
TEXT
The entry of human immunodeficiency virus type 1 (HIV-1) into target cells depends on the sequential interaction of the surface envelope glycoprotein, gp120, with the primary receptor, CD4, and the coreceptor, CCR5 (1, 5, 7, 9, 15, 29), and is an attractive target for antiviral intervention. However, gp120 is a challenging target because of the conformational flexibility and sequence variability it has evolved to counter host antibody responses (21, 37). The CD4-binding site is recessed and partially obscured from antibody access by the V1/V2 hypervariable loops and associated carbohydrate structures (37). Considerable conformational flexibility of the CD4-unbound state of gp120 further masks the conserved receptor binding site and hinders antibody access (19, 22, 27). The coreceptor binding site on gp120, which is thought to be composed of highly conserved elements of the ?19 strand and parts of the V3 hypervariable loop (28, 29, 39), is also protected by the V1/V2 loops from antibody access in the CD4-unbound state (4, 34, 36). Binding of CD4 induces conformational changes in gp120 that include the displacement of the V1/V2 stem-loop structure, thereby revealing a functional coreceptor binding site (26, 31, 38). Recent studies have also shown that this CD4-induced (CD4i) epitope on gp120 is protected from antibodies by steric hindrance and that the size of the neutralizing agent directed towards this region inversely correlates with its ability to neutralize (22). Mutational variation and extensive glycosylation of gp120 further add to the ineffectiveness of the humoral immune response.
In response to these challenges, one rational antiviral approach is to generate small ligands directed to the conserved core regions of gp120 that are critical for viral entry. We hypothesized that aptamers (11, 35), by virtue of their small size compared to antibodies, could access the conserved pockets in gp120, thereby blocking infection. Recently, we isolated and structurally characterized 2'F RNA aptamers that bind gp120 of HIV-1 R5 strain Ba-L and potently neutralize a broad range of HIV-1 primary isolates (8, 18). In order to test whether, as we had hypothesized, the mechanism of neutralization by aptamers was via ligation of critical receptor-binding regions, we mapped the binding site of one such aptamer (B40) using competition with gp120's natural ligands by deletion analysis and by deglycosylation.
The binding of aptamer to gp120 is independent of N-linked glycosylation. Recombinant monomeric Ba-L gp120 was expressed in insect cells using a baculovirus expression system, as previously described (18, 24), and deglycosylated using endo-?-N-acetylglucosaminidase H (endo H) under native conditions as previously described (20). When analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, endo H treatment of gp120 was found to have resulted in the complete disappearance of the fully glycosylated material (apparent mass 92 kDa) with the majority now migrating with an apparent mass of 63 kDa (Fig. 1A). Equimolar amounts of both the glycosylated and deglycosylated gp120 were then analyzed for ability to bind aptamer by BIAcore surface plasmon resonance analysis as previously described (8). The aptamer bound to both glycosylated and deglycosylated gp120 in a similar manner (Fig. 1B), indicating that the aptamer binding site on gp120 is not dependent on the N-linked glycans on the glycoprotein.
In contrast, monoclonal antibody (MAb) 2G12, which binds to a glycan-dependent epitope (30, 33), bound to the glycosylated but not the deglycosylated gp120, as expected (Fig. 1C). Soluble CD4 (sCD4) bound better to the glycosylated gp120 than to the deglycosylated gp120 (Fig. 1D), consistent with previous reports (16, 23, 25).
Role of V1/V2 and V3 loops in aptamer binding. Our results, above, show that one can remove the majority of N-linked sugars from gp120 without affecting the binding site for aptamer B40 but do not exclude the possibility that a specific oligosaccharide, refractory to deglycosylation, plays an important role. The most likely candidates for such an oligosaccharide lie within the hypervariable loops V1/V2 and V3, as they would be close to sites already known to be important for interaction of gp120 with receptor and coreceptor molecules (21, 28, 29, 37). In order to simultaneously investigate this possibility of a residual oligosaccharide and the potential that aptamers bound directly to these loop sequences, we used protein constructs, derived from the R5 strain Yu2, in which one or both loops are deleted and which lack complex sugars (17, 38). The results of a BIAcore analysis (Fig. 2C) show that aptamer B40 binds to the loop-deleted core of gp120, and therefore its binding site does not lie within the V1/V2 or V3 loops nor the oligosaccharides associated with them. It appears that the aptamer binding site is somewhat obscured in the core as well as the core plus V3 constructs of gp120 and becomes fully exposed only following the binding of CD4 (Fig. 2B and 2C). This is in marked contrast to the ability of aptamer B40 to bind full-length gp120 in a largely CD4-independent manner (Fig. 2A and Table 1). We tentatively conclude from this that the aptamer binding site, though located in core regions of gp120, is maintained in its aptamer-competent state by multiple interloop interactions and further that, when deletion of the loop causes the partial collapse of the aptamer binding pocket, the binding of CD4 allosterically reopens the pocket.
The aptamer binding site overlaps with the CCR5-binding site on gp120. We have previously shown that aptamer B4, which shares critical structural motifs with aptamer B40, competes for binding to gp120 with the CD4i MAb 17b (8, 18). Taken together with the CD4 dependence of efficient binding of aptamer B40 to the core gp120 construct described above, this pointed strongly to the possibility that the aptamer binding site was close to that of CCR5. To test this, we carried out a BIAcore surface plasmon resonance competition binding analysis using the 22-mer tyrosine-sulfated peptide (S-peptide) corresponding to the N terminus of CCR5 that acts as an essential component of the coreceptor for HIV-1 on the host cell (2, 6, 10, 12-14), as well as an unsulfated version of the same peptide (C-peptide) and sCD4, as alternate immobilized binding partners for gp120 (Fig. 3). As controls, RANTES, a physiological ligand for both CCR3 and CCR5 (3), and the anti-CCR5 MAb 3A9, but not monocyte chemoattractant protein 1, a ligand for CCR2 and CCR4, bound to the CCR5 peptides, as expected (data not shown).
Monomeric Ba-L gp120 bound well to immobilized CD4 but weakly to the S-peptide and negligibly to the C-peptide (Fig. 3A). However, preincubating gp120 with CD4 increased binding to both CCR5 peptides by more than 2.5-fold (Fig. 3B), confirming the CD4-induced binding property of the peptides to gp120 (14). Moreover, the approximately threefold difference in binding of both gp120 and gp120-sCD4 complex to the S-peptide versus C-peptide confirm that the tyrosine sulfate moieties contribute substantially to the association of the gp120-sCD4 complexes, as previously reported (13). As expected, the gp120-sCD4 complex did not bind to immobilized CD4 (Fig. 3B). When the gp120-17b MAb complex was injected, it bound only to the immobilized CD4 and not to the CCR5 peptides (data not shown), also as expected. The gp120-aptamer B40 complex also bound to the immobilized CD4 but not to the CCR5 peptides (Fig. 3C). The ternary complexes, gp120-sCD4-17 MAb (data not shown) and gp120-sCD4-aptamer B40 (Fig. 3D), did not bind to either the immobilized CCR5 peptides or human CD4.
Taken together, these results show that the aptamer competes with the N-terminal ectodomain of CCR5 for binding to gp120 and hence the aptamer binding site (aptatope) must overlap with the basic, conserved, coreceptor-binding region on gp120. This is consistent with the inability of aptamer B40 to bind the gp120 of the X4 strain, HXB2 (data not shown) in contrast to aptamers raised against X4 strains (32). The ability of aptamer B40 to bind to the coreceptor-binding site without prior ligation of CD4 by gp120, in contrast to the CD4 dependence of the binding of CD4i antibodies and of CCR5 itself, may result from a combination of its small size and high charge density and confer on it remarkable antiviral potential.
ACKNOWLEDGMENTS
This work was supported by the Wellcome Trust. A.K.D. was funded by a Felix Scholarship.
We thank Peter D. Kwong and Chih-chin Huang for helpful discussions and for supplying the Yu2 core constructs and George Gao for supplying the Ba-L gp120 expression vector.
REFERENCES
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